Deladenus prorsus n. sp. (Nematoda: Neotylenchidae) in dunnage wood from Malaysia intercepted in Ningbo, China

Abstract

Deladenus prorsus n. sp., isolated from dunnage wood originating from Malaysia and intercepted in Ningbo port, P.R. China, is described and illustrated. The dunnage wood material appeared to have holes resembling the typical exit holes of wood-boring insects and have associated blue stain. The new species is characterised by the presence of both the mycophagous and infective forms {Deladenus consists of mycetophagous-only forms and those with known female dimorphism), the body length of 814 (670-1147) and 898 (812-979) /xm for mycetophagous males and females, respectively, and 1129 (1114-1352) fj.m for the infective female, and by the very anterior position of the excretory pore which is situated at 30.7 (24.4-33.0), 30.6 (25.4-35.6) and 37.9 (27.3-39.7) ßm for the mycetophagous males and females and infective females, respectively, and the distance that the excretory pore is located anterior to the hemizonid which is 82 (77-90), 74 (54-92) and 86 (64-106) /xm for the mycetophagous males and females and infective females, respectively. The diagnostic value of the excretory pore and the hemizonid of the species of the genus are discussed. The ribosomal DNA ITS region was sequenced and analysed.

Morphological and Molecular Characterization of Gracilacus wuae n. sp. (Nematoda: Criconematoidea) Associated with Cow Parsnip (Heracleum maximum) in Ontario, Canada

“…Morphological study: The method used was as described previously (Yu et al, 2014). The nematodes were all fixed in TAF and mounted in dehydrated glycerin on slides for morphological studies.…”

Characterisation of the dimorphic Deladenus beddingi n. sp. and its associated woodwasp and fungus

“…By contrast, this feature overlaps between D. beddingi n. sp. and D. siricidicola, D. canii and D. valveus Yu, Popovic & Gu, 2014. The distance between the excretory pore and hemizonid in mycophagous females of D. nitobei is marginally shorter than in D. beddingi n. sp.…”

“…Whilst several new species of dimorphic Deladenus nematodes have been described recently (Nasira et al, 2013;Yu et al, 2013Yu et al, , 2014Kanzaki et al, 2016), no new species thought to be a North American native has been reported or described from North America for several decades (Bedding & Akhurst, 1978;Yu et al, 2014). In general, species of Deladenus associated with the siricids found in western USA are poorly known.…”

“…to be most closely related to two isolates, S. cyaneus 'cya 2' and S. nitidus 'nit30', from the eastern USA. Unfortunately, molecular data are only available for a limited number of Deladenus spp: D. siricidicola (Yu et al, 2009;Mlonyeni et al, 2011;Leal et al, 2012;Morris et al, 2013), D. proximus (Yu et al, 2011;Morris et al, 2013;Zieman et al, 2015;Hartshorn et al, 2017), and D. prorsus Yu, Gu & Ye, 2013(Yu et al, 2013, hampering inclusion of other described species in our analysis. Molecular data have proven to be increasingly important for discriminating these nematode species.…”

Deladenus posteroporus n. sp. (Nematoda: Neotylenchidae) Isolated from Packaging Wood from Canada and White Pine (Pinus monticola) Lumber from the United States and Intercepted in Ningbo, China

“…Several species, including D. lonchites Massey, 1974;D. ruehmi Andrassy, 1958;and D. saccatus Andrassy, 1954, have been listed as species inquirendae (Chitambar, 1991;Siddiqi, 2000;Yu et al, 2014). Recently, six additional species have been described: D. cocophilus Nasira, Shahina and Firoza, 2013;D.…”