Der Einfluß von Umweltbedingungen auf die ArtkreuzungDigitalis purpurea L. ×Digitalis lutea L.

Martin, Stein

doi:10.1007/bf00709192

Cited by 4 publications

(2 citation statements)

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“…Attention has been directed toward the endogenous lipid synthetic processes in blood vessels because of the possible role they may play in the accumulation of lipid substances in the atherosclerotic plaque (6,(8)(9)(10)(11). Although the synthesis of phospholipids has been demonstrated in blood vessels of both experimental animals and humans (2,9,(12)(13)(14)(15)(16)(17), the significance of the phospholipid synthetic mechanism is unknown. Zilversmit and his coworkers (18,19) have suggested that this synthesis is accelerated in experimental atherosclerosis and that this represents an effort on This work was supported by research grants from the Washington State Heart Association (6566-8) and U. S. Public Health Service (5 RO1 AM 08569-02, 2 RO1 AM 08368-09A1, AM 02456-07).…”

Section: Additional Abstractmentioning

confidence: 99%

In Vitro Studies of Phospholipid Synthesis in Experimental Atherosclerosis

Párker

Ormsby

Peterson

et al. 1966

Circulation Research

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Using in vitro techniques and labeled linoleic acid and glucose, alterations in phospholipid synthesis in the aorta were correlated with electron microscopic studies at various intervals of time after feeding rabbits cholesterol. After 4 to 8 weeks of feeding, more phospholipid precursors were incorporated into die phospholipids of atherosclerotic blood vessels than of normal vessels. Concomitant with the metabolic alterations, the following ultrastructural changes occur. Smooth muscle cells of the plaque (myo-intimal cells) evolve into highly vacuolated cells containing a profusion of cytoplasmic organelles. The increase in membranous organelles suggests that the increase in phospholipid synthesis may be the result of a cellular requirement for increased intracytoplasmic structural phospholipid. ADDITIONAL KEY WORDSatherosclerotic plaque smooth muscle cells phospholipid metabolism experimental atherosclerosis glucose metabolism intracellular organelles phospholipid synthesis electron microscopy linoleic acid blood vessel metabolism rabbit aorta• The metabolic capabilities of the arterial wall have come under scrutiny in the past few years in attempts to elucidate the relationship of vessel wall metabolism to atherosclerosis (1)(2)(3)(4)(5)(6)(7)(8). Attention has been directed toward the endogenous lipid synthetic processes in blood vessels because of the possible role they may play in the accumulation of lipid substances in the atherosclerotic plaque (6,(8)(9)(10)(11). Although the synthesis of phospholipids has been demonstrated in blood vessels of both experimental animals and humans (2,9,(12)(13)(14)(15)(16)(17), the significance of the phospholipid synthetic mechanism is unknown. Zilversmit and his coworkers (18,19) have suggested that this synthesis is accelerated in experimental atherosclerosis and that this represents an effort on This work was supported by research grants from the Washington State Heart Association (6566-8) and U. S. Public Health Service (5 RO1 AM 08569-02, 2 RO1 AM 08368-09A1, AM 02456-07).Accepted for publication June 22, 1966. the part of the vascular wall to utilize the surface active properties of phospholipids to solubilize cholesterol accumulating in the plaque. The capacity of the vascular wall to synthesize phospholipids is apparently related to its ability to metabolize glucose, because recent investigations have suggested that the products of glycolysis and oxidation phosphorylation (a-glycerophosphate and ATP) are requirements for phosphatdde synthesis (15, 17).It has been proposed on morphological grounds that the cells responsible for this increased phospholipid metabolism are those accumulating in the intimal plaques (24). These cells have been variously labeled fibroblasts, monocytoid cells and macrophages, but recent electron microscopic studies have clearly identified one predominant cell type to be smooth muscle (24,25). In one of these studies (24), special attention was directed to the smooth muscle cells in the plaque (called myo-intimal cells) and the...

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Section: Additional Abstractmentioning

confidence: 99%

In Vitro Studies of Phospholipid Synthesis in Experimental Atherosclerosis

Párker

Ormsby

Peterson

et al. 1966

Circulation Research

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“…Haploid Digitalis purpurea seeds were obtained in rare cases as the result of apomixis. From these seeds dwarfish plants were grown (2). Fertilisation of D. lanata flowers with X-rays treated pollen (1.29 and 7.7 (Ckg ±1 ) resulted in the formation of chimerical plants containing cells which obviously were haploid (3).…”

Section: Introductionmentioning

confidence: 99%

Haploid Plants Regenerated from Androgenic Cell Cultures of Digitalis lanata

Diettrich¹,

Ernst²,

Luckner³

2000

Planta Med

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Androgenic callus was obtained from cold treated anthers and pollen of Digitalis lanata. The callus was mixoploid and contained haploid, diploid and tetraploid cells as shown by impulse cytophotometry. Haploid cell lines were selected by colony cloning. They were unstable and selection had to be repeated every 1-2 months. Mixoploid shoot cultures were derived from embryogenic haploid cell lines via somatic embryos. Haploid shoots were selected by explanting shoot tips. The shoots showed wide variability in cardenolide content and profile. Rooting of the haploid shoots resulted in haploid plants. These plants were smaller in size than diploid plants. Often the flowers were morphologically abnormal and showed male sterility due to crippled anthers.

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