Description of somatic-embryo-forming single cells in carrot suspension cultures employing video cell tracking

Toonen, M.A.J.; Hendriks, Théo; Schmidt, E.D.L.; Verhoeven, H. A.; Kammen, A. van; Vries, S.C. de

doi:10.1007/bf00714471

Cited by 108 publications

(54 citation statements)

References 23 publications

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“…The cell population passing through the 50 ~m sieve was either isolated as <50 t~m population or transferred to the 30 lim sieve. Single cells and cell clusters passing this last sieve are designated as <30 I~m populations (Toonen et al, 1994).…”

Section: Plant Material Gene Transfer and Cell Culturementioning

confidence: 99%

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AtLTP1 luciferase expression during carrot somatic embryogenesis

et al. 1997

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SummaryThe carrot (Daucus carota L.) EP2 gene encodes a Lipid Transfer Protein (LTP) which is expressed during protoderm formation in developing embryos. To develop a vital reporter system for gene expression during somatic embryo development a 1.1 kB fragment of the Arabidopsis thaliana LTP1 promoter was fused to the firefly luciferase (LUC) coding sequence. The AtLTP1 luciferase expression pattern in transformed carrot suspension cultures was identical to the expression pattern of the endogenous carrot EP2 gene. Cell tracking experiments revealed that all somatic embryos were derived from AtLTP1 luciferase expressing cell clusters, However, not all cell clusters that expressed the AtLTP1 luciferase reporter gene developed into a somatic embryo, suggesting that initiation of an embryogenic pathway in tissue culture does not always lead to development of a somatic embryo.

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Section: Plant Material Gene Transfer and Cell Culturementioning

confidence: 99%

“…The final luciferin concentration in the culture was 0.02 I~M. After 7 days of culture 2,4-D and luciferin was removed from the cultures by washing as described previously (Toonen et al, 1994). The 50-125 population was embedded in a similar way at 40 000 cells per dish in P1/B5 medium without 2,4-D.…”

Section: Cell Immobilizationmentioning

confidence: 99%

AtLTP1 luciferase expression during carrot somatic embryogenesis

et al. 1997

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“…Komamine (1985, 1995) have shown that isolated, small, cytoplasm-rich carrot cells have the ability to develop into somatic embryos. In carrot cultures, several phenotypes of cells capable for SE (embryogenic) were described (Toonen et al, 1994) but the efficiency of SE was highest in cells with a small size, a rich cytoplasm and which are spherical. The comparison of the embryogenic and non-embryogenic parts of explants is much easier as the non-embryogenic parts of explants are highly vacuolated (Fig.…”

Section: Meristematic and Embryogenic Cells Within Explantsmentioning

confidence: 99%

Cellular Markers for Somatic Embryogenesis

Kurczyńska¹,

Potocka²,

Dobrowolska³

et al. 2012

Embryogenesis

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“…Next to that, adding preconditioned medium obtained from an embryogenic culture [28] or co-cultivation with zygotic embryos [70] also increases the embryogenic potential of embryogenic cultures. Labeling of cells with the JIM8 antibody, which recognizes a certain arabinogalactan protein (AGP) epitope present in embryogenic cultures, is indicative for the EC of a cell line [144,202]. Cells labeled with JIM8 were believed to be in a transition towards an embryogenic state because a subpopulation of small cytoplasm rich cells in an embryogenic culture was recognized by JIM8 [144].…”

Section: Cell-cell Communicationmentioning

confidence: 99%

“…Cells labeled with JIM8 were believed to be in a transition towards an embryogenic state because a subpopulation of small cytoplasm rich cells in an embryogenic culture was recognized by JIM8 [144]. Cell tracking of the JIM8-labeled cells, however, did not reveal a relationship between embryogenesis and JIM-8 labeling, suggesting that the JIM8-labeled cells perform an accessory function in SE [202]. The finding that in the absence of the JIM8-labeled cell population, no somatic embryos develop, but callus is formed, demonstrates the importance of cell-cell communication in the maintenance of EC in culture [145].…”

Section: Cell-cell Communicationmentioning

confidence: 99%

Molecular aspects of somatic-to-embryogenic transition in plants

2009

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Somatic embryogenesis (SE) is a model system for understanding the physiological, biochemical, and molecular biological events occurring during plant embryo development. Plant somatic cells have the ability to undergo sustained divisions and give rise to an entire organism. This remarkable feature is called plant cell totipotency. SE is a notable illustration of plant totipotency and involves reprogramming of development in somatic cells toward the embryogenic pathway. Plant growth regularities, especially auxins, are key components as their exogenous application recapitulates the embryogenic potential of the mitotically quiescent somatic cells. It has been observed that there are genetic and also physiological factors that trigger in vitro embryogenesis in various types of plant somatic cells. Analysis of the proteome and transcriptome has led to the identification and characterization of certain genes involved in SE. Most of these genes, however, are upregulated only in the late developmental stages, suggesting that they do not play a direct role in the vegetative-to-embryogenic transition. However, the molecular bases of those triggering factors and the genetic and biochemical mechanisms leading to in vitro embryogenesis are still unknown. Here, we describe the plant factors that participate in the vegetative-to-embryogenic transition and discuss their possible roles in this process.

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Description of somatic-embryo-forming single cells in carrot suspension cultures employing video cell tracking

Cited by 108 publications

References 23 publications

AtLTP1 luciferase expression during carrot somatic embryogenesis

AtLTP1 luciferase expression during carrot somatic embryogenesis

Cellular Markers for Somatic Embryogenesis

Molecular aspects of somatic-to-embryogenic transition in plants

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