Description of novel species of Aliinostoc, Desikacharya and Desmonostoc using a polyphasic approach

Kabirnataj, Sara; Nematzadeh, Ghorbanali; Talebi, Ahmad Farhad; Saraf, Aniket; Suradkar, Archana; Tabatabaei, Meisam; Singh, Prashant

doi:10.1099/ijsem.0.004188

Cited by 29 publications

(35 citation statements)

References 27 publications

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“…The 16S rRNA gene sequence of the A. circularis NIES-21 (accession numbers AF247595 and AP018174), the only Anabaenopsis strain with genome sequenced, fitted within the Desikacharya clade with strong support (100 %) (). Desikacharya is a genus recently created and it was erected with members morphologically similar to the traditional and polyphyletic genus Nostoc from the family Nostocaceae [53, 54]. These authors also recommended that several existing members of Nostoc -like shall be reclassified into this proposed new genus.…”

Section: Resultsmentioning

confidence: 99%

“…2). Desikacharya is a genus recently created and it was erected with members morphologically similar to the traditional and polyphyletic genus Nostoc from the family Nostocaceae [53,54]. These authors also recommended that several existing members of Nostoc-like shall be reclassified into this proposed new genus.…”

Section: S Rrna Gene Phylogeny and 16s-23s Its Secondary Structurementioning

confidence: 99%

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Phylogenomic analysis of Anabaenopsis elenkinii (Nostocales, Cyanobacteria)

Delbaje

Andreote

Pellegrinetti

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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The saline-alkaline lakes (soda lakes) are the habitat of the haloalkaliphilic cyanobacterium Anabaenopsis elenkinii, the type species of this genus. To obtain robust phylogeny of this type species, we have generated whole-genome sequencing of the bloom-forming Anabaenopsis elenkinii strain CCIBt3563 isolated from a Brazilian soda lake. This strain presents the typical morphology of A. elenkinii with short and curved trichomes with apical heterocytes established after separation of paired intercalary heterocytes and also regarding to cell dimensions. Its genome size is 4 495 068 bp, with a G+C content of 41.98 %, a total of 3932 potential protein coding genes and four 16S rRNA genes. Phylogenomic tree inferred by RAxML based on the alignment of 120 conserved proteins using GTDB-Tk grouped A. elenkinii CCIBt3563 together with other genera of the family Aphanizomenonaceae. However, the only previous available genome of Anabaenopsis circularis NIES-21 was distantly positioned within a clade of Desikacharya strains, a genus from the family Nostocaceae. Furthermore, average nucleotide identity values from 86–98 % were obtained among NIES-21 and Desikacharya genomes, while this value was 76.04 % between NIES-21 and the CCIBt3563 genome. These findings were also corroborated by the phylogenetic tree of 16S rRNA gene sequences, which also showed a strongly supported subcluster of A. elenkinii strains from Brazilian, Mexican and Kenyan soda lakes. This study presents the phylogenomics and genome-scale analyses of an Anabaenopsis elenkinii strain, improving molecular basis for demarcation of this species and framework for the classification of cyanobacteria based on the polyphasic approach.

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Section: Resultsmentioning

confidence: 99%

Section: S Rrna Gene Phylogeny and 16s-23s Its Secondary Structurementioning

confidence: 99%

Phylogenomic analysis of Anabaenopsis elenkinii (Nostocales, Cyanobacteria)

Delbaje

Andreote

Pellegrinetti

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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“…Up to now, four species, Desikacharya nostocoides (the type species of Desikacharya), Desikacharya soli, Desikacharya thermotolerans and Desikacharya constricta have been reported under the genus Desikacharya, which are considered certain features such as constrictions along with prominent coiling (Kabirnataj et al 2020). Kabirnataj et al (2020) discussed that Minunostoc cylindricum should be a member of the genus Desikacharya, in contrast, the Desikacharya species within the lineage, were differentiated into two distinct clades with high genetic divergences (less than 95% similarity of 16S rRNA gene), and Desikacharya has been shown to be polyphyletic in our analyses. The 16S rRNA gene sequence of Desikacharya constricta had less than 95% similarities to the type species Desikacharya nostocoides and Desikacharya soli, however, it had 96.9% similarity to Minunostoc cylindricum.…”

Section: Discussionmentioning

confidence: 99%

Description of two new species of Nostoc from China based on the polyphasic approach

Cai¹,

Yu²,

Liu³

et al. 2021

Fottea

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The present study described two new Nostoc species, Nostoc favosum (CHAB5709, CHAB5713, and CHAB5714) and Nostoc mirabile (CHAB5756 and CHAB5784) based on the polyphasic approach combining morphological, genetic and ecological characteristics. Five investigated strains were found to possess morphological features of the Nostoc genus. Results showed that the 16S rRNA gene sequences of these five strains displayed ≥ 95%, and ≤ 98% similarity to the genus Nostoc. The 16S rRNA gene phylogenetic analysis inferred using bayesian inference, maximum-likelihood and neighbour-joining methods placed these five strains on two separate nodes away from other Nostoc species. The 16S-23S rRNA internal transcribed spacer (ITS) secondary structure of two new species showed a unique pattern of D1-D1′, Box-B and V3 helix, which distinguished them from other Nostoc species. And the two species were further established by percent dissimilarity of ITS between another Nostoc species.

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“…Identi cation of the isolate was carried out according to Komárek et al [4]. In addition, recent studies dealing with the description of new Desmonostoc species were considered [7,[10][11][12]14]. A unialgal culture was deposited in the Cyanobacteria Culture Collection (CCC) and dry type material into ALBORZ herbarium.…”

Section: Field Surveysmentioning

confidence: 99%

A Novel Potentially Toxic Cyanobacterial Species From the Genus Desmonostoc, Desmonostoc Alborizicum sp. nov., Iisolated From a Water Supply System of IranA

Nowruzi

Becerra-Absalón

2022

Preprint

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A cyanobacterial mat colonizing the wall of a qanat was found to be responsible for damages affecting the passage of water through the water supply system in Golestan province, Gorgan city, Iran. A qanat or kariz is a slightly sloping underground aqueduct used to transport water from water wells or aquifers to the surface for irrigation and drinking supply. A cyanobacterial strain was isolated from the mat and grown in BG11 liquid medium. Fragments of 16S rRNA, mcyG and mcyD genes were amplified and sequenced, as well as the 16S-23S internal transcribed spacer (ITS), from which secondary structures were analyzed. The obtained molecular data, together with morphological and physiological observations, were used to describe the new organism. The isolate was related to a morphotype of Nostoc sensu lato group, with similar characteristics of Desmonostoc, after microscopic inspections. The 16S rRNA phylogenetic analysis placed the isolate into the typical cluster of the recently proposed genus Desmonostoc. Morphological analysis revealed distinctive characteristic and secondary structures of 16S–23S rRNA (D1-D1´ and Box-B regions) derived from comparative analysis, which did not match known species of Desmonostoc. These results lead us to propose a novel Desmonostoc species, Desmonostoc alborizicum, which was described and compared with similar taxa. Furthermore, for the first time a potential toxic species of Desmonostoc was isolated from water supply, since the mcyD and mcyG genes of microcystin synthetase (mcy) cluster was successfully sequenced. The presence of the microcystin was confirmed by chemical analyses that also lead to the discovery of numerous other cyanobacterial secondary metabolites. We recognized substantial amounts of the potent microcystin variant microcystin-LR present in cell extracts of the Desmonostoc strain. Our findings contribute to a deeper understanding of diversity, systematics, and occurrence of Desmonostoc genus.

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Description of novel species of Aliinostoc, Desikacharya and Desmonostoc using a polyphasic approach

Abstract: Five cyanobacterial strains with Nostoc -like morphology from different localities of the Mazandaran province of Iran were characterized using a polyphasic approach. Three st… Show more

Cited by 29 publications

References 27 publications

Phylogenomic analysis of Anabaenopsis elenkinii (Nostocales, Cyanobacteria)

Phylogenomic analysis of Anabaenopsis elenkinii (Nostocales, Cyanobacteria)

Description of two new species of Nostoc from China based on the polyphasic approach

A Novel Potentially Toxic Cyanobacterial Species From the Genus Desmonostoc, Desmonostoc Alborizicum sp. nov., Iisolated From a Water Supply System of IranA

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