Description of Verrucosispora qiuiae sp. nov., isolated from mangrove swamp sediment, and emended description of the genus Verrucosispora

Abstract

A Micromonospora -like strain, RtIII47T, was isolated from a mangrove swamp in Sanya, Hainan Province, China. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that the strain had a close association with the genus Verrucosispora and shared the highest sequence similarity with Verrucosispora lutea YIM 013T (98.0 %). The strain also showed high 16S rRNA gene sequence similarities to Micromonospora olivasterospora DSM 43868T (97.9 %), Plantactinospora mayteni YIM 61359T (97.9 %), Salinispora tropica CNB-440T (97.8 %), Micromonospora peucetia DSM 43363T (97.7 %), Micromonospora auratinigra TT1-11T (97.7 %), Verrucosispora sediminis CGMCC 4.3550T (97.6 %) and Salinispora arenicola CNH-643T (97.5 %). Phylogenetic analysis based on the gyrB gene sequence supported the conclusion that strain RtIII47T should be assigned to the genus Verrucosispora . DNA–DNA relatedness between strain RtIII47T and the most closely related type strain, V. lutea YIM 013T, was less than 40 %. Chemotaxonomic results confirmed the taxonomic position of the isolate in the genus Verrucosispora , and revealed differences at the species level in polar lipids, whole-cell sugars and DNA G+C content. A combination of physiological and biochemical tests also distinguished this strain from other Verrucosispora species. Based on genotypic and phenotypic observations, strain RtIII47T ( = CGMCC 4.5826T = NBRC 106684T) is proposed as the type strain of a novel species, Verrucosispora qiuiae sp. nov. An emended description of the genus Verrucosispora is also provided.

Verrucosispora fiedleri sp. nov., an actinomycete isolated from a fjord sediment which synthesizes proximicins

“…The results of the present study confirm and extend those reported by Fiedler et al (2008) in showing that the proximicin-producing strain MG-37 T has chemotaxonomic, molecular and morphological properties in line with its classification in the genus Verrucosispora (Rheims et al 1998;Goodfellow et al 2012;Xi et al 2012 The description is based on data taken from this and an earlier study ).…”

“…The isolate was examined for chemotaxonomic and morphological properties characteristic for the genus Verrucosispora (Goodfellow et al 2012;Xi et al 2012). The arrangement of hyphae and spores were examined on an oatmeal agar (ISP medium 3; Shirling and Gottlieb 1966) plate which had been incubated at 28 o C for 2 weeks.…”

“…Ampicillin ( Determined by spectrophotometric method (Dai et al 2010); e. Determined by a modified fluorimetric microwell plate method (Xi et al 2012); f. Determined by using melting profiles in microplates (Xie et al 2012) …”

“…The genus Verrucosispora forms a distinct branch in the 16S rRNA Micromonosporaceae gene tree, and can be distinguished from other genera classified in this family by using chemotaxonomic and morphological features (Goodfellow et al 2012;Xi et al 2012) and genus-specific primers (Xie et al 2011). The taxon currently encompass six species, Verrucosispora gifhornensis (Rheims et al 1998), the type species, Verrucosispora lutea (Liao et al 2009), Verrucosispora maris (Goodfellow et al 2012), Verrucosispora qiuiae (Xi et al 2012), Verrucosispora sediminis (Dai et al 2010) and Verrucosispora wenchangensis (Xie et al 2012), the single members of which can be separated using a combination of genotypic and phenotypic procedures (Goodfellow et al 2012;Xi et al 2012;Xie et al 2012).…”

“…The taxon currently encompass six species, Verrucosispora gifhornensis (Rheims et al 1998), the type species, Verrucosispora lutea (Liao et al 2009), Verrucosispora maris (Goodfellow et al 2012), Verrucosispora qiuiae (Xi et al 2012), Verrucosispora sediminis (Dai et al 2010) and Verrucosispora wenchangensis (Xie et al 2012), the single members of which can be separated using a combination of genotypic and phenotypic procedures (Goodfellow et al 2012;Xi et al 2012;Xie et al 2012). The type strains of these species were isolated from a peat bog (V. gifhornensis), mangrove soils (V. lutea, V. qiuiae and V. wenchangensis) and deep-sea sediments (V. maris and V. sediminis).…”