Description of “Desulfotomaculum nigrificans subsp. salinus” as a New Species, Desulfotomaculum salinum sp. nov.

Назина, Т. Н.; Rozanova, E. P.; Belyakova, E. V.; Lysenko, Anatoly M.; Полтараус, А. Б.; Tourova, T. P.; Осипов, Г. А.; Belyaev, S. S.

doi:10.1007/s11021-005-0104-x

Cited by 29 publications

(46 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, the dominant OTUs in the clone library were sulfate-reducing bacteria related to T. yellowstonii and D. salinum (SMD-B01 and SMD-B02). It is known that these sulfate reducers are able to grow by fermentation in low-sulfate environments (Henry et al, 1994;Nazina et al, 2005). In addition, a few members of the genus Desulfotomaculum degrade organic matter to H 2 and CO 2 through fermentation process and grow in syntrophic association with hydrogenotrophic methanogens in the absence of sulfate (Imachi et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

“…The SMD-B01 clones accounted for 30% of the clones in the library. SMD-B02 showed the highest identity to the 16S rRNA gene from the cultured organism Desulfotomaculum salinum (85.8% identity), which is able to grow by sulfate reduction or fermentation (Nazina et al, 2005), and accounted for 14% of all the clones in the library. SMD-B03 showed the closest match to the 16S rRNA gene from the cultured organism Burkholderia fungorum (99.6% identity), which is known as an aerobic bacterium (Coenye et al, 2001).…”

Section: Microbial Cell Densitymentioning

confidence: 99%

See 1 more Smart Citation

Microbial methane production in deep aquifer associated with the accretionary prism in Southwest Japan

et al. 2009

View full text Add to dashboard Cite

To identify the methanogenic pathways present in a deep aquifer associated with an accretionary prism in Southwest Japan, a series of geochemical and microbiological studies of natural gas and groundwater derived from a deep aquifer were performed. Stable carbon isotopic analysis of methane in the natural gas and dissolved inorganic carbon (mainly bicarbonate) in groundwater suggested that the methane was derived from both thermogenic and biogenic processes. Archaeal 16S rRNA gene analysis revealed the dominance of H 2 -using methanogens in the groundwater. Furthermore, the high potential of methane production by H 2 -using methanogens was shown in enrichments using groundwater amended with H 2 and CO 2 . Bacterial 16S rRNA gene analysis showed that fermentative bacteria inhabited the deep aquifer. Anaerobic incubations using groundwater amended with organic substrates and bromoethanesulfonate (a methanogen inhibitor) suggested a high potential of H 2 and CO 2 generation by fermentative bacteria. To confirm whether or not methane is produced by a syntrophic consortium of H 2 -producing fermentative bacteria and H 2 -using methanogens, anaerobic incubations using the groundwater amended with organic substrates were performed. Consequently, H 2 accumulation and rapid methane production were observed in these enrichments incubated at 55 and 65 1C. Thus, our results suggested that past and ongoing syntrophic biodegradation of organic compounds by H 2 -producing fermentative bacteria and H 2 -using methanogens, as well as a thermogenic reaction, contributes to the significant methane reserves in the deep aquifer associated with the accretionary prism in Southwest Japan.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Microbial Cell Densitymentioning

confidence: 99%

Microbial methane production in deep aquifer associated with the accretionary prism in Southwest Japan

et al. 2009

View full text Add to dashboard Cite

show abstract

“…Additionally, the reclassification of 'Desulfotomaculum nigrificans subsp. salinus' as a novel species ('Desulfotomaculum salinum') has been proposed (Nazina et al, 2005). Currently, Desulfotomaculum arcticum is the only sulfate-reducing member of the genus that has an optimal temperature above 40 uC and below 50 uC (Vandieken et al, 2006).…”

mentioning

confidence: 99%

Desulfotomaculum alcoholivorax sp. nov., a moderately thermophilic, spore-forming, sulfate-reducer isolated from a fluidized-bed reactor treating acidic metal- and sulfate-containing wastewater

Kaksonen

Spring

Schümann

et al. 2008

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

View full text Add to dashboard Cite

Desulfotomaculum alcoholivorax sp. nov., a moderately thermophilic, spore-forming, sulfate-reducer isolated from a fluidized-bed reactor treating acidic metal-and sulfate-containing wastewater A moderately thermophilic, Gram-positive, endospore-forming, sulfate-reducing bacterium was isolated from a fluidized-bed reactor treating acidic water containing metal and sulfate. The strain, designated RE35E1 T , was rod-shaped and motile. The temperature range for growth was 33-51 6C (optimum 44-46 6C) and the pH range was 6.0-7.5 (optimum pH 6.4-7.3). The strain grew optimally without additional NaCl. The electron acceptors were 10 mM sulfate, thiosulfate and elemental sulfur and 1 mM (but not 10 mM) sulfite. Various alcohols and carboxylic acids were utilized as electron donors. Fermentative growth occurred on pyruvate. The cell wall contained meso-diaminopimelic acid, and the major respiratory isoprenoid quinone was menaquinone MK-7. The major whole-cell fatty acids were iso-C 15 : 0 , iso-C 17 : 1 v10c and iso-C 17 : 0 . Strain RE35E1 T was related to representatives of the genera Desulfotomaculum and Sporotomaculum, the closest relatives being Desulfotomaculum arcticum DSM 17038 T (96.3 % 16S rRNA gene sequence similarity) and Sporotomaculum hydroxybenzoicum DSM 5475 T (92.0 % similarity). Strain RE35E1 T represents a novel species, for which the name Desulfotomaculum alcoholivorax sp. nov. is proposed. The type strain is RE35E1 T (5DSM 16058 T 5JCM 14019 T ).The genus Desulfotomaculum constitutes a group of rodshaped, endospore-forming, sulfate-reducing bacteria. The genus currently includes mesophilic, moderately thermophilic and thermophilic species (Werkman & Weaver, 1927;Campbell & Postgate, 1965;Vandieken et al., 2006). et al., 1996), D. thermosapovorans (Fardeau et al., 1995) and D. thermosubterraneum (Kaksonen et al., 2006b). Additionally, the reclassification of 'Desulfotomaculum nigrificans subsp. salinus' as a novel species ('Desulfotomaculum salinum') has been proposed (Nazina et al., 2005). Currently, Desulfotomaculum arcticum is the only sulfate-reducing member of the genus that has an optimal temperature above 40 uC and below 50 uC (Vandieken et al., 2006). Recently, we reported the isolation of sulfate-reducers from fluidized-bed reactors (FBRs) treating acidic metal-and sulfate-containing wastewater (Kaksonen et al., 2004c). This paper describes one of those isolates, strain RE35E1 T , a novel, moderately thermophilic, spore-forming, sulfatereducing bacterium.Abbreviation: FBR, fluidized-bed reactor.The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain RE35E1 T is AY548778.

show abstract

“…thermobenzoicum (Tasaki et al, 1991); 6, D. thermoacetoxidans (Min & Zinder, 1990); 7, D. geothermicum (Daumas et al, 1988); 8, D. thermocisternum (Nilsen et al, 1996); 9, D. australicum (Love et al, 1993); 10, 'D. salinum' (Nazina et al, 2005). All taxa shared the following characteristics: rod-shaped cells, spore formation, sulfate (10 mM) reduction, utilization of H 2 /CO 2 (80 : 20, v/v) as sole electron and carbon source, and oxidation of lactate (20 mM).…”

mentioning

confidence: 99%

Desulfotomaculum thermosubterraneum sp. nov., a thermophilic sulfate-reducer isolated from an underground mine located in a geothermally active area

Kaksonen

Spring

Schümann

et al. 2006

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

A thermophilic, Gram-positive, endospore-forming, sulfate-reducing bacterium was isolated from an underground mine in a geothermally active area in Japan. Cells of this strain, designated RL50JIIIT, were rod-shaped and motile. The temperature range for growth was 50–72 °C (optimum growth at 61–66 °C) and the pH range was 6.4–7.8 (optimum at pH 7.2–7.4). Strain RL50JIIIT tolerated up to 1.5 % NaCl, but optimum growth occurred in the presence of 0–1 % NaCl. Electron acceptors utilized were sulfate, sulfite, thiosulfate and elemental sulfur. Electron donors utilized were H2 in the presence of CO2, alanine, various carboxylic acids and alcohols. Fermentative growth occurred on lactate and pyruvate. The cell wall contained mesodiaminopimelic acid and the major respiratory isoprenoid quinone was menaquinone 7 (MK-7). Major whole-cell fatty acids were iso-C15 : 0, iso-C17 : 0 DMA (dimethyl acetal), iso-C15 : 0 DMA and iso-C17 : 0. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed 98.7 % similarity with Desulfotomaculum solfataricum DSM 14956T. However, DNA–DNA hybridization experiments with Desulfotomaculum kuznetsovii, Desulfotomaculum luciae and D. solfataricum and the G+C content of the DNA (54.4 mol%) allowed the differentiation of strain RL50JIIIT from the recognized species of the genus Desulfotomaculum. Strain RL50JIIIT therefore represents a novel species, for which the name Desulfotomaculum thermosubterraneum sp. nov. is proposed. The type strain is RL50JIIIT (=DSM 16057T=JCM 13837T).

show abstract

Description of “Desulfotomaculum nigrificans subsp. salinus” as a New Species, Desulfotomaculum salinum sp. nov.

Cited by 29 publications

References 14 publications

Microbial methane production in deep aquifer associated with the accretionary prism in Southwest Japan

Microbial methane production in deep aquifer associated with the accretionary prism in Southwest Japan

Desulfotomaculum alcoholivorax sp. nov., a moderately thermophilic, spore-forming, sulfate-reducer isolated from a fluidized-bed reactor treating acidic metal- and sulfate-containing wastewater

Desulfotomaculum thermosubterraneum sp. nov., a thermophilic sulfate-reducer isolated from an underground mine located in a geothermally active area

Contact Info

Product

Resources

About