Description of the Gram-Negative, Obligately Aerobic, Nitrilotriacetate (NTA)-Utilizing Bacteria as Chelatobacter heintzii, gen. nov., sp. nov., and Chelatococcus asaccharovorans, gen. nov., sp. nov.

Auling, Georg; Busse, Hans‐Jürgen; Egli, Thomas; El-Banna, Tarek; Stackebrandt, Erko

doi:10.1016/s0723-2020(11)80254-7

Cited by 80 publications

(62 citation statements)

References 38 publications

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“…The major polar lipids of C. asaccharovorans DSM 6462 T analysed in this study were the same. Major polyamines detected in this study were symhomospermidine, spermidine and putrescine, as also found in C. asaccharovorans (Auling et al, 1993). The DNA G+C content of strain K106 T was 68.3 mol%.…”

supporting

confidence: 76%

“…Strain K106 T differed from C. asaccharovorans in that its cells were rod-shaped. However, there were no distinct phenotypic, particularly chemotaxonomic, properties to differentiate strain K106 T from C. asaccharovorans, as shown by Auling et al (1993) and in this study. Accordingly, it seems reasonable that strain K106 T is classified as a member of the genus Chelatococcus.…”

contrasting

confidence: 61%

“…The genus Chelatococcus was proposed by Auling et al (1993) with Chelatococcus asaccharovorans as the sole recognized species of the genus. Phylogenetic analysis based on 16S rRNA gene sequences showed that the genus Chelatococcus falls within the Alphaproteobacteria (Auling et al, 1993;Lee et al, 2005).…”

mentioning

confidence: 99%

“…Phylogenetic analysis based on 16S rRNA gene sequences showed that the genus Chelatococcus falls within the Alphaproteobacteria (Auling et al, 1993;Lee et al, 2005). In this study, we describe a bacterial strain, K106 T , which was isolated from wastewater of a textile dye works in Korea.…”

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confidence: 99%

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Chelatococcus daeguensis sp. nov., isolated from wastewater of a textile dye works, and emended description of the genus Chelatococcus

Yoon

Kang

et al. 2008

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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Chelatococcus daeguensis sp. nov., isolated from wastewater of a textile dye works, and emended description of the genus Chelatococcus A Gram-negative, non-spore-forming, rod-shaped bacterial strain, K106 T , was isolated from wastewater collected from a textile dye works in Korea. Strain K106 T grew optimally at pH 7.0-7.5 and 30-37 6C in the presence of 0-1.0 % (w/v) NaCl. A phylogenetic tree based on 16S rRNA gene sequences showed that strain K106 T joined the type strain of Chelatococcus asaccharovorans with a bootstrap resampling value of 99.9 %. The predominant ubiquinone of strain K106 T was Q-10. The fatty acid profile of strain K106 T was similar to that of C. asaccharovorans DSM 6462 T . Major polar lipids of strain K106 T and C. asaccharovorans DSM 6462 T were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, two aminolipids and two unidentified phospholipids. sym-Homospermidine, spermidine and putrescine were major polyamines. The DNA G+C content was 68.3 mol%. Strain K106 T exhibited 16S rRNA gene sequence similarity of 96.6 % to the type strain of C. asaccharovorans. DNA-DNA relatedness data and differential phenotypic properties, particularly differences in cell morphology and the ability to utilize nitrilotriacetate, demonstrated that strain K106 T can be differentiated from C. asaccharovorans. On the basis of phenotypic, phylogenetic and genetic data, strain K106 T represents a novel species of the genus Chelatococcus, for which the name Chelatococcus daeguensis sp. nov. is proposed.

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supporting

confidence: 76%

contrasting

confidence: 61%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Chelatococcus daeguensis sp. nov., isolated from wastewater of a textile dye works, and emended description of the genus Chelatococcus

Yoon

Kang

et al. 2008

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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“…The genus currently encompasses a single species with a validly published name, Microvirga subterranea, and is assigned to the class Alphaproteobacteria, phylum Proteobacteria (Garrity & Holt, 2001;Garrity et al, 2005a), almost equidistant from Methylobacterium species (Patt et al, 1976;Urakami et al, 1993), Chelatococcus asaccharovorans (Auling et al, 1993;Egli et al, 1988) and Bosea thiooxidans (Das et al, 1996), its nearest phylogenetic relatives, with a mean similarity value of 93 %.…”

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confidence: 99%

Microvirga guangxiensis sp. nov., a novel alphaproteobacterium from soil, and emended description of the genus Microvirga

Zhang

Song

Xin

et al. 2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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Microvirga guangxiensis sp. nov., a novel alphaproteobacterium from soil, and emended description of the genus Microvirga A Gram-negative-staining bacterium, designated strain 25B T , was isolated from a soil sample from a rice field in Guangxi Province, China, and its taxonomic position was investigated by using a polyphasic approach. Cells were rod-shaped, non-spore-forming, non-motile and strictly aerobic. Strain 25B T grew optimally at 37 6C and pH 7.0. The predominant fatty acids of this soil isolate were C 18 : 1 v7c, C 19 : 0 cyclo v8c and C 16 : 0 . Phylogenetic analysis based on the almostcomplete 16S rRNA gene sequence showed that strain 25B T formed a monophyletic clade with the type strain of Microvirga subterranea; the two organisms shared 97.2 % 16S rRNA gene sequence similarity. However, the two strains shared low DNA-DNA relatedness. Strain 25B T was also readily distinguishable from Microvirga subterranea DSM 14364 T by various phenotypic characteristics. The combination of genotypic and phenotypic data suggests that the isolate represents a novel species of the genus Microvirga, for which the name Microvirga guangxiensis sp. nov. is proposed. The type strain is 25B T (5CGMCC 1.7666 T 5JCM 15710 T ).The genus Microvirga was established by Kanso & Patel (2003) to accommodate strictly aerobic, Gram-negative, non-sporulating, motile and rod-shaped bacteria from free-flowing geothermal waters of a bore tapping the Great Artesian Basin of Australia, which grew optimally at 41 u C and pH 7.0 and had an absolute requirement for yeast extract. The genus currently encompasses a single species with a validly published name, Microvirga subterranea, and is assigned to the class Alphaproteobacteria, phylum Proteobacteria (Garrity & Holt, 2001;Garrity et al., 2005a), almost equidistant from Methylobacterium species (Patt et al., 1976;Urakami et al., 1993) Egli et al., 1988) and Bosea thiooxidans (Das et al., 1996), its nearest phylogenetic relatives, with a mean similarity value of 93 %., Chelatococcus asaccharovorans (AulingA sampling campaign during an ecological survey of soil bacteria in China led to the isolation of a pink-pigmented bacterium, designated strain 25B T . The soil sample (10 cm in depth, pH 6.2) was collected from a rice field in Guangxi Province. A glucose-yeast extract-malt extract (GYM) agar plate (comprising, l 21 : 4 g D-glucose, 4 g yeast extract, 10 g malt extract, 15 g agar; pH 7.3) was inoculated with a suspension of the soil and incubated at 37 u C for 7 days. The isolate that was obtained, strain 25B T , was maintained on LB agar (l 21 : 10 g tryptone, 5 g yeast extract, 10 g NaCl, 15 g agar; pH 7.0) at 4 u C and as glycerol suspensions (20 %, v/v) at 220 u C. Biomass for the chemotaxonomic and molecular systematic studies was prepared as described previously (Zhang et al., 2002) with the modification that the strain was grown in shake flasks of LB broth.Micromorphological and colonial properties of strain 25B T were examined on LB agar, Rourf's agar (Mulder & Deinema, 1992) and ...

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Chelatococcus

Egli¹,

Auling²

2015

Bergey's Manual of Systematics of Archaea and Bacteria

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Che.la' to.coc.cus . Gr. n. chele claw; M.L. v. chelato to form claw‐like complexes with divalent cations, i.e., to chelate; Gr. n. coccus berry, used for a bacterium of roughly spherical shape; M.L. masc. n. Chelatococcus chelating coccus. Proteobacteria / Alphaproteobacteria / Rhizobiales / Beijerinckiaceae / Chelatococcus Short , stout , almost coccoid rods , 0 . 5–1 × 1–2 μ m ; resemble diplococci during exponential growth . Gram negative. Nonmotile . Obligately aerobic . Optimal growth temperature, 35–37°C; temperature range for growth, 4–41°C. Slow growth with all substrates tested so far (μ max <0.2 h −1 ). Poly‐β‐hydroxybutyrate is accumulated within the cells. Utilize the metal‐chelating aminopolycarboxylic acids nitrilotriacetic acid ( NTA ) and S , S ‐ ethylenediaminedisuccinic acid as sole sources of carbon , energy , and nitrogen . Generally, sugars are not utilized (except for glycerol). Require one or more vitamins . All strains are sensitive to β‐lactam antibiotics but resistant to nalidixic acid. Ubiquinone Q‐10 is present. The main polyamine present is sym ‐homospermidine; putrescine and spermidine occur as major polyamines, and spermine as a minor polyamine. The mol % G + C of the DNA is : 63. Type species : Chelatococcus asaccharovorans Auling, Busse, Egli, El‐Banna and Stackebrandt 1993b, 624 (Effective publication: Auling, Busse, Egli, El‐Banna and Stackebrandt 1993a, 110.)

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Description of the Gram-Negative, Obligately Aerobic, Nitrilotriacetate (NTA)-Utilizing Bacteria as Chelatobacter heintzii, gen. nov., sp. nov., and Chelatococcus asaccharovorans, gen. nov., sp. nov.

Cited by 80 publications

References 38 publications

Chelatococcus daeguensis sp. nov., isolated from wastewater of a textile dye works, and emended description of the genus Chelatococcus

Chelatococcus daeguensis sp. nov., isolated from wastewater of a textile dye works, and emended description of the genus Chelatococcus

Microvirga guangxiensis sp. nov., a novel alphaproteobacterium from soil, and emended description of the genus Microvirga

Chelatococcus

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