Desenvolvimento e validação de metodologia para quantificação de alcalóides totais como berberina em fitoterápico contendo Berberis vulgaris L.

Oliveira, Marcos André Cunha de; Albuquerque, Manoel Bandeira de; Xavier, Haroudo Sátiro; Strattmann, Ruth R.; Júnior, Severino Grangeiro; Queiroz, Adelaide T.

doi:10.1590/s0102-695x2006000300013

Cited by 11 publications

(5 citation statements)

References 7 publications

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“…A "tintura do algodoeiro" is used as a component in a phytotherapeutic agent (Robuterina ® ), which is used to treat menstrual cycle disorders. This medicine further includes Berberis vulgaris L., and Gossypium herbaceum L. It also acts as an emmenagogue, hemostatic and ocitocic and has anti-inflammatory activity (Oliveira et al, 2006). Preclinical toxicity of a phytotherapeutic preparation containing Gossypium herbaceum (Cotton Plant) was tested by Mello et al (2008), whose results revealed the absence of systemic toxicity at a therapeutic dose.…”

Section: Plants Used In Screening and Their Pharmacological Activitiesmentioning

confidence: 99%

Acetylcholinesterase inhibition by somes promising Brazilian medicinal plants

Feitosa¹,

Freitas²,

Luz³

et al. 2011

Braz. J. Biol.

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A microplate assay and a thin-layer chromatography (TLC) "in situ" assay based on the Ellman assay was used to screen for acetylcholinesterase inhibitors from ethyl acetate and methanol extracts of Brazilian medicinal plants of families that, according to the literature, have traditional uses that might be connected with acetylcholinesterase inhibition. Eighteen species belonging to Convolvulaceae, Crassulaceae, Euphorbiaceae, Leguminosae, Malvaceae, Moraceae, Nyctaginaceae and Rutaceae families were tested. The most active plants were Ipomoea asarifolia (IC 50 = 0.12 mg/mL), Jatropha curcas (IC 50 = 0.25 mg/mL), Jatropha gossypiifolia (IC 50 = 0.05 mg/mL), Kalanchoe brasiliensis (IC 50 = 0.16 mg/mL) and Senna alata (IC 50 = 0.08 mg/mL). The most promising extracts were the Jatropha gossypiifolia and Senna alata species assuming there were compounds with a similar activity to galanthamine, which should contain about 1% of an active compound, or if present at lower levels even more active compounds than galanthamine (IC 50 = 0.37 x 10 -3 mg/ mL) should be present.Keywords: Brazilian medicinal plants, Alzheimer's disease, acetylcholinesterase inhibitors, microplate assay. Plantas medicinais brasileiras promissoras para inibição da acetilcolinesterase ResumoOs ensaios de microplaca e cromatografia em camada delgada com base no ensaio de Ellman foram usados para triagem de inibidores da acetilcolinesterase dos extratos acetato de etila e metanol de plantas medicinais brasileiras de famílias que, segundo a literatura, tem usos tradicionais que podem estar relacionadas com a inibição da acetilcolinesterase, enzima associada ao mal de Alzheimer. Dezoito plantas das famílias: Convolvulaceae, Crassulaceae, Euphorbiaceae, Leguminosae, Malvaceae, Moraceae, Nyctaginaceae e Rutaceae foram testadas. As espécies mais ativas foram Ipomoea asarifolia (CI 50 = 0,12 mg/mL), Jatropha curcas (CI 50 = 0,25 mg/mL), Jatropha gossypiifolia (CI 50 = 0,05 mg/mL), Kalanchoe brasiliensis (CI 50 = 0,16 mg/mL) e Senna alata (CI 50 = 0,08 mg/mL). Os extratos mais promissores foram os das espécies Jatropha gossypiifolia e Senna alata, assumindo a presença de compostos com atividade semelhante à galantamina que deve conter cerca de 1% de um composto ativo, ou se presentes em menores níveis ainda mais compostos ativos que a galantamina (CI 50 = 0,37 x10 -3 mg/mL) devem estar presentes.Palavras-chave: plantas medicinais brasileiras, doença de Alzheimer, inibidores da acetilcolinesterase, microplaca.

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Section: Plants Used In Screening and Their Pharmacological Activitiesmentioning

confidence: 99%

Acetylcholinesterase inhibition by somes promising Brazilian medicinal plants

Feitosa¹,

Freitas²,

Luz³

et al. 2011

Braz. J. Biol.

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“…All aqueous extracts obtained were subjected to analysis to determine the contents of phenolic compounds (Djeridane et al, 2006), total alkaloids (Oliveira et al, 2006) and antioxidant activity (Kumaran & Karunakaran, 2006).…”

Section: Methodsmentioning

confidence: 99%

“…For each sample, the spectrophotometer reading was performed at 435 nm wavelength. Blank was obtained by mixing nitric acid and thiourea (Oliveira et al, 2006).…”

Section: Methodsmentioning

confidence: 99%

Post-harvesting of Solanum paniculatum L. leaves. Part II: Antioxidant activity and chemical composition

Martins

Goneli

Cardoso

et al. 2021

Rev. bras. eng. agríc. ambient.

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‘Jurubeba’ (Solanum paniculatum L.) is a medicinal plant used in traditional medicine for liver problems, in addition to being used as a cholagogue, emmenagogue, healing agent, febrifuge, anti-inflammatory, antipyretic, tonic, decongestant, diuretic and against inappetence. Thus, the objective of this study was to evaluate the antioxidant activity, concentration of phenolic compounds and total alkaloids of aqueous extracts prepared from ‘Jurubeba’ leaves subjected to drying. ‘Jurubeba’ leaves were dried at different temperatures (40, 50, 60 and 70 °C) and velocities (0.4 and 0.8 m s-1) of the drying air. Aqueous extracts of the leaves, subjected to drying, were evaluated by absorbance reading in a spectrophotometer. Increase in drying air temperature reduced the contents of total phenolics and total alkaloids of the leaf aqueous extracts, whereas increment in air velocity increased the concentration of these substances. Antioxidant activity was not influenced by the increase in air temperature at air velocity of 0.4 m s-1, but under air velocity of 0.8 m s-1 there was reduction with increasing temperature. Drying of ‘Jurubeba’ leaves can be carried out with temperature of 40 or 50 °C and drying air velocity of 0.4 m s-1.

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“…The analysis of the total alkaloid content was determined according to the methodology described by Oliveira et al [20]. For this, two g of plant material was reacted with eight mL of 10% acetic acid, prepared in ethanol, for four h, filtered and precipitated with concentrated ammonium hydroxide.…”

Section: Analysis Of the Alkaloid Contentsmentioning

confidence: 99%

Biological potentialities and chemical composition of Tarenaya aculeata roots and stems

Ferreira Duarte,

Aguayo De Castro,

Sobrinho Ventura

et al. 2023

Rev. Colomb. Quim.

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Plant extracts are rich in secondary metabolites responsible for numerous biological activities. This study aimed to evaluate the antioxidant, antibacterial and photoprotective potentials, toxicity and chemical composition of extracts and fractions of stems and roots of Tarenaya aculeata. Phytochemical analyses were performed at qualitative and quantitative levels to evaluate the classes of secondary metabolites. The sun protection factor (SPF) and antioxidant potentials were determined spectrophotometrically, the antibacterial activity was tested against seven bacteria and the toxicity was evaluated using Artemia salina assay. Phytochemical screening revealed the presence of alkaloids, phenolic compounds, flavonoids, glycosides, tannins and saponins. The levels of phenolic compounds, tannins and alkaloids, SPF and antioxidant potentials showed greater results in the stem (SF) and root (RF) fractions in relation to the stem (SE) and root (RE) extracts. All samples exhibited a broad spectrum of antibacterial activity, with MIC values ranging from 31.25 to 250 μg mL-1. SE, SF and RF caused mortality in A. salina larvae, with LC50 of 347.06, 34.71 and 85.39 μg mL-1, respectively, whereas RE was non-toxic. Thus, T. aculeata is rich in bioactive secondary metabolites, although further studies will be needed to characterize its chemical constituents and investigate their adverse effects.

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Desenvolvimento e validação de metodologia para quantificação de alcalóides totais como berberina em fitoterápico contendo Berberis vulgaris L.

Cited by 11 publications

References 7 publications

Acetylcholinesterase inhibition by somes promising Brazilian medicinal plants

Acetylcholinesterase inhibition by somes promising Brazilian medicinal plants

Post-harvesting of Solanum paniculatum L. leaves. Part II: Antioxidant activity and chemical composition

Biological potentialities and chemical composition of Tarenaya aculeata roots and stems

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