Desarrollo embrionario y larval del bolo Diplectrum radiale Quoy y Gaimard, 1824 (Pisces: Serranidae)

Abstract

Self-fecundation of Diplectrum radiale a synchronic hermaphrodite species, was induced through a manual process; the fertilization percentage was 86.92 ± 2.75%, producing spherical, floating eggs, with a centric oil drop, a reduced perivitelline space and a smooth envelop. Fertilized eggs had an average diameter of 0.67 ± 0.02 mm and a oil drop with an average diameter of 0.13 ± 0.01 mm at 26.41 ± 0.25°C, pH 8.84 ± 0.17 and 38 ± 1 ppm of salinity. The embryonic development was described, observing the first cleavage at 0 h: 36 min after fecundation at 27.25 ± 0.50°C average temperature. The embryonic development described was similar to other species with pelagic eggs. Hatching started 17 h: 23 min after fertilization at 27°C. At 17 h: 50 min the larval total length was 1.33 ± 0.02 mm, the vitelline sac total length was 0.79 ± 0.03 mm and the oil drop diameter was 0.14 ± 0.01 mm. Growth was constant, decreasing at 43 h when the vitelline sac was almost reabsorbed, restarting growing at 53 h when mouth opened. The last embryonic phases were critical, with a percentage of non viable eggs of 18.89 ± 1.92% at 25.80 ± 0.27°C, pH 6.78 ± 0.08 and 38 ± 1 ppm salinity. At temperatures higher than 28°C embryonic development anomalies were evident with a high percentage of non viable eggs. For this reason it was not possible to compare development between natural and controlled ambient. Embryonic and larval development of D. radiale using the manual induction method permitted to obtain larvae from just one mature individual meaning a reduction in cost and time in producing fish larvae.

Embryonic development of Peruvian grunt <i>Anisotremus scapularis</i> (Perciformes: Haemulidae)

“…The pharyngula stage included the formation of structures such us optical capsules, Kupffer vesicle and somites, and the appearance of melanophores for pigmentation. All of these structures were observed for Peruvian grunt and matched with those described for D. radiale (López et al 2002) The incubation time for of Peruvian grunt was 31-40 h at specific temperature of 19°C, this time is specie-specific and several authors documented that the temperature is one of the main environmental factors that influence the time and percentage of hatching in marine fish (Bobe & Labbé 2010). In some species, this influence was observed for example spotted rose snapper L. guttatus were 15 h at 26.3-28.2 ºC (Boza-Abarca et al 2008) and 21 h at 25-26 °C (Alvarez-Lajonchère et al 2012), for Pacific red snapper L. peru were 23, 20 and 18 h at 26, 28 and 30 °C, respectively.…”

“…However, it was smaller than those reported for black grunt Haemulon bonariense 0.20 ± 0.02 mm (Cuartas et al 2003) and red porgy Pagrus pagrus 0.18-0.20 mm (Machinandiarena et al 2003). In relation to Peruvian grunt newly hatched larvae (2.558 ± 0.051 mm) there were larger than those reported for pond perch Diplectrum radiale 1.33 ± 0.02 mm (López et al 2002), H. bonariense 1.42 + 0.05 mm (Cuartas et al 2003) and colorado snapper Lutjanus colorado 1.9 mm of length (Abdo-de la Parra et al 2014). There are some studies about the size of hatched larvae in which it had been observed a positive correlation between larval size length and egg size, for example Acipense baeri and Acanthopagrus schlegeli (Gisbert et al 2000, Kang et al 2014.…”

Artificial Propagation of Pomadasys hasta (Bloch, 1790): A Key to Reach Sustainable Aquaculture

“…The egg diameter of European sea bass Dicentrarchus labrax were 1.162±0.004 mm (Saka et al, 2001) and gilthead sea bream Sparus aurata were 1.001±0.005 mm (Kamacı et al, 2005) which is similar with the present study (1.1-1.3 mm). In relation to P. hasta newly hatched larvae (1.6-1.8 mm) there were slightly larger than those reported for pond perch Diplectrum radiale 1.33±0.02 mm (López et al, 2002), H. bonariense 1.42 + 0.05 mm (Cuartas et al, 2003) and somewhat smaller than colorado snapper Lutjanus colorado 1.9 mm of length (Abdode la Parra et al, 2014). Newly hatched larvae (1.5-1.7 mm) of S. argus fish in the same order was concomitant with the present study (Su et al, 2019).…”

Desarrollo embrionario-larval y tiempo de metamorfosis del pez tropical <i>Xenomelaniris brasiliensis</i> (Pisces: Atherinidae)

“…Sprague et al (2001), López et al (2002) afirman que las divisiones consecutivas de los blastómeros no están separadas por períodos de crecimiento y los blastómeros resultantes tienen sólo la mitad del tamaño original después de cada división. Durante el proceso de desarrollo se observó la vesícula de Kupffer.…”

“…López et al (2002) refieren un tiempo de eclosión de 17: 23 horas a 27ºC para Diplectrum radiale, Cuartas et al (2002) afirma que el tiempo de eclosión para Haemulom bonariense fue de 15:19 horas a 27ºC. Todas las especies mencionadas anteriormente al igual que la especie estudiada, son tropicales y en ninguno de estos casos los huevos sobrepasaron los 0.8 mm de diámetro.…”