Desenvolvimento de um spot test para o monitoramento da atividade da peroxidase em um procedimento de purificação

Zeraik, Ana Eliza; Souza, Fernanda Sant'Ana de; Fatibello‐Filho, Orlando; Leite, Oldair Donizeti

doi:10.1590/s0100-40422008000400003

Cited by 83 publications

(31 citation statements)

References 13 publications

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“…(4 °C), and supernatant was used for determination of activity of the enzymes. The activity of the POD enzyme was determined by absorbance of the reaction solution, at 560 nm, following the methodology described by Zeraik et al (2008); whereas the SOD enzyme activity was determined at 480 nm (Mccord and Fridovich, 1969). Results were expressed as unit of enzyme per milligram protein (U.mg -1 protein).…”

Section: Determination Of the Seed Tolerance To Weathering Damage (Wd)mentioning

confidence: 99%

Susceptibility to weathering damage and oxidative stress on soybean seeds with different lignin contents in the seed coat

et al. 2016

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-This study aimed at evaluating the relationship among lignin content in the seed coat of soybean seeds with tolerance to weathering damage and oxidative stress. Four soybean cultivars (CD 208, CD 215, CD 202 and BRS 184), with lignin contents in the seed coat of 4.2, 2.6, 2.0 and 1.7%, respectively, were used. Seeds were subjected to deterioration by moisture, through artificially simulated rains. Pods of all cultivars were subjected to irrigation by constant sprinkling during 4 hours, and then dried in oven, set at 35 °C, for 37 hours; repeating twice such procedure. After these treatments seeds were subjected to tests and then compared to the control. Tests performed were: germination, accelerated aging and tetrazolium test; thereby quantifying the occurrence of damages by weathering damage. The activity of enzymes superoxide dismutase and guaiacol peroxidase was measured; and the lipid peroxidation was assessed. Soybean cultivars CD 208 and CD 215, with higher lignin content in the seed coat, were less susceptible to weathering damage. Seeds of cultivar CD 208 have also presented lower oxidative stress, when subjected to weathering damage stress.Index terms: Glycine max. L, field deterioration, seed quality.Suscetibilidade à deterioração por umidade e ao estresse oxidativo em sementes de soja com variação no conteúdo de lignina no tegumento RESUMO -O estudo teve como objetivo avaliar a relação entre o teor de lignina no tegumento de sementes de soja com tolerância à deterioração por umidade e ao estresse oxidativo. Foram utilizadas quatro cultivares de soja: CD 208, CD 215, CD 202 e BRS 184, com teores de lignina no tegumento de 4,2; 2,6; 2,0 e 1,7%, respectivamente. As sementes foram submetidas a uma deterioração por umidade através de chuvas simuladas (4 horas), e, posteriormente, secas em estufa (35 °C), por 37 horas, totalizando dois ciclos de umedecimento e secagem. Foram realizados os testes: germinação, envelhecimento acelerado e teste de tetrazólio. Foi medida a atividade das enzimas antioxidantes: Superóxido Dismutase e Guaiacol Peroxidase; e a peroxidação lipídica. As avaliações foram realizadas no delineamento inteiramente casualizado, em esquema fatorial 2x4, com quatro repetições. As cultivares de soja CD 208 e CD 215, com maior teor de lignina no tegumento, são menos suscetíveis ao dano por umidade. As sementes da cultivar CD 208 também apresentaram menor estresse oxidativo, quando submetidas a deterioração por umidade.Termos para indexação: Glycine max. L, deterioração em campo, qualidade de sementes.

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Section: Determination Of the Seed Tolerance To Weathering Damage (Wd)mentioning

confidence: 99%

Susceptibility to weathering damage and oxidative stress on soybean seeds with different lignin contents in the seed coat

et al. 2016

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“…The solution changed from no-color to dark yellow for chitosan/HRP film and light yellow for chitosan/AuNPs/HRP film. These results indicate that the HRP has enzymatic activity in the films [18]. Differences between color change of the chitosan/ HRP and chitosan/AuNPs/HRP films indicate that the AuNPs in films could alter the enzymatic activity of horseradish peroxidase.…”

Section: Enzyme Activity Of Hydrogen Peroxide Immobilized In Films: Gmentioning

confidence: 86%

“…The color change in the solutions was monitored visually for 5 min. All solutions were prepared using a buffer solution (0.1 m and pH = 6.8) as solvent [18].…”

Section: Enzyme Activity Of Hydrogen Peroxide Immobilized In Films: Gmentioning

confidence: 99%

A new conductometric biosensor based on horseradish peroxidase immobilized on chitosan and chitosan/gold nanoparticle films

Valencia¹,

Vercik

2014

Journal of Polymer Engineering

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A new conductometric biosensor based on horseradish peroxidase immobilized on chitosan and chitosan/gold nanoparticle films Abstract: A new conductometric biosensor was developed and characterized; the biosensor was based on horseradish peroxidase that was deposited in chitosan and chitosan/AuNPs films. The biosensors were characterized by scanning electron microscopy and current-voltage curves. Current-voltage curves in biosensors showed that the electrical conductivity and bistability in biosensors can be modulated by horseradish peroxidase.

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“…A esta se adicionou 0,5 mL de ácido perclórico 1,4%, deixando-se em repouso por 10 minutos e posterior leitura a 395 nm (Duangmal & Apenten, 1999). A atividade da enzima polifenoloxidase foi calculada usando a mesma equação para o calculo da atividade da peroxidase, citada anteriormente (Putter, 1974;Duangmal & Apenten, 1999;Zeraik et al, 2008):…”

Section: Methodsunclassified

Estudo do potencial fitotóxico de extratos de Bauhinia ungulata L. sobre a divisão celular e atividade enzimática em plântulas de alface

Paula

Canteli

Silva

et al. 2015

Rev. bras. plantas med.

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RESUMO:Este trabalho visou avaliar a ação fitotóxica de extrato e frações obtidas das folhas de Bauhinia ungulata L sobre a divisão celular e atividade das enzimas α-amilase, catalase, peroxidase e polifenoloxidase em plântulas de alface (Lactuca sativa, var Grand rapis). Sementes de alface foram expostas às concentrações de 250, 500 e 1000 µg.mL -1 do extrato de Bauhinia ungulata e frações do mesmo, em condições ambientais controladas. Após três dias de protusão radicular, as raízes das plântulas foram cortadas e submetidas a preparo específico para visualização e contagem do número de células em cada fase mitótica. No sétimo dia, as amostras foram submetidas à avaliação da atividade enzimática utilizando técnica específica para cada enzima e leitura espectrofotométrica. Na análise da divisão celular observou-se que houve redução no número de células em mitose nas raízes das plântulas testadas, e a fração acetato de etila apresentou maior efeito inibitório sobre a divisão celular. O efeito sobre as enzimas que demonstram alterações no sistema de defesa antioxidante de L. sativa mostram que o extrato bruto e frações alteraram a produção das enzimas α-amilase, peroxidase, catalase e polifenol oxidase em, ao menos, uma das concentrações testadas, sendo um indicativo de estresse que interfere na divisão celular. Os resultados obtidos apontam para a presença de compostos com atividade inibidora ou estimulatória nas folhas de B. ungulata revelando potencial fitotóxico sobre as plântulas de alface. Palavras-chave:Bauhinia ungulata, catalase, peroxidase.ABSTRACT: Study of phytotoxic potential of extracts of Bauhinia ungulata L. on cell division and enzyme activity in lettuce seedlings. Theaim of this studywas to evaluate thephytotoxic actionofextractand fractionsobtainedfrom the leaves ofBauhiniaungulata L. inbioassaylaboratory, assessing their interferenceon cell division andactivity of the enzymesalpha-amylase, catalase, peroxidase andpolyphenoloxidaseofLactucasativa(lettu ce). The lettuce seedswere exposedto concentrationsof 250,500and 1000μg.mL -1of the extracts and fractions of Bauhinia ungulataunder controlled environmental conditions. After three days ofrootradicleprotrusion, the plants` rootswere cutand subjected tospecific preparationfor visualization andcounting of the number ofcells in eachmitotic stage. On the seventh daythe sampleswere subjected toenzymatic activityusingspecific techniquefor eachenzyme anda spectrophotometer measure. In the analysisof the mitotic indexit was observedthatthere was reduction in the number of cells in mitosis in the roots of the plants tested and thattheethyl acetate fractionsignificantly affected thecell division. The effecton the enzymesthat showed changesinthe antioxidant defensesystemof Lactucasativaindicate thatthe crude extractand the fractionsaltered theproductionofα-amylase, peroxides, catalaseand polyphenol enzymesin at least oneof the testedconcentrations, representingan indicationstress,which interferesin the celldivision.Theobtained results point out the presence ...

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Desenvolvimento de um spot test para o monitoramento da atividade da peroxidase em um procedimento de purificação

Cited by 83 publications

References 13 publications

Susceptibility to weathering damage and oxidative stress on soybean seeds with different lignin contents in the seed coat

Susceptibility to weathering damage and oxidative stress on soybean seeds with different lignin contents in the seed coat

A new conductometric biosensor based on horseradish peroxidase immobilized on chitosan and chitosan/gold nanoparticle films

Estudo do potencial fitotóxico de extratos de Bauhinia ungulata L. sobre a divisão celular e atividade enzimática em plântulas de alface

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