Derivatization of hydroxyl functional groups for liquid chromatography and capillary electroseparation

Escrig-Doménech, Aarón; Simó‐Alfonso, Ernesto Francisco; Herrero‐Martínez, José Manuel; Ramis‐Ramos, Guillermo

doi:10.1016/j.chroma.2013.04.027

Cited by 42 publications

(36 citation statements)

References 115 publications

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“…Derivatization for improvement of UV–vis detection by chromophore labeling is carried out for species containing aliphatic or aromatic hydroxyl groups, amino, carboxyl, carbonyl, tio or combination of them (Escrig‐Doménech, Simó‐Alfonso, Herrero‐Martínez, & Ramis‐Ramos, ). Derivatization for fluorescence detection is more sensitive for bioanalysis, and can be done with fluorescent reagents, such as dansyl chloride, or with nonfluorescent reagents, such as o ‐phthalaldehyde that produces fluorescent compounds with analytes containing amino groups (Moldoveanu & David, ).…”

Section: Derivatization In Bioanalysismentioning

confidence: 99%

Sample preparation for large‐scale bioanalytical studies based on liquid chromatographic techniques

Medvedovici

Bacalum

David

2017

Biomedical Chromatography

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Quality of the analytical data obtained for large-scale and long term bioanalytical studies based on liquid chromatography depends on a number of experimental factors including the choice of sample preparation method. This review discusses this tedious part of bioanalytical studies, applied to large-scale samples and using liquid chromatography coupled with different detector types as core analytical technique. The main sample preparation methods included in this paper are protein precipitation, liquid-liquid extraction, solid-phase extraction, derivatization and their versions. They are discussed by analytical performances, fields of applications, advantages and disadvantages. The cited literature covers mainly the analytical achievements during the last decade, although several previous papers became more valuable in time and they are included in this review.

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Section: Derivatization In Bioanalysismentioning

confidence: 99%

Sample preparation for large‐scale bioanalytical studies based on liquid chromatographic techniques

Medvedovici

Bacalum

David

2017

Biomedical Chromatography

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“…[33] used 2-fluoro- N -methylpyridinium salts as derivatizating agents for determination of free alcohols and low ethoxymers. The group of Ramis-Ramos [34] has published interesting applications for the following analytical characterization reagents: maleic anhydride, phthalic anhydride, and diphenic anhydride. Sparham et al .…”

Section: Introductionmentioning

confidence: 99%

Determination of Dodecanol and Short‐Chained Ethoxylated Dodecanols by LC–MS/MS (with Electrospray Ionization) After Their Derivatization (with Phenyl Isocyanate)

Zembrzuska

2017

J Surfact & Detergents

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This report describes the application of LC–MS/MS for the separation of dodecanol (C12OH) and homogenous fatty alcohols ethoxylated (AE) containing a dodecyl moiety and 1–9 ethoxy groups. These ethoxylates and free alcohol were derivatized for LC–MS/MS analysis with phenyl isocyanate (PIC). The derivatives of analytes with PIC were separated using a C18 column. Gradient elution with a mixture of ethyl acetate and acetonitrile (5 mM) was employed. The described determination method is characterized by low detection limits (range from 0.005 µg L−1 for: C12OH, C12EO2–7 to 1 µg L−1 for C12EO1) and quantification limits (range from 0.01 µg L−1 for: C12EO5–7 to 2 µg L−1 for C12EO1). The developed and validated method was used in combination with liquid–liquid extraction (using ethyl acetate) in order to identify and quantitatively determine the C12OH and C12EO1–9 present in environmental samples collected from Warta river water in Poznan.Graphical abstract Electronic supplementary materialThe online version of this article (doi:10.1007/s11743-017-2015-z) contains supplementary material, which is available to authorized users.

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“…Although these reagents are generally used in a dry solvent with a base, they can also be used under alternative conditions. 9,[13][14][15][16][17][18][19] In addition, 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F), which also reacts with amines, phenols, and water, has been used in aqueous solution at neutral and basic pH values, and the derivatization reaction can be terminated by the addition of an acid. 20,21 However, as these derivatization methods are not only applicable to aromatic amines, but also to phenols and aliphatic amines, a sample containing an interfering substance must first be cleaned up or pretreated by, for example, solid-phase extraction or liquid-liquid extraction methods.…”

Section: Introductionmentioning

confidence: 99%

“…[9][10][11][12] For example, acid chloride reagents are extremely reactive derivative reagents, which react with water, alcohols, phenols, amines, and aromatic amines. Although these reagents are generally used in a dry solvent with a base, they can also be used under alternative conditions.…”

Section: Introductionmentioning

confidence: 99%

Selective Detection of Carcinogenic Aromatic Diamines in Aqueous Solutions Using 4-(N-Chloroformylmethyl-N-methylamino)-7-nitro- 2,1,3-benzoxadiazole (NBD-COCl) by HPLC

Inoue

2017

ANAL. SCI.

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Reaction conditions for the selective derivatization of three types of aromatic diamines without clean-up in an aqueous solution using 4-(N-chloroformylmethyl-N-methylamino)-7-nitro-2,1,3-benzoxadiazole (NBD-COCl) have been developed for simple, fast, and selective analysis by HPLC. The aromatic amines were derivatized to give di-NBD-CO-aromatic amines at 35 C for 5 min in a buffer solution at pH 5 containing a low acetonitrile concentration (0 -400 ng/mL, n = 5, r >0.998). The retention times of these di-NBD-CO-aromatic amines were <5 min. Sample solutions containing aromatic amines, phenols, and an aliphatic amine were also prepared. Under the optimized reaction conditions (i.e., low acetonitrile content and acidic conditions), no derivatization of the phenols and the aliphatic amine was observed. These were related to the differences in the pKa values of the target substances, the organic solvent concentration, and the reaction solution pH. For the model system, a sulfuric acid-impregnated filter was spiked with the aromatic amines, phenols, and aliphatic amine prior to extraction, derivatization, and measurement by HPLC. Only aromatic amines were detected quantitatively, with no other compounds being observed.

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Derivatization of hydroxyl functional groups for liquid chromatography and capillary electroseparation

Cited by 42 publications

References 115 publications

Sample preparation for large‐scale bioanalytical studies based on liquid chromatographic techniques

Sample preparation for large‐scale bioanalytical studies based on liquid chromatographic techniques

Determination of Dodecanol and Short‐Chained Ethoxylated Dodecanols by LC–MS/MS (with Electrospray Ionization) After Their Derivatization (with Phenyl Isocyanate)

Selective Detection of Carcinogenic Aromatic Diamines in Aqueous Solutions Using 4-(N-Chloroformylmethyl-N-methylamino)-7-nitro- 2,1,3-benzoxadiazole (NBD-COCl) by HPLC

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