Description and sources of contamination by Campylobacter spp. of river water destined for human consumption in Brittany, France

Denis, Martine; Tanguy, Maël; Chidaine, B.; Laisney, Marie-José; Mégraud, Françis; Fravalo, Philippe

doi:10.1016/j.patbio.2009.10.007

Cited by 39 publications

(33 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Campylobacter jejuni and Campylobacter coli are the main species implicated in human Campylobacter infections. These bacteria have been shown to be widespread in the natural aquatic environment (6,11,12,25). Feces of contaminated poultry, cattle, or birds and discharge of sewage constitute sources of pollution for the natural aquatic environment (21,29).…”

mentioning

confidence: 99%

Development of a Rapid and Sensitive Method Combining a Cellulose Ester Microfilter and a Real-Time Quantitative PCR Assay To Detect Campylobacter jejuni and Campylobacter coli in 20 Liters of Drinking Water or Low-Turbidity Waters

Tissier¹,

Denis

Hartemann³

et al. 2012

Appl Environ Microbiol

View full text Add to dashboard Cite

Investigations of Campylobacter jejuni and Campylobacter coli in samples of drinking water suspected of being at the origin of an outbreak very often lead to negative results. One of the reasons for this failure is the small volume of water typically used for detecting these pathogens (10 to 1,000 ml). The efficiencies of three microfilters and different elution procedures were determined using real-time quantitative PCR to propose a procedure allowing detection of Campylobacter in 20 liters of drinking water or low-turbidity water samples. The results showed that more than 80% of the bacteria inoculated in 1 liter of drinking water were retained on each microfilter. An elution with a solution containing 3% beef extract, 0.05 M glycine at pH 9, combined with direct extraction of the bacterial genomes retained on the cellulose ester microfilter, allowed recovery of 87.3% (؎22% [standard deviation]) of Campylobacter per 1 liter of tap water. Recoveries obtained from 20-liter volumes of tap water spiked with a C. coli strain were 69.5% (؎10.3%) and 78.5% (؎15.1%) for 91 CFU and 36 CFU, respectively. Finally, tests performed on eight samples of 20 liters of groundwater collected from an alluvial well used for the production of drinking water revealed the presence of C. jejuni and C. coli genomes, whereas no bacteria were detected with the normative culture method in volumes ranging from 10 to 1,000 ml. In the absence of available epidemiological data and information on bacterial viability, these last results indicate only that the water resource is not protected from contamination by Campylobacter.

show abstract

mentioning

confidence: 99%

Development of a Rapid and Sensitive Method Combining a Cellulose Ester Microfilter and a Real-Time Quantitative PCR Assay To Detect Campylobacter jejuni and Campylobacter coli in 20 Liters of Drinking Water or Low-Turbidity Waters

Tissier¹,

Denis

Hartemann³

et al. 2012

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…in five different rivers in the neighborhoods of Saint-Brieuc, Brittany was investigated over a 12 months period and was found that 50% of the samples were positive for Campylobacter species. C. jejuni was the predominant species in all five rivers, and its sources were linked to poultry (runoff from poultry farms) and human origins (sewage discharge) based on PFGE profiles, whereas C. coli was sharing profiles with isolates from pigs (runoff from swine farms) and C. lari were related to wild birds [39]. In amenity village ponds at South Dalton, Little Weighton and Brantingham in the UK, Campylobacter spp.…”

Section: Surface Watermentioning

confidence: 98%

Campylobacter in the environment: A major threat to public health

Abulreesh¹,

Organji²,

Elbanna³

et al. 2017

APJTD

View full text Add to dashboard Cite

“…counts also naturally vary, due to processes that mobilize them off surfaces and their dilution within surface waters (Sterk et al, 2016). Noting the bias of culture methods to detect C. jejuni and C. coli, C. jejuni has been the species identified most frequently from surface waters (Thomas et al, 1999a), yet C. coli and C. lari are also commonly reported (Thomas et al, 1999a;Denis et al, 2011Hokajärvi et al, 2013, associate with the presence of agricultural runoff or large flocks of waterfowl (Bolton et al, 1987;Obiri-Danso and Jones, 1999). In a study in Ontario, Canada, 73% of isolates from the samples of waterfowl feces (n=15 out of a total of 52 samples) were identified as C. jejuni, 13% were C. coli and 27% C. lari (Van Dyke et al, 2010).…”

Section: Surface Watersmentioning

confidence: 99%

Members of the family Campylobacteraceae: Campylobacter jejuni, Campylobacter coli

Pitkänen¹,

Hänninen²

2019

Water and Sanitation for the 21st Century: Health and Microbiological Aspects of Excreta and Wastewater Management (Global Wate

View full text Add to dashboard Cite

Description and sources of contamination by Campylobacter spp. of river water destined for human consumption in Brittany, France

Cited by 39 publications

References 51 publications

Development of a Rapid and Sensitive Method Combining a Cellulose Ester Microfilter and a Real-Time Quantitative PCR Assay To Detect Campylobacter jejuni and Campylobacter coli in 20 Liters of Drinking Water or Low-Turbidity Waters

Development of a Rapid and Sensitive Method Combining a Cellulose Ester Microfilter and a Real-Time Quantitative PCR Assay To Detect Campylobacter jejuni and Campylobacter coli in 20 Liters of Drinking Water or Low-Turbidity Waters

Campylobacter in the environment: A major threat to public health

Members of the family Campylobacteraceae: Campylobacter jejuni, Campylobacter coli

Contact Info

Product

Resources

About