Desiccation Sensitivity and Germination of Recalcitrant Garcinia kola Heckel Seeds

Asomaning, Joseph M.; Olympio, Nana S.; Sacandé, M.

doi:10.3923/rjss.2011.15.27

Cited by 20 publications

(20 citation statements)

References 7 publications

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“…This indicates that measurement of leachate conductivity can be considered as a valuable tool for viability test in C. anomala seeds, as previously reported for many others species as Garcinia kola (Asomaning et al, 2011) and Vicia sativa (Samarah, 2006). Electrolyte leakage increased abruptly as seeds were dried below the critical moisture level and reached its maximum value at the lethal moisture level, corroborating results obtained from seeds of Euterpe edulis (Panza et al, 2007) and Syzygium cuminii .…”

Section: Discussionsupporting

confidence: 86%

“…When fresh recalcitrant seeds begin to dry, their viability is first slightly reduced as moisture is lost, but begins to decline considerably at a certain moisture level termed the critical moisture content or lowest safe moisture content Wesley-Smith et al, 2001). The value of 50.28% recorded in the present study as the critical moisture content is higher than those reported for many other recalcitrant tree seeds such as Garcinia kola seeds (Agyili et al, 2007;Asomaning et al, 2011) and Corypha umbraculifera seeds (Viji et al, 2013), but similar to values which have been reported for Archontophoenix alexandrae (Martins et al, 2003) and Euterpe edulis (Panza et al, 2007) seeds. This is an indication that the critical moisture content of recalcitrant seeds varies greatly among species.…”

Section: Discussionsupporting

confidence: 78%

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Germination of <i>Cola anomala</i> (K. Shum.) Shott and Endl seeds: effects of provenance, substrate and dehydration

Kanmegne¹,

Anouma²,

Fotso³

et al. 2015

Int. J. Bio. Chem. Sci

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As a step in the process of Cola anomala domestication, investigations were undertaken on germination requirements and desiccation tolerance of its seeds. Three seed provenances (Bamenda, Bayangam and Dschang), three substrates (forest top soil, river sand and a mixture of forest top soil and river sand) and two photoperiods (12 hours/day and, continuous darkness) were investigated for their effects on seed germination. To evaluate their desiccation tolerance, fresh seeds were dried at room temperature for 16 days during which seed moisture content, seed germination percentage and electrical conductivity of seed leachate were monitored at two-day intervals. Results showed that germination percentages were significantly (P < 0.05) higher both on forest top soil alone (86.04 ± 4.8%), and on a mixture of forest top soil and river sand (83.56 ± 4.5%), than on river sand alone (69.96 ± 4.7%). Seeds from Bamenda showed a higher germination percentage (91.4 ± 4.7%) than those from either Bayangam (77.36 ± 4.7%) or Dschang (70.8 ± 4.8%). The desiccation tolerance test revealed that as response to drying, the mean germination percentage was first slightly reduced as moisture was lost, then declined considerably at moisture content below 50.28%. Total germination failure was observed when seed moisture reached 32.24%. Electrical conductivity of seed leachate exhibited a strong correlation with loss of viability as well as with desiccation. It is concluded that there is a provenance-related variation in C. anomala seed germination. The best substrate for germination is forest top soil supplemented or not with river sand in a 1/1 (v/v) ratio. C. anomala seeds are desiccation-sensitive and their storage behaviour is recalcitrant.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionsupporting

confidence: 78%

Germination of <i>Cola anomala</i> (K. Shum.) Shott and Endl seeds: effects of provenance, substrate and dehydration

Kanmegne¹,

Anouma²,

Fotso³

et al. 2015

Int. J. Bio. Chem. Sci

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“…'garcinia-type' seeds the primary root emerges from one pole of the seed, and the shoot from the opposite pole (Ha et al 1988;Malik et al 2005;Joshi et al 2006;Asomaning et al 2011;Ofori et al 2015). Stress factors have been found to induce genome reprogramming of somatic cells to embryogenic cells (Verdeil et al 2007), and mechanical injury or shoot death could be the responsible stress factors for regeneration of roots and shoots in E. stipitata seeds.…”

Section: Fig 2 Root and Shoot Development Of Eugenia Stipitata Seedmentioning

confidence: 95%

Analyses of several seed viability markers in individual recalcitrant seeds of Eugenia stipitata McVaugh with totipotent germination

et al. 2016

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The use of biochemical seed viability markers is often compromised by the unknown partitioning of analytes in bulk seed lots consisting of inseparable populations of viable and nonviable seeds. We took advantage of an unusual morphological syndrome found in the recalcitrant, undifferentiated seeds of Eugenia stipitata: one seed can be cut into several parts, each of which can germinate and develop into seedlings. We used four seed parts from one individual seed to analyse seed moisture content (MC), seed viability and the antioxidant glutathione (γ-glutamyl-cysteinyl-glycine; GSH), glutathione disulphide (GSSG) and intermediates of glutathione synthesis and breakdown. Seeds were exposed to different environmental MC to induce various levels of desiccation stress. Upon storage at high seed MC, seed viability was maintained, while GSH concentration increased and the glutathione half-cell reduction potential (E ) was less negative than -215 mV, indicating GSH production and highly reducing conditions. Storage at low seed MC led to loss of GSH, resulting in a shift in E , and seed death. In contrast, the cyst(e)ine half-cell reduction potential (E ) could not distinguish between the viability categories. Previous studies on seed populations revealed that the probability for a seed being alive is 50% at E values between -180 and -160 mV. The single seed approach revealed that the window in which seed viability was lost could be slightly shifted towards more negative values. We discuss the contribution of cellular pH to E and recommend E. stipitata as a recalcitrant seed model to study stress response on a single seed basis.

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“…Beberapa penelitian daya hantar listrik untuk mendeteksi kualitas fisiologis benih tanaman kehutanan antara lain Pinus taeda L., P. Eliotti Engelm, P. pasutris Mill, P. Echinata Mill, P. strobus L (Bonner, 1991), E. cyclocarpum dan Paraserianthes falcataria (Nugroho, 1998), Gmelina arborea (Siskasari, 2001), R. apiculata (Rohandi dan Widyani, 2010), Garcinia kola Heckel (Asomaning et al, 2011), Vatica chinensis L. (Sukesh and Chandrashekar, 2013) serta Caesalpinia echinata Lam. (Neto et al, 2014).…”

Section: Moisture Content (Mc) and Germination Value (Gv) Of S Stementioning

confidence: 99%

Perubahan Fisiologis Dan Biokimia Benih Tengkawang Selama Penyimpanan

Rohandi¹,

Widyani²

2016

Jur. Pen. Eko. Dip.

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ABSTRAKPenelitian ini bertujuan untuk menganalisis aspek fisiologi dan biokimia perubahan fisik dan fisiologis benih tengkawang (Shorea stenoptera) selama proses penyimpanan. Percobaan penyimpanan dilakukan dengan menggunakan rancangan acak lengkap (RAL) faktorial 2 x 5 dengan 3 kali ulangan. Faktor-faktor tersebut meliputi suhu ruang simpan dan lama penyimpanan. Suhu ruang simpan terdiri dari ruang kamar (28-29 0 C) dan ruang AC (18-20 0 C), sedangkan lama penyimpanan terdiri dari 0, 1, 2, 3 dan 4 minggu. Parameter fisiologis yang diamati meliputi daya berkecambah, kecepatan berkecambah dan nilai perkecambahan, sedangkan parameter biokimia meliputi kandungan pati, lemak, protein dan daya hantar listrik. Hasil penelitian menunjukkan bahwa perlakuan lama penyimpanan memberikan pengaruh sangat nyata terhadap perubahan fisiologis (daya berkecambah, kecepatan berkecambah dan nilai perkecambahan) benih S. Stenoptera, yang dapat menyebabkan viabilitas benih terus menurun dengan semakin lamanya periode penyimpanan. Perbedaan suhu ruang simpan pada penyimpanan benih tengkawang (KA awal 46,06%) tidak menunjukkan perbedaan nyata. Sampai minggu ke 4 daya berkecambah benih di ruang kamar mampu dipertahankan sebesar 44% dan di ruang AC sebesar 49,33%. Kandungan lemak dan nilai daya hantar listrik cenderung mengalami peningkatan, sedangkan kandungan pati dan protein pada benih tengkawang cenderung mengalami penurunan selama penyimpanan. Kata kunci : biokimia, fisiologis, perubahan, penyimpanan, tengkawang ABSTRACTThis research aimed to analyze physiological and biochemical changes of tengkawang (S. stenoptera) seeds during storage process. The experimental design used 2 x 5 factorial in completely randomized design with 3 replications. These factors consisted of storage temperatures (ambient room (28-29 0 C) and ) and periods of storage (0, 1, 2, 3 and 4 weeks

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Desiccation Sensitivity and Germination of Recalcitrant Garcinia kola Heckel Seeds

Cited by 20 publications

References 7 publications

Germination of <i>Cola anomala</i> (K. Shum.) Shott and Endl seeds: effects of provenance, substrate and dehydration

Germination of <i>Cola anomala</i> (K. Shum.) Shott and Endl seeds: effects of provenance, substrate and dehydration

Analyses of several seed viability markers in individual recalcitrant seeds of Eugenia stipitata McVaugh with totipotent germination

Perubahan Fisiologis Dan Biokimia Benih Tengkawang Selama Penyimpanan

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