Desenvolvimento de iniciadores para detecção e identificação de Diaporthe phaseolorum var. meridionalis em sementes de soja

Vechiato, M. H.; Maringoni, Antônio Carlos; Martins, Elza M. F.

doi:10.1590/s0100-54052006000200011

Cited by 3 publications

(1 citation statement)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[15]. The primers DphLe and DphRi were developed for detection of D. aspalathi [20]. There are also real-time (q)PCR assays based on ITS to detect and quantify Diaporthe spp.…”

Section: Genus Diaporthementioning

confidence: 99%

Diagnosis of Soybean Diseases Caused by Fungal and Oomycete Pathogens: Existing Methods and New Developments

Hosseini

Voegele

Link

2023

JoF

View full text Add to dashboard Cite

Soybean (Glycine max) acreage is increasing dramatically, together with the use of soybean as a source of vegetable protein and oil. However, soybean production is affected by several diseases, especially diseases caused by fungal seed-borne pathogens. As infected seeds often appear symptomless, diagnosis by applying accurate detection techniques is essential to prevent propagation of pathogens. Seed incubation on culture media is the traditional method to detect such pathogens. This method is simple, but fungi have to develop axenically and expert mycologists are required for species identification. Even experts may not be able to provide reliable type level identification because of close similarities between species. Other pathogens are soil-borne. Here, traditional methods for detection and identification pose even greater problems. Recently, molecular methods, based on analyzing DNA, have been developed for sensitive and specific identification. Here, we provide an overview of available molecular assays to identify species of the genera Diaporthe, Sclerotinia, Colletotrichum, Fusarium, Cercospora, Septoria, Macrophomina, Phialophora, Rhizoctonia, Phakopsora, Phytophthora, and Pythium, causing soybean diseases. We also describe the basic steps in establishing PCR-based detection methods, and we discuss potentials and challenges in using such assays.

show abstract

“…[15]. The primers DphLe and DphRi were developed for detection of D. aspalathi [20]. There are also real-time (q)PCR assays based on ITS to detect and quantify Diaporthe spp.…”

Section: Genus Diaporthementioning

confidence: 99%

Diagnosis of Soybean Diseases Caused by Fungal and Oomycete Pathogens: Existing Methods and New Developments

Hosseini

Voegele

Link

2023

JoF

View full text Add to dashboard Cite

show abstract

Diaporthe phaseolorum var. meridionalis (soyabean stem canker)

2022

CABI Compendium

View full text Add to dashboard Cite

show abstract

Sensibility of the PCR technique in the detection of Stenocarpella sp. associated with maize seeds

Barrocas

Machado

Almeida

et al. 2012

Rev. bras. sementes

View full text Add to dashboard Cite

-Maize seeds, infected by Stenocarpella species, are important sources of inoculum for the introduction and dissemination of stalk and ear rot and macrospore leaf spot diseases. the use of healthy seeds is an important strategy for the preventive control of these diseases. However, one of the difficulties in the health quality control programs for maize seeds is the availability of a reliable and quick method for detecting these fungi during routine seed analyses. Therefore, the objective of the present study was to investigate the possibility of using the PCR technique as an alternative method for accurately detecting these pathogens in maize seed samples. Maize seeds were kept in contact with S. maydis colonie developed in PDA media containing mannitol at -1.4 MPa for 72 h. the seed samples used in this study were prepared with infected seeds at incidences of 100, 20, 10, 2, 1 and zero %.the primers used were able to detect S. maydis fungi in association with seeds with a maximum of 2% , however those primers were not able to differentiate between the two species.Index terns: fungus, molecular detection, stalk and ear rot, macrospore leaf spot.Sensibilidade da técnica PCR na detecção de Stenocarpella sp. associados às sementes de milho RESuMo -Sementes de milho infectadas por Stenocarpella são importantes fontes de inóculo para a introdução e disseminação da podridão do colmo e da espiga e mancha foliar de macrospora em campos de produção. o uso de sementes sadias constitui uma importante estratégia para o controle preventivo dessas doenças. A disponibilidade de métodos rápidos para a detecção desses fungos em análises de rotina é uma das dificuldades em programas de controle de qualidade de sementes de milho, já que o método utilizado demanda longo tempo e sua identificação é bastante subjetiva. o objetivo desse trabalho foi investigar o uso da técnica PCR para a detecção desses patógenos em sementes de milho. Para isto sementes de milho inoculadas com S. maydis foram misturadas a sementes sadias de modo que formassem lotes com incidências de 100, 20, 10, 2, 1 e zero%. o par de primers utilizado para detectar S. maydis foi capaz de detectar o máximo de 2% de incidência, todavia os primers não foram suficientes para diferenciar as duas espécies.termos para indexação: fungo, detecção molecular, podridão do colmo e da espiga, mancha foliar de macrospora.

show abstract

Desenvolvimento de iniciadores para detecção e identificação de Diaporthe phaseolorum var. meridionalis em sementes de soja

Cited by 3 publications

References 17 publications

Diagnosis of Soybean Diseases Caused by Fungal and Oomycete Pathogens: Existing Methods and New Developments

Diagnosis of Soybean Diseases Caused by Fungal and Oomycete Pathogens: Existing Methods and New Developments

Diaporthe phaseolorum var. meridionalis (soyabean stem canker)

Sensibility of the PCR technique in the detection of Stenocarpella sp. associated with maize seeds

Contact Info

Product

Resources

About