Selective Detection of Carcinogenic Aromatic Diamines in Aqueous Solutions Using 4-(N-Chloroformylmethyl-N-methylamino)-7-nitro- 2,1,3-benzoxadiazole (NBD-COCl) by HPLC

Abstract

Reaction conditions for the selective derivatization of three types of aromatic diamines without clean-up in an aqueous solution using 4-(N-chloroformylmethyl-N-methylamino)-7-nitro-2,1,3-benzoxadiazole (NBD-COCl) have been developed for simple, fast, and selective analysis by HPLC. The aromatic amines were derivatized to give di-NBD-CO-aromatic amines at 35 C for 5 min in a buffer solution at pH 5 containing a low acetonitrile concentration (0 -400 ng/mL, n = 5, r >0.998). The retention times of these di-NBD-CO-aromatic amines were <5 min. Sample solutions containing aromatic amines, phenols, and an aliphatic amine were also prepared. Under the optimized reaction conditions (i.e., low acetonitrile content and acidic conditions), no derivatization of the phenols and the aliphatic amine was observed. These were related to the differences in the pKa values of the target substances, the organic solvent concentration, and the reaction solution pH. For the model system, a sulfuric acid-impregnated filter was spiked with the aromatic amines, phenols, and aliphatic amine prior to extraction, derivatization, and measurement by HPLC. Only aromatic amines were detected quantitatively, with no other compounds being observed.

Porous Particle‐Based Inkjet Printing of Flexible Fluorescent Films: Enhanced Sensing Performance and Advanced Encryption

“…[37,38] Therefore, fluorescence responses of the two films to different amines in vapor phase were tested. [37,38] Therefore, fluorescence responses of the two films to different amines in vapor phase were tested.…”

“…It is reported that NBD derivatives are widely used in amine sensing owing to its sensitivity to the presence of the amines. [37,38] Therefore, fluorescence responses of the two films to different amines in vapor phase were tested. As expected, the fluorescence emission of the two films was quenched when the tested amines were pumped, which should be explained by the photoinduced electron transfer (PET) process between NBDCOOH and diethylamine.…”

The Utility of Sonogashira Coupling Reaction for the Derivatization of Aryl Halides with Fluorescent Alkyne

“…A large number of reagents have been developed for the fluorescence derivatization of various functional groups such as amines, phenols, thiols, aldehydes and carbonyl groups. [1][2][3][4][5][6][7] However, conventional fluorescence derivatization reagents cannot be applied to many important compounds that do not possess such typical functional groups. An important example of such compounds is aryl halides.…”

Simple, selective, and identifiable analysis of aromatic monoamines with a surrogate on sulfuric acid impregnated filters by derivatization with an acid chloride reagent and HPLC with fluorescence detection

“…Derivatization reactions are commonly used to enhance and separate the analytical signals for LC, CE, TLC, and so on [14][15][16][17][18][19][20][21][22]. Acid chlorides, which are highly reactive derivatization reagents, react with samples for a short time and produce the corresponding derivatives in addition to a chloride salt.…”