Descriptions of Rhodopseudomonas parapalustris sp. nov., Rhodopseudomonas harwoodiae sp. nov. and Rhodopseudomonas pseudopalustris sp. nov., and emended description of Rhodopseudomonas palustris

Abstract

Descriptions ofT , JA447 and JA490) of red to reddish brown pigmented, rod-shaped, motile and budding phototrophic bacteria were isolated from soil and freshwater sediment samples from different geographical regions of India. All strains contained bacteriochlorophyll a and carotenoids of the spirilloxanthin series. The major cellular fatty acid of strains JA310 T and JA531 T was C 18 : 1 v7c, the quinone was Q-10 and polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, an aminohopanoid and an unidentified aminolipid. Phylogenetic analysis based on 16S rRNA gene sequences showed that all strains clustered with species of the genus Rhodopseudomonas in the class Alphaproteobacteria. Strains JA531 T , JA447 and JA490 were genotypically (.80 % related based on DNA-DNA hybridization) and phenotypically closely related to each other and the three strains were distinct from strain JA310 T (33 % related). Furthermore, all four strains had less than 48 % relatedness (DNA-DNA hybridization) with type strains of members of the genus Rhodopseudomonas, i.e. Rhodopseudomonas palustris ATCC 17001 T

Genomic and genetic sequence information of strains assigned to the genus Rhodopseudomonas reveal the great heterogeneity of the group and identify strain Rhodopseudomonas palustris DSM 123T as the authentic type strain of this species

“…More recently, two new species of the genus Rhodopseudomonas, Rhodopseudomonas harwoodiae and Rhodopseudomonas parapalustris, were described and their differentiation from other species of the genus by DNA-DNA hybridization analysis demonstrated similarity values below 50% [9]. This study also supported the recognition of R. rhenobacensis and R. faecalis as separate species and pointed out differences at the species level between the two references of the type of R. palustris, strains DSM 123 and ATCC 17001.…”

Photo-fermentation hydrogen production by Rhodopseudomonas sp. nov. strain A7 isolated from the sludge in a bioreactor

“…1 e Observations of the strain A7 using AFM (a, b) and TEM (c, d, e). i n t e r n a t i o n a l j o u r n a l o f h y d r o g e n e n e r g y 4 0 ( 2 0 1 5 ) 8 6 6 1 e8 6 6 8 strain A7 and Rhodopseudomonas palustris strain ATCC 17001 [24] is shown in Tables 1 and 2. The distinct difference present in the strain ATCC 17001 and strain A7 includes the main fatty acid components and substrate utilization.…”

Urban stream microbial communities show resistance to pharmaceutical exposure

“…Rhodopseudomonas pseudopalustris was only recently proposed as a species, having previously been considered a strain of Rhodopseudomonas palustris (Ramana et al. ) . Rhodopseudomonas palustris is a member of the alpha proteobacteria that is widely distributed in nature and is considered to be one of the most metabolically versatile bacterial species (Harwood and Gibson ).…”

“…Comparison of sequences from this unclassified genus with the NCBI 16S rRNA database indicated that the majority matched most closely with Rhodopseudomonas pseudopalustris. Rhodopseudomonas pseudopalustris was only recently proposed as a species, having previously been considered a strain of Rhodopseudomonas palustris (Ramana et al 2012). Rhodopseudomonas palustris is a member of the alpha proteobacteria that is widely distributed in nature and is considered to be one of the most metabolically versatile bacterial species (Harwood and Gibson 1988).…”