DERIVING BIOLOGICALLY ACTIVE PEPTIDES AND STUDY Of THEIR QUALITIES

Abstract

The main component of human health is nutrition which becomes even more important if a person is sick. In the body of cancer patients metabolic processes change, so he/she requires a special diet. Probiotics and short peptides are the integral part of the diet of cancer patients. One of the goals of nutritional support for cancer patients is the visceral protein pool maintenance. In modern formulae for enteral support for cancer patients the protein component can be represented in one of three types: native protein; cow’s milk whey/whole protein peptides; free amino acids. The present article deals with study on biotechnological treatment influence on milk protein controlled hydrolysis process with the aim of deriving biologically active peptides, as well as of studying several qualities: immunomodulatory, cytotoxic, antioxidant and prebiotic. Conditions for enzymic hydrolysis of milk proteins are optimised, which provide deriving biologically active directional peptides. The optimal parameters of hydrolysis conduction are chosen, which provide the polypeptide chain division into peptides and free amino acids, as well as enzymic system was chosen, consisting of chymotrypsin or thermolysin at a temperature of 37±2°C, in the enzyme-substrate ratio of 1 to 50 and process time of 12.00±0.05 h. The qualities of biologically active peptides (immunomodulatory, cytotoxic, antioxidant and prebiotic), derived from milk proteins, are studied. The studies demonstrated that all peptides under study have anti-tumour qualities, at the same time increase in biopeptide concentration causes decrease in the capacity for survival of different line cancer cells