Description of microbial diversity associated with ticks Hyalomma dromedarii (Acari: Ixodidae) isolated from camels in Hail region (Saudi Arabia) using massive sequencing of 16S rDNA

Alreshidi, Mousa; Veettil, Vajid N.; Noumi, Emira; Campo, R. del

doi:10.6026/97320630016602

Cited by 7 publications

(10 citation statements)

References 39 publications

(28 reference statements)

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“…Camels were heavily infested (94.3%) with H. dromedarii compared to all animals and these results concurred with our previous studies in the UAE [12,39]. Hyalomma dromedarii is known to harbor a variety of microbes with some tick-borne bacterial [25,[40][41][42] and protozoan pathogens [25] in the MENA region, including the UAE. There was no difference in tick prevalence between male and female hosts with the exception of goats, where more females were infested compared to males.…”

Section: Discussionsupporting

confidence: 90%

Prevalence, Distribution, and Molecular Record of Four Hard Ticks from Livestock in the United Arab Emirates

Perveen

Muzaffar

Al-Deeb

2021

Insects

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Ticks are important arthropod vectors that serve as reservoirs of pathogens. Rapid urbanization and changes in animal breeding practices could be causing a rise in tick burden on animals. Studies on tick distribution on livestock and tick molecular diversity from the United Arab Emirates (UAE) are limited. The aim of this study was to (i) provide molecular and morphological identification of tick species, (ii) compare tick infestation between different hosts, (iii) compare tick infestation in relation to the sex of the host, and (iv) assess the prevalence of tick species on hosts. A total of 5950 ticks were collected from camels (4803 ticks), cows (651 ticks), goats (219 ticks), and sheep (277 ticks). Ticks were identified based on morphological characters at the species level using taxonomic keys. In addition, Polymerase Chain Reaction (PCR) amplification of the cytochrome oxidase subunit 1 (cox1) and 16S rRNA mitochondrial genes was used to identify ticks. Four species were confirmed based on molecular and morphological characterization, namely, Hyalomma dromedarii, Hyalomma anatolicum, Rhipicephalus sanguineus, and Amblyomma lepidum. Hyalomma dromedarii (94.3%) was the most abundant species, followed by H. anatolicum (32.8%). Camels were heavily infested (94%) with ticks as compared to cows (38%), sheep (37%), and goats (14%). Widespread occurrence of these four tick species in the UAE poses a risk of spreading tick-borne pathogens wherever the conditions of infection prevail.

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Section: Discussionsupporting

confidence: 90%

Prevalence, Distribution, and Molecular Record of Four Hard Ticks from Livestock in the United Arab Emirates

Perveen

Muzaffar

Al-Deeb

2021

Insects

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“…Firmicutes, Proteobacteria , and Actinobacteria were the abundant phyla found in the present dataset and notably, Firmicutes was the most abundant one in all months. These results were slightly different from previous studies that reported all three phyla; however, they found that Proteobacteria was the most abundant phylum ( 22 , 33 , 34 ).…”

Section: Discussioncontrasting

confidence: 99%

“…Microbial infections are pervasive in animal and human populations in healthy ecosystems ( 32 ). A limited number of studies on the microbial community structure of H. dromedarii ( 22 , 33 – 35 ) pointed to complex microbial assemblages in dwelling ticks comprising endosymbionts, commensals, and pathogens. Because in the UAE H. dromedarii ticks exist in the desert ecosystem under harsh environmental conditions, especially the extreme summer heat and drought, we hypothesize that their microbial species composition has gone way off in terms of the types of typical taxa found in other tick species or H. dromedarii in temperate environments.…”

Section: Introductionmentioning

confidence: 99%

Assessing Temporal Changes in Microbial Communities in Hyalomma dromedarii Collected From Camels in the UAE Using High-Throughput Sequencing

et al. 2022

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Ticks (Acari) are ectoparasites of animals that harbor communities of microbes of importance to animal and human health. Microbial communities associated with ticks exhibit temporal patterns of variation in their composition, with different genera dominating at different times of the year. In this study, molecular tools were used to assess the composition of the microbial communities associated with Hyalomma dromdarii. Adult ticks were collected every month for 1 year from 25 camels in the UAE. A total of 12 DNA pools were prepared (one pool for each month). We monitored the microbiota of ticks using high-throughput sequencing of the V3–V4 region of the bacterial 16S rRNA gene. A total of 614 operational taxonomic units were produced through de novo clustering and belonged to 17 phyla, 30 classes, 46 orders, 118 families, and 222 genera. Fifteen bacterial families were found to be the most abundant. The dominant bacterial communities associated with H. dromedarii belonged to the genera Staphylococcus, Bacillus, Francisella, and Corynebacterium, which were reported with high relative abundance from all months. No significant correlation occurred between the abundance of microbial families or genera in H. dromedarii ticks and the ambient temperature. Our findings revealed, for the first time in the UAE, temporal fluctuations of microbial communities in H. dromedarii ticks and provided key insights on the interaction between different microbial groups. Moreover, our results contribute to the current understanding of disease development and allow more investigations for potentially pathogenic microbiota.

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“…However, in the UAE, the genus Francisella was previously reported in H. dromedarii collected from camels, with a high relative abundance of 99.1% [ 8 ]. In addition, almost similar results were reported from Saudi Arabia, where Francisella was found to be a dominant genus with an abundance of 94.4% [ 34 ] and 42.1% [ 35 ] in H. dromedarii . It has been shown that tick endosymbionts mostly belong to the genera Francisella, Rickettsia and Coxiella [ 36 ] and that they probably have mutualistic relationships with ticks possibly also modulate tick vector capacity by influencing tick-borne pathogens’ colonization and transmission to vertebrate hosts [ 9 , 37 ].…”

Section: Discussionsupporting

confidence: 82%

Microbial composition in Hyalomma anatolicum collected from livestock in the United Arab Emirates using next-generation sequencing

et al. 2022

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Background Hyalomma anatolicum is a widely distributed tick species that acts as a vector transmitting tick-borne pathogens (TBPs) in livestock. Such pathogens affect the health of livestock and consequently reduce their productivity. Knowledge about the microbial communities (pathogens and endosymbionts) of ticks in the United Arab Emirates (UAE) is scarce. Therefore, the aim of the present study was to quantify microbial diversity in H. anatolicum using next-generation sequencing (NGS) technology. Methods Hyalomma anatolicum ticks were collected from livestock in the emirates of Abu Dhabi, Dubai and Sharjah in the UAE during 2019. DNA was extracted from 175 male ticks sampled from livestock (n = 78) and subjected to NGS. The 16S rRNA gene was analyzed using the Illumina MiSeq platform to determine the bacterial communities. Principal coordinates analysis (PCA) was performed to identify patterns of diversity in the bacterial communities. Results Twenty-six bacterial families with high relative abundance were identified, of which the most common were Staphylococcaceae, Francisellaceae, Corynebacteriaceae, Enterobacteriaceae, Moraxellaceae, Bacillaceae, Halomonadaceae, Xanthomonadaceae, Pseudomonadaceae, Enterococcaceae, Actinomycetaceae and Streptococcaceae. The diversity of the microbial communities in terms of richness and evenness was different at the three study locations, with the PCA showing clear clusters separating the microbial communities in ticks collected at Abu Dhabi, Dubai, and Sharjah. The presence of bacterial families harboring pathogenic genera showed that H. anatolicum could pose a potential threat to livestock and food security in the UAE. Conclusions The study is the first to document important data on the microbial communities associated with H. anatolicum in the UAE. This knowledge will facilitate a better understanding of the distribution pattern of microbes in livestock ticks in the UAE and, ultimately, will aid in deciphering the relationships between microbes and in the exploration of potential factors towards developing effective management strategies. Graphical Abstract

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Description of microbial diversity associated with ticks Hyalomma dromedarii (Acari: Ixodidae) isolated from camels in Hail region (Saudi Arabia) using massive sequencing of 16S rDNA

Cited by 7 publications

References 39 publications

Prevalence, Distribution, and Molecular Record of Four Hard Ticks from Livestock in the United Arab Emirates

Prevalence, Distribution, and Molecular Record of Four Hard Ticks from Livestock in the United Arab Emirates

Assessing Temporal Changes in Microbial Communities in Hyalomma dromedarii Collected From Camels in the UAE Using High-Throughput Sequencing

Microbial composition in Hyalomma anatolicum collected from livestock in the United Arab Emirates using next-generation sequencing

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