Der Einfluß verschiedener Pharmaka auf die Verteilung eines markierten Endotoxins im Organismus

Fritze, E.; Doering, P; Herlyn, Holger

doi:10.1007/bf02049450

Cited by 3 publications

(2 citation statements)

References 18 publications

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“…The choice of a radioactive marker is of crucial importance for in viva studies on uptake and subsequent distribution of endotoxin, l~dioactive labels such as CrS~Cl8 and ps~ were not employed here but the results of others (7,9,11,12,14) have shown that these labels, presumably with endotoxin attached, are removed more rapidly from plasma than is endotoxin tagged with NasCrSO4 (reference 3 together with the present report). No evidence is available to show that the Cr51C13 label is fixed onto the endotoxin either before or after its administration.…”

Section: Discussionmentioning

confidence: 92%

“…No evidence is available to show that the Cr51C13 label is fixed onto the endotoxin either before or after its administration. That the difference between the in dvo distribution of endotoxinbound CrSlC18 and free CrS~C18 indicates that the label remains associated with the endotoxin in the former case, is not necessarily a valid assumption (3,7,9). For example, if in dvo liberated chromium is not free but instead attached to a small dialyzable molecule (10), its subsequent distribution may be changed appreciably from that of the free label alone.…”

Section: Discussionmentioning

confidence: 98%

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CHARACTERIZATION OF A CR51-LABELED ENDOTOXIN AND ITS IDENTIFICATION IN PLASMA AND URINE AFTER PARENTERAL ADMINISTRATION

Chedid

Skarnes

Parant

1963

The Journal of Experimental Medicine

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Radioisotopes such as 1 ~, Na2C#tO,, and Cr61C18 have been utilised for marking endotoxin in efforts to determine in ~ivo uptake and distribution of the latter material (3, 7-9, 11, 12, 14). These reports are in fair agreement conceming the quantitative distribution of the endotoxin as based on subsequent cotmting of plasma and organ samples. Until recently, evidence for the presence of endotoxin itself in tissues and fluids has been presumed on the basis of the following two observations; (a) differences in in ~/ro distribution of the free label as contrasted to that of the label bound to endotoxin and (b) the increased rate of plasma clearance of radioactivity in animals rendered tolerant to endotoxins. Current studies have shown that a correlation exists between the toxicity and the radioactivity present in plasma samples collected at various times after administration of a chromate-labded endotoxin (6). Furthermore, the presence of endotoxin in such plasma samples was demonstrated immunologically by means of ring test precipitation (5). However, evidence has been lacking that the endotoxin moiety per se bears the radioactive label either before injection or after recovery from the tissues and fluids of recipient animals. It is the purpose of this report to show that the labeling of endotoxin with hexavalent Cr 51 results in a specifically tagged product, a part of which retains both label and toxicity in circulating plasma for several hours after administration. Furthermore, it will be shown that endotoxin is demonstrable in urine but in a non-toxic and non-labeled state. Materials and MethodsEndo~o~n.--The source of endotoxin was the Danysz strain of Salmonella entedtldls prepared by the method of Boivin (1). The product was labeled by incubating 100 mg with 1.5 me of Na¢C#IO4 (specific activity --73 mc/mg), during 48 hours according to a method of Brande, e~ al. (2). After labeling, the specific activity of the endotoxin was 1.5 #c/rag which

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