Description of Ruehmaphelenchus thailandae n. sp. (Nematoda: Aphelenchoididae) found in dunnage from Thailand

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Bursaphelenchus arthuroides sp. n. is described and figured from pine packaging wood originating in Brazil, and inspected in Ningbo harbour, P. R. China. The new species clearly belongs to the fungivorus group. It is characterised by four lines in the lateral field; spicules medium sized (15-17 μm), dorsal part distinctly sclerotised, with a high rounded condylus and a blunted pointed rostrum in the middle position, capitulum with a shallow depression, the posterior third of the dorsal limb ventrally bent, distal end broadly rounded without cucullus; the shape of the female tail conical and slim, posterior third distinctly ventrally bent with a finely pointed terminus; vulval lips slightly protruding but not forming a vulval flap and post-uterine sac extending for about half the vulva-anus distance. Species status is supported by ITS-RFLP patterns and molecular phylogenetic analysis based on partial LSU sequences, ITS1/2 and partial LSU sequences, which revealed that B. arthuroides sp. n. is closest to B. arthuri. Propagation tests also confirmed that the new species is parthenogenetic.

Section: Molecular Profiles and Phylogenetic Statusmentioning

confidence: 74%

Section: Diagnosis and Relationshipsmentioning

confidence: 99%

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Description of Bursaphelenchus arthuroides sp. n. (Nematoda: Aphelenchoididae), a second parthenogenetic species of Bursaphelenchus Fuchs, 1937

Wang

Zheng

2012

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“…The primers and reaction conditions followed the method developed by Vrain (1993). PCR products were purified for cloning and sequenced by Invitrogen (Gu & Wang, 2010).…”

Section: Molecular Analysesmentioning

confidence: 99%

Deladenus prorsus n. sp. (Nematoda: Neotylenchidae) in dunnage wood from Malaysia intercepted in Ningbo, China

2013

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Deladenus prorsus n. sp., isolated from dunnage wood originating from Malaysia and intercepted in Ningbo port, P.R. China, is described and illustrated. The dunnage wood material appeared to have holes resembling the typical exit holes of wood-boring insects and have associated blue stain. The new species is characterised by the presence of both the mycophagous and infective forms {Deladenus consists of mycetophagous-only forms and those with known female dimorphism), the body length of 814 (670-1147) and 898 (812-979) /xm for mycetophagous males and females, respectively, and 1129 (1114-1352) fj.m for the infective female, and by the very anterior position of the excretory pore which is situated at 30.7 (24.4-33.0), 30.6 (25.4-35.6) and 37.9 (27.3-39.7) ßm for the mycetophagous males and females and infective females, respectively, and the distance that the excretory pore is located anterior to the hemizonid which is 82 (77-90), 74 (54-92) and 86 (64-106) /xm for the mycetophagous males and females and infective females, respectively. The diagnostic value of the excretory pore and the hemizonid of the species of the genus are discussed. The ribosomal DNA ITS region was sequenced and analysed.

“…In addition, the genus is sometimes included in the Aphelenchoidinae clade by phylogenetic analyses based on sequences of internal transcribed spacer (ITS) regions (Braasch et ai, 2006;Gu & Wang, 2010;Gu et al, 2012). Additional phylogenetic, genomic and morpholo-• * Corresponding author, e-mail: nkanzaki@affrc.go.jp gical studies are needed to determine the systematic and taxonomic status of the genus.…”

mentioning

confidence: 99%

Description of Ruehmaphelenchus formosanus n. sp. (Tylenchina: Aphelenchoididae) isolated from Euwallacea fornicates from Taiwan

Kanzaki

Masuya

Taki

et al. 2013

A Ruehmaphelenchus species was isolated from an ambrosia beetle, Euwallacea fornicates, during a biodiversity survey of entomophilic neinatodes in Taichung, Taiwan. The new species is characterised by a unique tail morphology in both males and females, and a unique spicule morphology in males. The male spieule has clear dorsal and ventral limbs (connected by a blade-like cuticle), a triangular membrane-like structure on its sides, and short, conspicuous, laterally oriented, projections at the distal end. In a molecular phylogenetic tree, inferred from near-full-length small subunit (SSU: 18S) and D2/D3 expansion segments ofthe large subunit (LSU: 28S) of ribosomal RNA, the new species and other nominal and undescribed Ruehmaphelenchus species formed a well supported clade within Bursaphelenchus. Although this result supports a previous study that suggested that Ruehmapheienchus is a junior synonym of Bursapheienchus, the generic relationship between Ruehniaphelenchiis and Bursaphelenchus remains somewhat uncertain. Therefore, R. formosanus n. sp. is deseribed as a member oí Ruehmaphelenchus, although this should be regarded as a tentative placement.