Dermal carbonyl modification is related to the yellowish color change of photo-aged Japanese facial skin

Ogura, Yuki; Kuwahara, Tomohiro; Akiyama, Minoru; Tajima, S.; Hattori, Kazuhisa; Okamoto, Koji; Okawa, Shinpei; Yamada, Yukio; Tagami, Hachiro; Takahashi, Masanori; Hirao, Tetsuji

doi:10.1016/j.jdermsci.2011.06.015

Cited by 44 publications

(45 citation statements)

References 36 publications

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“…A,B). Faint but definite yellowish color observed in repetitive UVA‐irradiated fibroblasts was consistent with the findings previously reported by others which showed accumulation of two classes of oxidized proteins, carbonyl protein and advanced glycation end products in photoaged dermis .…”

Section: Resultssupporting

confidence: 92%

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Establishment of Photoaging In Vitro by Repetitive UVA Irradiation: Induction of Characteristic Markers of Senescence and its Prevention by PAPLAL with Potent Catalase Activity

Yoshimoto

Yoshida

Ando

et al. 2018

Photochem & Photobiology

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To understand a role of UVA radiation in photoaging of the skin, we established a model of photoaging cells using cultured human dermal fibroblasts. Repeated low-dose UVA radiation for 10 consecutive days induced senescence in fibroblasts, characterized with (1) increased level of senescence-associated β-galactosidase, (2) flattened large cell shape, (3) accumulation of reactive oxygen species, (4) yellowish coloration and (5) expression of p16. These were also observed in chronologically aged fibroblasts (doubling times >20), whereas none of these were detected in young cells (doubling times <10). Collectively, we propose that fibroblasts exposed to repetitive UVA radiation may be a good model of aged cells to study the mechanism of aging and photoaging and further to search for novel agents preventing cellular senescence. In addition, H O was produced in the culture medium by a single low dose of UVA irradiation. Further, PAPLAL, a nanoparticle of platinum and palladium having potent catalase-like activity, significantly delayed the onset of H O -induced cell senescence. The present study strongly indicates that repetitive short-term UVA irradiation induces aging of cells possibly via H O and may be suppressed by potent anti-H O agents.

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Section: Resultssupporting

confidence: 92%

“…Yellowish color change has been proposed to be caused by accumulation of oxidized proteins, carbonylated proteins and advanced glycation end products (AGEs) in dermis, in addition to the increase of melanin in epidermis . Yellow color products by the oxidation of proteins may be triggered by high level of H 2 O 2 yielded by UVA radiation.…”

Section: Discussionmentioning

confidence: 99%

Establishment of Photoaging In Vitro by Repetitive UVA Irradiation: Induction of Characteristic Markers of Senescence and its Prevention by PAPLAL with Potent Catalase Activity

Yoshimoto

Yoshida

Ando

et al. 2018

Photochem & Photobiology

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“…One of the most distinguishable characteristics of glycated skin is its color change to yellow. 20) As shown in Figs. 2a and b, glycated skin showed a yellowish tinge.…”

Section: Resultsmentioning

confidence: 79%

“…It is well known that increases in the b* value and autofluorescence reflect the accumulation of AGEs in skin. 19,20) Glycation induced by 50 mM GO showed 1.6 times higher autofluorescence and 2.1 times higher b* values than normal skin (Figs. 2a, b).…”

Section: Discussionmentioning

confidence: 94%

Permeation of Hydrophilic Molecules across Glycated Skin Is Differentially Regulated by the Stratum Corneum and Epidermis–Dermis

Yokota

Tokudome

2015

Biological & Pharmaceutical Bulletin

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The effects of glycation on skin permeation and accumulation of compounds were evaluated using an in vitro glycated skin model. Glycation of the skin of hairless mice was induced using vertical diffusion cells and incubation with phosphate-buffered saline containing 50 mM glyoxal for 24 h. Flux and accumulation in the skin were determined by applying hydrophilic and lipophilic molecules (Sodium fluorescein; FL-Na and Nile red, respectively) to this in vitro glycated skin model. Furthermore, to investigate the effect of glycation on epidermal-dermal barrier properties, we conducted diffusion experiments with FL-Na and fluorescein isothiocyanate-dextran using stratum corneum (SC)-stripped glycated skin. The in vitro glycated skin model demonstrated characteristic glycation alterations like a yellowish change in skin color and surface roughness. For low-molecular weight (MW) hydrophilic molecules, flux across glycated full-thickness skin was higher than that across normal skin, although there was no difference with lipophilic molecules. However, glycated epidermis-dermis showed lower flux, and the difference increased with the MW of the compound. Furthermore, the amount of high-MW hydrophilic molecules accumulated in glycated epidermis-dermis was decreased. These results suggest that glycated SC and epidermis-dermis differentially regulate the permeability of hydrophilic molecules and highlight the importance of controlling drug delivery by modifying the formulation or method of application depending on skin condition. Key words skin permeation; advanced glycation end product; stratum corneum As a route of drug administration, the skin attracts much attention due to non-invasive application, ease of dose control and absorption without hepatic first-pass metabolism. However, human skin changes over time due to chronological aging, exposure to UV radiation, and diseases such as atopic dermatitis and psoriasis. With the increase in the aging population in recent decades, there is a growing need to care for a variety of skin types. Therapeutic applications involving the skin have also increased in diversity in recent years. A variety of chemical and physiological methods are being studied to lower the barrier posed by the skin's outermost layer, the stratum corneum (SC).1) Consisting of corneocytes and intercellular lipids, the SC plays an important role as a rate limiting layer for the penetration of compounds. Serving as the major protection from foreign chemicals and water loss, and also associated with body temperature regulation, the SC is permeable to only lipophilic and <500 Da compounds.2) Therefore, various biochemical approaches to increase permeability across the SC have been attempted over the years.3) Physical methods such as microneedles, thermal ablation and microdermabrasion are also used to enhance the efficiency of penetration by piercing or removing the SC. Additionally, iontophoresis and sonophoresis increase skin permeability by electrical driving force and ultrasound, respectively. 4)Despite many ...

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“…In a future study, we need to improve the condition to induce glycation to cells. We used glyoxal as glycation inducer in the present study, which is widely used in in vitro studies [28,29]. Glyoxal is one of the glycation inducers produced by spontaneous degradation of glucose [30].…”

Section: Discussionmentioning

confidence: 99%

The Effect of Glycation on Epidermal Lipid Content, Its Metabolism and Change in Barrier Function

Yokota¹,

Tokudome²

2016

Skin Pharmacol Physiol

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Background/Aims: Advanced glycation end products, which are linked to both aging and hyperglycemia, cause marked functional and structural alterations in human skin. Though it is well known that the metabolism of glucose is closely associated with that of fatty acid (FA), sharing the same energy-yielding reaction pathways as glucose, its effect on the epidermis has been unclear so far. Methods: Content of ceramides, cholesterol and FA in a reconstructed epidermal model glycated by glyoxal was analyzed by high-performance thin-layer chromatography. FA species extracted from HaCaT keratinocytes was determined by gas chromatography/mass spectrometry. Regulation of FA synthesis was analyzed by real-time PCR. For physiological analysis, excised mouse skin was glycated using a vertical diffusion cell and used for the evaluation of barrier function by transepidermal water loss measurement and observation of penetration of sodium fluorescein. Results: Saturated FA content was significantly increased in glycated epidermis, and glycation upregulated mRNA expression of FA elongases 2 and 3 and FA synthase in HaCaT cells. Further, both inside-out and outside-in barriers were disrupted in glycated excised skin. Conclusion: Biological and physical change in the epidermis, especially upregulation of FA synthesis by glycation, contributed to barrier disruption, and inhibiting glycation may offer an effective treatment option for aged or glycated skin.

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Dermal carbonyl modification is related to the yellowish color change of photo-aged Japanese facial skin

Cited by 44 publications

References 36 publications

Establishment of Photoaging In Vitro by Repetitive UVA Irradiation: Induction of Characteristic Markers of Senescence and its Prevention by PAPLAL with Potent Catalase Activity

Establishment of Photoaging In Vitro by Repetitive UVA Irradiation: Induction of Characteristic Markers of Senescence and its Prevention by PAPLAL with Potent Catalase Activity

Permeation of Hydrophilic Molecules across Glycated Skin Is Differentially Regulated by the Stratum Corneum and Epidermis–Dermis

The Effect of Glycation on Epidermal Lipid Content, Its Metabolism and Change in Barrier Function

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