Deproteinizing Agents as an Effective Enamel Bond Enhancer-An in Vitro Study

Hasija, Pallavi; Sachdev, Vinod; Mathur, Shivani; Rath, Rishi

doi:10.17796/1053-4628-41.4.280

Cited by 14 publications

(14 citation statements)

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“…e mean SBS of the bromelain-treated group after application of 37% acid phosphoric was not significantly different from that of the group only etched with 37% phosphoric acid (G5), which is in agreement with a previous study [11]. e similarity of the results may be due to the use of bromelain after the acid etching process.…”

Section: Discussionsupporting

confidence: 90%

“…In line with our results, Hasija et al compared the effect of papain gel on the SBS of composite resin to primary teeth enamel and found no statistically significant difference between the groups [11]. In contrast to the results of the present study, some previous studies indicated that enamel deproteinization with papain gel increased the shear bond strength, irrespective of the acid phosphoric etching application [5,8,10].…”

Section: Discussionsupporting

confidence: 85%

“…Bromelain is yet another proteolytic enzyme derived from the stem, leaves, and fruit parts of the pineapple (Ananas comosus) and other species of the Bromeliaceae family [4,9]. Recently, several studies have evaluated the effects of deproteinization with papain and bromelain enzymes on the improvement of the shear bond strength of restorative materials to dental structure [4,5,[7][8][9][10][11]. Accordingly, the objective of the current study was to investigate the effects of 10% papain and 6% bromelain enzymes on the shear bond strength (SBS) of composite resin to enamel.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of the Effects of Bromelain and Papain Enzymes on Shear Bond Strength of Composite Resin to Enamel

Sharafeddin

Yazdanpanah

Jowkar

2021

International Journal of Dentistry

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Aim. This study aimed to evaluate the effects of 6% bromelain and 10% papain enzymes on shear bond strength (SBS) of composite resin to enamel compared to conventional 37% phosphoric acid etching. Materials and Methods. 50 human maxillary premolar teeth were randomly divided into 5 groups (G1–G5/n = 10). In G1 and G2, after etching enamel with 37% phosphoric acid for 15 seconds and washing the surface, 10% papain and 6% bromelain enzymes were used, respectively. In G3 and G4, 6% bromelain or 10% papain enzymes were applied on enamel. In G5, the enamel surface was etched with 37% phosphoric acid for 15 seconds. A two-step etch-and-rinse adhesive system (Adper Single Bond 2) was applied. A nanohybrid composite (Z350) was placed using Teflon molds. All the samples were then subjected to the SBS test using a universal testing machine. Data analysis was performed using a one-way ANOVA test followed by the Tukey test. p values less than 0.05 were considered significant. Results. Comparison of the mean SBS between G1, G2, and G5 shows no significant differences ( p > 0.05 ); however, they had higher mean SBS compared with G3 and G4 ( p < 0.0001 ). Conclusions. The shear bond strength of composite to enamel was not affected significantly using either 6% bromelain or 10% papain enzymes after 37% phosphoric acid application. Moreover, 6% bromelain and 10% papain enzymes were not as effective as 37% phosphoric acid alone.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 85%

Section: Introductionmentioning

confidence: 99%

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Evaluation of the Effects of Bromelain and Papain Enzymes on Shear Bond Strength of Composite Resin to Enamel

Sharafeddin

Yazdanpanah

Jowkar

2021

International Journal of Dentistry

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“…In another study, removal of unsupported collagen fiber with bromelain enzyme after acid etching improved the SBS. 4 Hasija et al 24 reported that deproteinization conducted with bromelain exhibited effective bond strength as compared to papain, but it was not statistically significant. However, in our study, there were no significant differences between the specimens exposed to bromelain enzyme before two adhesive systems, which could be attributed to the different step of applying deproteinization agent as we applied it before etching.…”

Section: Resultsmentioning

confidence: 99%

Effect of Papain and Bromelain Enzymes on Shear Bond Strength of Composite to Superficial Dentin in Different Adhesive Systems

Sharafeddin¹,

Safari²

2019

The Journal of Contemporary Dental Practice

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Aim:The aim of this study was to compare different dentin treatments and adhesive systems on shear bond strength (SBS) of composite to superficial dentin. Materials and methods: In this in vitro experimental study, 60 extracted human upper premolars were cut 0.5 mm deep under occlusal DEJ and were randomly divided into six groups (n = 10) based on the method of dentin treatment and adhesive system: (A) two steps self-etch adhesive system (Clearfil SE bond) and (B) two steps of total etch bonding system (Adper single bond 2). (C) 10% papain + Clearfil SE bond. (D) 10% papain + Adper single bond 2. (E) 6% bromelain + Clearfil SE bond. (F) 6% bromelain + Adper single bond 2. Then, a plastic mold was placed on dentin and filled with resin composite. Shear bond strength (MPa) was tested by a universal testing machine (Zwick/Roell Z020, Germany), and the data were statistically analyzed by two-way ANOVA and Tukey's test (p < 0.05) and were applied for mean comparison. Results: A significant difference was detected in the SBS evaluation between the applied adhesive systems (p = 0.0007). The SBS was also significantly affected by the interaction of treatment and etching techniques (p = 0.028). The group with 10% papain as a deproteinizing agent before the self-etching adhesive system showed the highest SBS (p = 0.029). There were no significant differences considering the specimens exposed to papain before the total etch adhesive system (p = 0.13), and the specimens were exposed to bromelain enzyme before self-etch and total etch adhesive systems (p = 0.25, p = 0.84, respectively). Conclusion: Papain enzyme treatment with the self-etch adhesive system increased the SBS value. Additionally, the application of bromelain enzyme as dentin treatment before two adhesive systems and papain before total etch adhesive system had no effect on the SBS of composite to superficial dentin. Clinical significance: Application of adhesive systems and dentin treatment can influence the bond strength.

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“…e225 In a previous study by Espinosa et al (12), the effect of 5.25% sodium hypochlorite applied for 30 and 60 seconds prior to acid etching on the topographical features of the enamel surface was investigated by scanning electron microscopy (SEM) analysis, and it was reported that the quality of the etching pattern was superiorly enhanced after the 60-second application. Other studies have similarly used the deproteinization duration of 60 seconds (9,11,16,20,21). Therefore, in the present study, both deproteinizing agents were applied for 60 seconds.…”

Section: Discussionmentioning

confidence: 99%

Assessment of shear bond strength and microleakage of fissure sealant following enamel deproteinization: An in vitro study

Bayrak

Gurdogan-Guler²,

Yildirim

et al. 2020

J Clin Exp Dent

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Background: To evaluate the effect of two different deproteinizing agents (5.25% sodium hypochlorite and Papaca-rie®) prior to acid etching on the shear bond strength and microleakage of a fissure sealant material. Material and Methods: A total of 123 extracted noncarious human third molar teeth were randomly divided into three groups for each test: acid etching alone (control) (C), sodium hypochlorite (NaOCl), and Papacarie Duo ® (PC). The shear bond strength tests were performed using a universal testing machine, and microleakage was evaluated using a dye penetration method. Results: The mean shear bond strength values obtained in Group C were significantly lower than those of Group NaOCl and Group PC (p=0.0001). There were no significant differences between Group NaOCl and Group PC (p >0.05). No statistically significant difference was observed among the groups in terms of microleakage (p>0.05). Conclusions: Papacarie Duo® and 5.25% sodium hypochlorite treatments before etching increased the shear bond strength of the fissure sealant. However, pre-treatment with these agents did not decrease the microleakage between teeth and sealant.

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Deproteinizing Agents as an Effective Enamel Bond Enhancer-An in Vitro Study

Abstract: Among deproteinizing agents, deproteinization when carried out with bromelain gel and sodium hypochlorite showed effective bond strength as compared to papain.

Cited by 14 publications

References 1 publication

Evaluation of the Effects of Bromelain and Papain Enzymes on Shear Bond Strength of Composite Resin to Enamel

Evaluation of the Effects of Bromelain and Papain Enzymes on Shear Bond Strength of Composite Resin to Enamel

Effect of Papain and Bromelain Enzymes on Shear Bond Strength of Composite to Superficial Dentin in Different Adhesive Systems

Assessment of shear bond strength and microleakage of fissure sealant following enamel deproteinization: An in vitro study

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