Cellular Markers for Somatic Embryogenesis

Kurczyńska, Ewa; Potocka, Izabela; Dobrowolska, Izabela; Kulińska-Łukaszek, Katarzyna; Sala, Katarzyna; Wrobel, Justyna

doi:10.5772/37617

Cited by 4 publications

(3 citation statements)

References 105 publications

(104 reference statements)

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“…From that moment, we have seen many results that confirm the regulation of symplasmic communication by callose, including results indicating its significant SE regulation. An analysis of the callose deposition during the SE of Arabidopsis showed that cells that changed the direction of differentiation were isolated by callose [ 102 ].…”

Section: Symplasmic Isolation As a Mechanism Controlling Somatic Embr...mentioning

confidence: 99%

Apoplastic and Symplasmic Markers of Somatic Embryogenesis

Kurczyńska

Godel-Jędrychowska

2023

Plants

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Somatic embryogenesis (SE) is a process that scientists have been trying to understand for many years because, on the one hand, it is a manifestation of the totipotency of plant cells, so it enables the study of the mechanisms regulating this process, and, on the other hand, it is an important method of plant propagation. Using SE in basic research and in practice is invaluable. This article describes the latest, but also historical, information on changes in the chemical composition of the cell wall during the transition of cells from the somatic to embryogenic state, and the importance of symplasmic communication during SE. Among wall chemical components, different pectic, AGP, extensin epitopes, and lipid transfer proteins have been discussed as potential apoplastic markers of explant cells during the acquisition of embryogenic competence. The role of symplasmic communication/isolation during SE has also been discussed, paying particular attention to the formation of symplasmic domains within and between cells that carry out different developmental processes. Information about the number and functionality of plasmodesmata (PD) and callose deposition as the main player in symplasmic isolation has also been presented.

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Section: Symplasmic Isolation As a Mechanism Controlling Somatic Embr...mentioning

confidence: 99%

Apoplastic and Symplasmic Markers of Somatic Embryogenesis

Kurczyńska

Godel-Jędrychowska

2023

Plants

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“…Changes in cell fate and the direction of differentiation during plant regeneration rely on the intracellular embryogenic developmental program and physiological regulation (Kurczynska et al ., 2012). Additionally, intercellular permeability is important for the exchange of information between plant cells (Scheres, 2001), which relies on the presence of specialized structures plasmodesmata (PD) known as the organelle link the cytoplasm of plant neighboring cells (Romberger et al ., 2004).…”

Section: Introductionmentioning

confidence: 99%

“…Additionally, intercellular permeability is important for the exchange of information between plant cells (Scheres, 2001), which relies on the presence of specialized structures plasmodesmata (PD) known as the organelle link the cytoplasm of plant neighboring cells (Romberger et al ., 2004). They play effective roles in participating in the regulation and coordination of plant development (Lucas & Lee, 2004; Kurczynska et al ., 2012; Sevilem et al ., 2015). The presence of PD within callus tissue implied the potential importance during SE (Pina et al ., 2009; Kamila et al ., 2020).…”

Section: Introductionmentioning

confidence: 99%

Organelle Ca²⁺/CAM1‐SELTP confers somatic cell embryogenic competence acquisition and transformation in plant regeneration

Guo,

Zhang

et al. 2024

New Phytologist

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Summary Somatic cell totipotency in plant regeneration represents the forefront of the compelling scientific puzzles and one of the most challenging problems in biology. How somatic embryogenic competence is achieved in regeneration remains elusive. Here, we discover uncharacterized organelle‐based embryogenic differentiation processes of intracellular acquisition and intercellular transformation, and demonstrate the underlying regulatory system of somatic embryogenesis‐associated lipid transfer protein (SELTP) and its interactor calmodulin1 (CAM1) in cotton as the pioneer crop for biotechnology application. The synergistic CAM1 and SELTP exhibit consistent dynamical amyloplast–plasmodesmata (PD) localization patterns but show opposite functional effects. CAM1 inhibits the effect of SELTP to regulate embryogenic differentiation for plant regeneration. It is noteworthy that callus grafting assay reflects intercellular trafficking of CAM1 through PD for embryogenic transformation. This work originally provides insight into the mechanisms responsible for embryogenic competence acquisition and transformation mediated by the Ca2+/CAM1‐SELTP regulatory pathway, suggesting a principle for plant regeneration and cell/genetic engineering.

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High expression of SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE coincides with initiation of various developmental pathways in in vitro culture of Trifolium nigrescens

et al. 2015

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The aim of this study was to identify and examine the expression pattern of the ortholog of SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE gene from Trifolium nigrescens (TnSERK) in embryogenic and non-regenerative cultures of immature cotyledonary-stage zygotic embryos (CsZEs). In the presence of 1-naphthaleneacetic acid and N6-[2-isopentenyl]-adenine, the CsZE regenerated embryoids directly and in a lengthy culture produced callus which was embryogenic or remained non-regenerative. As revealed by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR), the TnSERK was expressed in both embryogenic and non-regenerative cultures, but the expression level was significantly higher in embryogenic ones. An in situ RNA hybridization assay revealed that the expression of TnSERK preceded the induction of cell division in explants, and then, it was maintained exclusively in actively dividing cells from which embryoids, embryo-like structures (ELSs), callus or tracheary elements were produced. However, the cells involved in different morphogenic events differed in intensity of hybridization signal which was the highest in embryogenic cells. The TnSERK was up-regulated during the development of embryoids, but in cotyledonary embryos, it was preferentially expressed in the regions of the apical meristems. The occurrence of morphological and anatomical abnormalities in embryoid development was preceded by a decline in TnSERK expression, and this coincided with the parenchymatization of the ground tissue in developing ELSs. TnSERK was also down-regulated during the maturation of parenchyma and xylem elements in CsZE and callus. Altogether, these data suggest the involvement of TnSERK in the induction of various developmental programs related to differentiation/transdifferentiation and totipotent state of cell(s).Electronic supplementary materialThe online version of this article (doi:10.1007/s00709-015-0814-5) contains supplementary material, which is available to authorized users.

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Cellular Markers for Somatic Embryogenesis

Cited by 4 publications

References 105 publications

Apoplastic and Symplasmic Markers of Somatic Embryogenesis

Apoplastic and Symplasmic Markers of Somatic Embryogenesis

Organelle Ca²⁺/CAM1‐SELTP confers somatic cell embryogenic competence acquisition and transformation in plant regeneration

High expression of SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE coincides with initiation of various developmental pathways in in vitro culture of Trifolium nigrescens

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Cellular Markers for Somatic Embryogenesis

Cited by 4 publications

References 105 publications

Apoplastic and Symplasmic Markers of Somatic Embryogenesis

Apoplastic and Symplasmic Markers of Somatic Embryogenesis

Organelle Ca2+/CAM1‐SELTP confers somatic cell embryogenic competence acquisition and transformation in plant regeneration

High expression of SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE coincides with initiation of various developmental pathways in in vitro culture of Trifolium nigrescens

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Organelle Ca²⁺/CAM1‐SELTP confers somatic cell embryogenic competence acquisition and transformation in plant regeneration