Description of the larval morphology of captive reared Panulirus ornatus spiny lobsters, benchmarked against wild-caught specimens

Smith, Gregory G.; Salmon, Matt; Kenway, Matthew; Hall, Michael R.

doi:10.1016/j.aquaculture.2009.06.030

Cited by 43 publications

(25 citation statements)

References 22 publications

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“…Positive pathogen control nauplii and negative control nauplii were treated similarly, except 1×10 6 CFU mL −1 V. owensii DY05 or no bacteria were added, respectively. Apparently healthy P. ornatus phyllosomas, as assessed by photopositive response and active motility, were sourced from the AIMS larviculture facility [3], distributed to 12-well cell culture plates (1 larva well −1 ) and fed live enriched or non-enriched nauplii (t = 0 h). All treatments were performed in quintuplicate ( n = 60) and survival was assessed every 24 h for 5 days.…”

Section: Methodsmentioning

confidence: 99%

Identification of an Antagonistic Probiotic Combination Protecting Ornate Spiny Lobster (Panulirus ornatus) Larvae against Vibrio owensii Infection

et al. 2012

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Vibrio owensii DY05 is a serious pathogen causing epizootics in the larviculture of ornate spiny lobster Panulirus ornatus . In the present study a multi-tiered probiotic screening strategy was used to identify a probiotic combination capable of protecting P. ornatus larvae (phyllosomas) from experimental V. owensii DY05 infection. From a pool of more than 500 marine bacterial isolates, 91 showed definitive in vitro antagonistic activity towards the pathogen. Antagonistic candidates were shortlisted based on phylogeny, strength of antagonistic activity, and isolate origin. Miniaturized assays used a green fluorescent protein labelled transconjugant of V. owensii DY05 to assess pathogen growth and biofilm formation in the presence of shortlisted candidates. This approach enabled rapid processing and selection of candidates to be tested in a phyllosoma infection model. When used in combination, strains Vibrio sp. PP05 and Pseudoalteromonas sp. PP107 significantly and reproducibly protected P. ornatus phyllosomas during vectored challenge with V. owensii DY05, with survival not differing significantly from unchallenged controls. The present study has shown the value of multispecies probiotic treatment and demonstrated that natural microbial communities associated with wild phyllosomas and zooplankton prey support antagonistic bacteria capable of in vivo suppression of a pathogen causing epizootics in phyllosoma culture systems.

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Section: Methodsmentioning

confidence: 99%

Identification of an Antagonistic Probiotic Combination Protecting Ornate Spiny Lobster (Panulirus ornatus) Larvae against Vibrio owensii Infection

et al. 2012

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“…Phyllosoma were 32 days post-hatch at the commencement of the trial. Development stages were determined using the morphological features described by Smith et al (2009b). Based on a subsample of 150 individuals, the relative proportion of development stage for the phyllosoma in each tank at the beginning of the trial was 64% 3 (3), 35% 4 (1) and 1% 4 (2) [development stage (instar number)], with an average initial weight of 2.5 ± 0.1 mg.…”

Section: Methodsmentioning

confidence: 99%

“…Additionally, the slight increase in lipid content may also be ascribable to the longer retention times observed in both the hepatopancreas and hindgut of the phyllosoma fed the HFM diet as opposed to the other treatments, allowing longer time for total nutrient assimilation. The hepatopancreas has been identified as the primary organ involved in phyllosoma nutrient absorption and storage (Wang et al 2014c), while larger food particles that are rejected as pseudo-faeces pass directly through the hepatopancreas and into the hindgut (Smith et al, 2009b). Fast gut evacuation times have been suggested to correlate with poor nutrient absorption (Govoni et al, 1984), particularly protein utilisation (Tonheim et al, 2005), due to the short time available for efficient digestion and assimilation (Serrano, 2012).…”

Section: Hfmmentioning

confidence: 98%

The effects of pre-digested protein sources on the performance of early–mid stage Panulirus ornatus phyllosoma

et al. 2015

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“…P. ornatus phyllosomas were sourced from broodstock held at the Tropical Aquaculture Facility of the Australian Institute of Marine Science (AIMS), Townsville, Australia. Maintenance of broodstock, production of phyllosomas, and larviculture were performed according to the methods of Smith et al (41). Only apparently healthy individuals as assessed by positive phototaxis were used for experiments.…”

Section: Methodsmentioning

confidence: 99%

“…ajor steps toward the commercialization of closed life cycle aquaculture production of the ornate spiny lobster (Panulirus ornatus) have been reported recently (36), yet nutritional deficits (41) and mortality caused by bacterial disease (5) remain major constraints to hatchery productivity. Mass mortalities of larvae (phyllosomas) are often associated with enteric vibriosis, an infection of the midgut gland (hepatopancreas) caused by Vibrio species (6,52).…”

mentioning

confidence: 99%

Pathogenicity and Infection Cycle of Vibrio owensii in Larviculture of the Ornate Spiny Lobster (Panulirus ornatus)

Goulden

Hall

Bourne

et al. 2012

Appl Environ Microbiol

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bThe type strain of Vibrio owensii (DY05) was isolated during an epizootic of aquaculture-reared larvae (phyllosomas) of the ornate spiny lobster (Panulirus ornatus). V. owensii DY05 was formally demonstrated to be the etiological agent of a disease causing rapid and reproducible larval mortality with pathologies similar to those seen during disease epizootics. Vectored challenge via the aquaculture live feed organism Artemia (brine shrimp) caused consistent cumulative mortality rates of 84 to 89% after 72 h, in contrast to variable mortality rates seen after immersion challenge. Histopathological examination of vector-challenged phyllosomas revealed bacterial proliferation in the midgut gland (hepatopancreas) concomitant with epithelial cell necrosis. A fluorescent-protein-labeled V. owensii DY05 transconjugant showed dispersal of single cells in the foregut and hepatopancreas 6 h postexposure, leading to colonization of the entire hepatopancreas within 18 h and eventually systemic infection. V. owensii DY05 is a marine enteropathogen highly virulent to P. ornatus phyllosoma that uses vector-mediated transmission and release from host association to a planktonic existence to perpetuate transfer. This understanding of the infection process will improve targeted biocontrol strategies and enhance the prospects of commercially viable larviculture for this valuable spiny lobster species. Major steps toward the commercialization of closed life cycle aquaculture production of the ornate spiny lobster (Panulirus ornatus) have been reported recently (36), yet nutritional deficits (41) and mortality caused by bacterial disease (5) remain major constraints to hatchery productivity. Mass mortalities of larvae (phyllosomas) are often associated with enteric vibriosis, an infection of the midgut gland (hepatopancreas) caused by Vibrio species (6, 52).Members of the genus Vibrio are natural marine inhabitants, playing important roles in nutrient cycling and forming associations with zooplankton (49). Microhabitat preferences and ecological selection may be key factors in the speciation of vibrios (20), and intensive aquaculture systems are thought to select for bacterial virulence, including traits that enhance infectivity and transmission (29,33). Accordingly, many Vibrio species are pathogenic to cultured crustacean zooplanktonic larval forms, including the three closely related species Vibrio harveyi (32, 35), V. campbellii (17, 44), and the recently described V. owensii (8).It is of paramount importance to the development of efficient disease management strategies that pathogens be identified by using experimental infection models that provide information on infection routes and infection dynamics (38). Recently, microorganisms engineered to express fluorescent proteins (FP) have significantly increased the understanding of invasive pathways and infection dynamics of pathogens, including V. anguillarum, Aeromonas hydrophila, and Edwardsiella tarda in fish models (10, 24, 30) and V. harveyi in abalone (50). Panulirus sp. p...

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Description of the larval morphology of captive reared Panulirus ornatus spiny lobsters, benchmarked against wild-caught specimens

Cited by 43 publications

References 22 publications

Identification of an Antagonistic Probiotic Combination Protecting Ornate Spiny Lobster (Panulirus ornatus) Larvae against Vibrio owensii Infection

Identification of an Antagonistic Probiotic Combination Protecting Ornate Spiny Lobster (Panulirus ornatus) Larvae against Vibrio owensii Infection

The effects of pre-digested protein sources on the performance of early–mid stage Panulirus ornatus phyllosoma

Pathogenicity and Infection Cycle of Vibrio owensii in Larviculture of the Ornate Spiny Lobster (Panulirus ornatus)

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