Depression of the T Cell Phenomenon of Contact Sensitivity by T Cells from Unresponsive Mice

Zembala, Marek; Asherson, G. L.

doi:10.1038/244227a0

Cited by 163 publications

(48 citation statements)

References 3 publications

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“…These results indicate that immunization with a relatively high dose of carrier antigen generated a subpopulation of T cells that specifically inhibits the antibody response against hapten on the same carrier. Similar antigen-specific T-cell-mediated suppression has recently been demonstrated in a number of other experimental systems (3)(4)(5)(6)(7)(8)(9)(10), and is now considered to be an important regulatory mechanism in various forms of immune responses (reviewed in 11).…”

mentioning

confidence: 87%

Properties of antigen-specific suppressive T-cell factor in the regulation of antibody response of the mouse. I. In vivo activity and immunochemical characterization.

Takemori¹,

Tada²

1975

The Journal of Experimental Medicine

170

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Our previous publications (1, 2) indicated that thymocytes and spleen cells of mice that had been immunized with a high dose of carrier antigen, when transferred into syngeneic host, could suppress the antibody response against a hapten coupled to the homologous carrier . This suppressive effect was found to be mediated by a population of thymus-derived lymphocytes, whose activity was completely abrogated by the treatment with anti-B and complement . Moreover, the specificity of the observed suppression has been established by the fact that neither normal T cells nor those obtained from mice immunized with an unrelated antigen could suppress the antibody response . These results indicate that immunization with a relatively high dose of carrier antigen generated a subpopulation of T cells that specifically inhibits the antibody response against hapten on the same carrier. Similar antigen-specific T-cell-mediated suppression has recently been demonstrated in a number of other experimental systems (3-10), and is now considered to be an important regulatory mechanism in various forms of immune responses (reviewed in 11).A possible molecular mechanism of the antigen-specific suppression is that certain primed T cells liberate a suppressive factor(s) which then gives rise to an "off" signal to other cell types via combination with antigen. There are several examples of soluble T-cell factors that influence the magnitude and quality of the immune response (12-24). Thus, the above findings prompted us to explore the subcellular component of T cells which can mediate the antigen-specific suppression of the antibody response . We have attempted to separate such a soluble component by simple sonication followed by ultracentrifugation of the thymocytes and spleen cells possessing the suppressor activity, since we were successful in obtaining an antigen-specific suppressive factor from sonicated Tcells of the rat, which had shown a strong inhibitory effect on an ongoing IgE antibody response of the same species (19,25) . This paper will describe some of the properties of the antigen-specific suppressive T-cell factor obtained in mice, which was found to suppress mainly IgG antibody response of syngeneic mice in an in vivo experimental system .

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mentioning

confidence: 87%

Properties of antigen-specific suppressive T-cell factor in the regulation of antibody response of the mouse. I. In vivo activity and immunochemical characterization.

Takemori¹,

Tada²

1975

The Journal of Experimental Medicine

170

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“…The positive helper effects on antibody production were recognized first (1,2). Later, suppressor functions were demonstrated in humoral immune responses (3)(4)(5) and more recently in delayed hypersensitivity (6) and the mixed lymphocyte reaction (MLR) (7).' It is now clear from the work of Cantor and Boyse (8) that there are two types of regulatory T lymphocytes, helper and suppressor cells, which belong to separate populations distinguishable by their Ly surface antigens.…”

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confidence: 99%

A suppressor T cell in the human mixed lymphocyte reaction.

McMichael¹,

Sasazuki²

1977

The Journal of Experimental Medicine

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Lymphocytes from an HLA-B7 DW2 homozygous multiparous woman, J.H., failed to respond in the mixed lymphocyte reaction to lymphocytes from her DW1 homozygous husband, W.H., and certain other homozygous typing cells. J.H. lymphocytes could suppress the response of HLA matched responders to W.H. This effect was shown to be radiosensitive and due to a T cell. The suppressor cell showed antigen specificity.

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“…The model we studied was the in vitro production of killer cells specific for trinitrophenyl (TNP) modified selfantigens. Mice that are rendered tolerant to picryl chloride contact hypersensitivity by intravenous injection of trinitrobenzenesulfonic acid (TNBS) (10) or of TNP-labeled syngeneic cells (11)(12)(13) develop suppressor T cells that can interfere with the adoptive transfer of contact hypersensitivity to nonimmune recipients (10) or with the. generation of antigenspecific helper cells (12,13).…”

mentioning

confidence: 99%

T-cell suppression and contrasuppression induced by histamine H2 and H1 receptor agonists, respectively.

Siegel¹,

Schwartz²,

Askenase³

et al. 1982

Proc. Natl. Acad. Sci. U.S.A.

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The intensity of Lyl T helper and delayed type hypersensitivity effector cell activities is governed, in part, by an interplay between two classes of immunoregulatory T cells: suppressor cells and contrasuppressor cells. We asked whether histamine, at concentrations and duration of exposure that we calculated might be achieved at local sites of inflammation, could activate either or both of these classes of regulatory cells in vitro.To answer this question we used spleen cells from mice treated in vivo with the toleragen trinitrobenzenesulfonic acid as regulators of in vitro generation of primary anti-trinitrophenyl self-cytotoxic T lymphocytes. Under the conditions used, these spleen cells had no major regulatory effects. However, if these cells were preincubated with histamine at 0.1 mM for 30-60 min, suppressor activity was induced, but this occurred inconsistently and with nonstoichiometric results. The use of synthetic histamine agonists revealed that histamine may activate both suppressor and contrasuppressor cell subsets. A histamine HI receptor agonist [2-(2-pyridyl)-ethylamine dihydrochloride] had a propensity to activate contrasuppression, whereas an H2 receptor. agonist (dimaprit) tended to activate suppressor cells. Thus, histamine may have opposing actions that obscure suppression. This duality was shown by treatment ofpyridylethylamine-induced contrasuppressor cells with complement and anti-I-J antibody that kills contrasuppressor cells. This treatment revealed a high level of suppressor cell activity that was not expressed until the opposing contrasuppressor cells were removed. Because histamine is released at local sites of delayed type hypersensitivity, these results indicate that histamine may serve as an inducer of microenvironmental immunomodulation by activating regulatory T cells at sites where immune responses are taking place.

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Depression of the T Cell Phenomenon of Contact Sensitivity by T Cells from Unresponsive Mice

Cited by 163 publications

References 3 publications

Properties of antigen-specific suppressive T-cell factor in the regulation of antibody response of the mouse. I. In vivo activity and immunochemical characterization.

Properties of antigen-specific suppressive T-cell factor in the regulation of antibody response of the mouse. I. In vivo activity and immunochemical characterization.

A suppressor T cell in the human mixed lymphocyte reaction.

T-cell suppression and contrasuppression induced by histamine H2 and H1 receptor agonists, respectively.

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