Der Einfluß von Phosphat-Mangel-Ernährung auf die Synthese der Phospho- und Glykolipide bei Impatiens

Tevini, Manfred

doi:10.1016/s0044-328x(71)80009-0

Cited by 10 publications

(2 citation statements)

References 22 publications

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“…Lipid analyses of the tissues of higher plants invariably report substantial amounts of PE (1,5,6,16,21,27,30,34,36). On the other hand, PS is less frequently reported (5,6,16,34).…”

Section: Discussionmentioning

confidence: 99%

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Biosynthesis of Phosphatidylethanolamine by Enzyme Preparations from Plant Tissues

Macher

Mudd²

1974

Plant Physiol.

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(7) (3) However, reaction 7 is clearly an exchange reaction and can only lead to net synthesis of PE if the organism can make PS (e.g. by reaction 2) and ethanolamine.The cells of higher plants have both procaryotic and eucaryotic characteristics exemplified by the nucleic acids of the nuclei and other subcellular organelles. It is, therefore, of some interest to examine the biosynthesis of PE in higher plants to see whether the mechanism of biosynthesis is by the prevalent method in animals, the method in bacteria, or both. The reaction studied in this paper is the formation of PE from CDPethanolamine (reaction 6). PS -> PE + CO2Our understanding of the biosynthesis of phospholipids in animal tissues stems from the basic findings of 9,12,19,20 MATERIALS AND METHODSMaterials. Plant materials were purchased from local grocery stores. CDP-ethanolamine was prepared by the method of Kennedy (19) or purchased from Sigma Chemical Company, St. Louis, Missouri, CDP-choline was purchased from Sigma Chemical Company. "4C-CDP-choline was purchased from International Chemical and Nuclear Corporation, Irvine, California. "4C-Phosphoryl ethanolamine was obtained from New England Nuclear."4C-CDP-ethanolamine was prepared from "4C-phosphoryl ethanolamine using enzyme preparations from chicken liver as described by Chojnacki and Metcalf (10). The product was isolated by ion exchange chromatography on Dowex-1-formate according to the method of Kennedy (19). Purity of the product was evaluated by paper chromatography in solvent systems recommended by Scheider (31) and scanning of the paper strip with a radioactivity scanner.Methods. Plant extracts were prepared by homogenizing a given weight of material with twice as much homogenizing medium. The homogenizing medium consisted of 0.25 M sucrose, 10 mM tris-HCl pH 7.5, 1 mm EDTA. The plant material and cold medium were homogenized in a Waring Blendor (three 5-sec bursts), and the homogenate was pressed through two layers of cheesecloth. In the preparation of subcellular fractions, the filtrate was first centrifuged for 5 min at 1,000g. The supernatant was centrifuged for 30 min at 20,000g and centrifuged for 60 min at 100,000g. The first two pellets (1,OOOg and 20,000g) were suspended in 20 ml each 10 mm tris-HCI pH 7.5, 1 mM EDTA, and the l00,OOOg pellet in 10 ml of the same medium. Reaction mixtures contained 2 mm DTT, 20 mM

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“…Lipid analyses of the tissues of higher plants invariably report substantial amounts of PE (1,5,6,16,21,27,30,34,36). On the other hand, PS is less frequently reported (5,6,16,34).…”

Section: Discussionmentioning

confidence: 99%

“…On the other hand, PS is less frequently reported (5,6,16,34). If PS is the precursor of PE, as it is in bacteria (28), only small amounts may accumulate before decarboxylation takes places.…”

Section: Discussionmentioning

confidence: 99%