Deposition of silver nanoparticles on titanium surface for antibacterial effect

Liao, Juan; Zhu, Zhiliang; Mo, Anchun; Li, Lei; Zhang, Jingchao

doi:10.2147/ijn.s8810

Cited by 148 publications

(81 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…[62][63][64] Other studies have shown that oxidizing species in lieu of reducing agents might be responsible for the antibacterial effects. 65,66 In our study, the extremely low silver concentrations in the solution ruled out the inherent toxicity of silver ions (Ag + ) to bacteria and so the inhibition to bacteria was mainly due to the neutral metallic silver (Ag 0 ) rendered by Ag NPs.…”

mentioning

confidence: 99%

Hierarchical micro/nanostructured titanium with balanced actions to bacterial and mammalian cells for dental implants

Zhu

Cao

Qiao

et al. 2015

IJN

View full text Add to dashboard Cite

A versatile strategy to endow dental implants with long-term antibacterial ability without compromising the cytocompatibility is highly desirable to combat implant-related infection. Silver nanoparticles (Ag NPs) have been utilized as a highly effective and broad-spectrum antibacterial agent for surface modification of biomedical devices. However, the high mobility and subsequent hazardous effects of the particles on mammalian cells may limit its practical applications. Thus, Ag NPs were immobilized on the surface of sand-blasted, large grit, and acid-etched (SLA) titanium by manipulating the atomic-scale heating effect of silver plasma immersion ion implantation. The silver plasma immersion ion implantation-treated SLA surface gave rise to both good antibacterial activity and excellent compatibility with mammalian cells. The antibacterial activity rendered by the immobilized Ag NPs was assessed using Fusobacterium nucleatum and Staphylococcus aureus , commonly suspected pathogens for peri-implant disease. The immobilized Ag NPs offered a good defense against multiple cycles of bacteria attack in both F. nucleatum and S. aureus , and the mechanism was independent of silver release. F. nucleatum showed a higher susceptibility to Ag NPs than S. aureus , which might be explained by the presence of different wall structures. Moreover, the immobilized Ag NPs had no apparent toxic influence on the viability, proliferation, and differentiation of rat bone marrow mesenchymal stem cells. These results demonstrated that good bactericidal activity could be obtained with very small quantities of immobilized Ag NPs, which were not detrimental to the mammalian cells involved in the osseointegration process, and promising for titanium-based dental implants with commercial SLA surfaces.

show abstract

mentioning

confidence: 99%

Hierarchical micro/nanostructured titanium with balanced actions to bacterial and mammalian cells for dental implants

Zhu

Cao

Qiao

et al. 2015

IJN

View full text Add to dashboard Cite

show abstract

“…The Zol test is based on the leaching of silver ions from the surface, the inhibition of the bacterial growth depends on a sufficient concentration of silver ions in the surrounding aqueous environment. 23 Figure 5 shows the Zol test results by DCVC-Ag1, DCVC-Ag2, and DCVC-Ag3 on S. aureus. All the DCVC-Ag samples showed a clear inhibition zone, which indicated the efficient antibacterial ability of these samples.…”

Section: Antibacterial Characteristics Of the Silvered Cvc Filmsmentioning

confidence: 99%

Antimicrobial activity and cytocompatibility of silver nanoparticles coated catheters via a biomimetic surface functionalization strategy

Yang²,

Zhang³

et al. 2015

IJN

View full text Add to dashboard Cite

Catheter-related bloodstream infections are a significant problem in the clinic and may result in a serious infection. Here, we developed a facile and green procedure for buildup of silver nanoparticles (AgNPs) on the central venous catheters (CVCs) surface. Inspired by mussel adhesive proteins, dopamine was used to form a thin polydopamine layer and induce AgNPs formation without additional reductants or stabilizers. The chemical and physicochemical properties of AgNPs coated CVCs were characterized by scanning electron microscopy, X-ray photoelectron spectroscopy, Raman spectroscopy, and water contact angle. The Staphylococcus aureus culture experiment was used to study the antibacterial properties. The cytocompatibility was assessed by water soluble tetrazolium salts (WST-1) assay, fluorescence staining, and scanning electron microscopy analysis. The results indicated that the CVCs surface was successfully coated with compact AgNPs. AgNPs were significantly well separated and spherical with a size of 30–50 nm. The density of AgNPs could be modulated by the concentration of silver nitrate solution. The antibacterial activity was dependent on the AgNPs dose. The high dose of AgNPs showed excellent antibacterial activity while associated with increased cytotoxicity. The appropriate density of AgNPs coated CVCs could exhibit improved biocompatibility and maintained evident sterilization effect. It is promising to design mussel-inspired silver releasing CVCs with both significant antimicrobial efficacy and appropriate biological safety.

show abstract

“…[11][12][13][14] The Ag-nHA-nTiO 2 /PA66 nanocomposite membrane contains 0.48% TiO 2 and 2.35% Ag + . Ag + has been used for many years as a broad spectrum antibacterial agent.…”

Section: Ye Et Almentioning

confidence: 99%

“…The addition of Ag + produces a synergy effect with TiO 2 , resulting in an antimicrobial effect under visible light. 11 Ag-nHA-nTiO 2 / PA66 has been proven to have useful antibacterial properties, especially on Gram-negative bacteria. In our previous study in rats, we found that the osteogenic activity of Ag-nHA-nTiO 2 /PA66 membranes was comparable with that of conventional expanded poly tetrafluroethylene (e-PTFE) membranes and provided a safe strategy for reducing the inflammation response and enhancing bone regeneration, thus promoting the predictability of GBR.…”

Section: Ye Et Almentioning

confidence: 99%

Effects of an antibacterial membrane on osteoblast-like cells in vitro

Yao²,

Mo³

et al. 2011

IJN

View full text Add to dashboard Cite

Infection around membranes is often found in guided bone regeneration (GBR). The excellent antibacterial properties of Ag-nHA-nTiO 2 /polyamide-66 (PA66) nanocomposite membranes have been demonstrated previously. The aim of this study was to observe the microstructure of an Ag-nHA-nTiO 2 /PA66 membrane and its effects on osteoblast-like cells in vitro. An Ag-nHA-nTiO 2 /PA66 membrane was used in the experimental group, and both nHA/PA66 and expanded poly tetrafluroethylene (e-PTFE) membranes were set as control. MG63 osteoblast-like cells were cultured on the three kinds of membrane and tissue culture polystyrene (TCP). The microstructure of the above membranes and the cells adhered on them were detected by scanning electronic microscope (SEM). Cell proliferation was determined by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, cell viability with a cell viability analyzer, and alkaline phosphatase (ALP) activity and Ca 2+ concentration of osteoblast-like cell matrix by enzyme-linked immunosorbent assay. SEM showed that both Ag-nHA-nTiO 2 /PA66 membranes and nHA/PA66 membranes were composed of porous obverse face and smooth opposite face. The e-PTFE membranes showed elliptic surface structure with many tiny lined cracks. The MG63 cells adhered and proliferated well on all three kinds of membranes. Though cell viability on Ag-nHA-nTiO 2 /PA66 membranes was significantly lower than that of the control groups ( P < 0.05), MTT values, ALP activity, and Ca 2+ concentration did not differ significantly among the three kinds of membranes ( P > 0.05). From these findings, it can be concluded that Ag-nHA-nTiO 2 /PA66 membranes are as biocompatible as nHA/ PA66 membranes and TCP, thus may be applied safely in GBR.

show abstract

Deposition of silver nanoparticles on titanium surface for antibacterial effect

Cited by 148 publications

References 20 publications

Hierarchical micro/nanostructured titanium with balanced actions to bacterial and mammalian cells for dental implants

Hierarchical micro/nanostructured titanium with balanced actions to bacterial and mammalian cells for dental implants

Antimicrobial activity and cytocompatibility of silver nanoparticles coated catheters via a biomimetic surface functionalization strategy

Effects of an antibacterial membrane on osteoblast-like cells in vitro

Contact Info

Product

Resources

About