Deregulation of miR‐92a expression is implicated in hepatocellular carcinoma development

Abstract

MicroRNAs (miRNAs) belong to a class of the endogenously expressed non-coding small RNAs which primarily function as gene regulators. Growing evidence suggests that miRNAs have a significant role in tumor development and may constitute robust biomarkers for cancer diagnosis and prognosis. The miR-17-92 cluster especially is markedly overexpressed in several cancers, and is associated with the cancer development and progression. In this study, we have demonstrated that miR-92a is highly expressed in hepatocellular carcinoma (HCC). In addition, the proliferation of HCC-derived cell lines was enhanced by miR-92a and inhibited by the anti-miR-92a antagomir. On the other hand, we have found that the relative amount of miR-92a in the plasmas from HCC patients is decreased compared with that from the healthy donors. Interestingly, the amount of miR-92a was elevated after surgical treatment. Thus, although the physiological significance of the decrease of miR-92a in plasma is still unknown, deregulation of miR-92 expression in cells and plasma should be implicated in the development of HCC.

HepatomiRNoma: The proposal of a new network of targets for diagnosis, prognosis and therapy in hepatocellular carcinoma

“…The only study conducted by Shigoka et al (2010) showed that it is highly expressed in HCC tissue and is reduced in the plasma of HCC patients. However, after surgical resection, it tends to decrease in the tissue and to increase in the plasma, thus indicating a prognostic role.…”

Diagnostic and prognostic significance of miRNA signatures in tissues and plasma of endometrioid endometrial carcinoma patients

“…As a result decreased expression of miRNAs would be observed in plasma. [45][46][47] Two single miRNAs, miR449a and miR-1228 showed high sensitivity and specificity in classifying EEC and control samples. Moreover, logistic regression analysis revealed two miRNA signatures: miR-9/ miR-1228 and miR-9/miR-92a, which classified EEC plasma samples with higher accuracy in comparison to single miRNAs.…”

Expression profiling of miR-96, miR-584 and miR-422a in colon cancer and their potential involvement in colon cancer pathogenesis

“…The distinction was remarkable and thus the levels of miR-96/miR-638 have the potential as a viable non-invasive biomarker for colon cancer. Similar quantification studies were carried out in the plasma samples of hepatocellular carcinoma patients to identify potential biomarkers [14].…”

“…We measured the expression levels of miR-96, miR-584 and miR-422a independently in plasma samples from 25 colon cancer patients with respect to the healthy individual samples by quantitative RT-PCR. Recent studies have shown that, since the levels of plasma miR-638 are quite stable, miR-638 can be used as a standard to improve the precision of the analysis [14]. Therefore, we analyzed the ratios of miR-96, miR-584 and miR422a levels to miR-638 levels in the plasma samples from healthy and colon cancer patients.…”