Deposition of Retrovirus Associated Antigens (p30 and gp70) on Cell Membranes of Feline and Murine Leukaemia Virus Infected Cells

O’Brien, Stephen J.; Simonson, Janice M.; Davis, Stephen D.

doi:10.1099/0022-1317-38-3-483

Cited by 5 publications

(3 citation statements)

References 33 publications

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“…To detect the presence of Rauscher mouse leukemia virus (MuLV) p30, tissues from 16 SJCRH and 9 CRE mice were stained. The goat anti-p30 antibody was provided by Dr. Sandra Ruscetti, the National Cancer Institute, Frederick, Maryland (O’Brien, Simonson, and Davis 1978), and IHC p30 procedures were as described previously (Hartley et al 2008).…”

Section: Methodsmentioning

confidence: 99%

Immmunophenotype of Spontaneous Hematolymphoid Tumors Occurring in Young and Aging Female CD-1 Mice

et al. 2015

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A few reports indicated the incidence of hematolymphoid neoplasms in old CD-1 mice, but the cellular lineage of CD-1 mouse neoplasms has not be published. In this study, immunohistochemistry (IHC) was used to characterize the cellular lineage of spontaneous hematolymphoid neoplasms arising in young female CD-1 mice used as health monitoring sentinels and aging female CD-1 mice used as controls in 80 wk carcinogenesis studies. Lymphoblastic lymphomas of T-cell and B-cell lineage were common in mice 12 mo or less of age, whereas a wide range of non-lymphoblastic B-cell lymphomas and lymphoblastic T-cell lymphomas were common in mice > 12 mo old. Renal hyaline droplets positive for lysozyme were observed in aged mice with a histiocytic-associated large B-cell lymphoma (HA-BCL) and a myeloid leukemia. Endogenous ecotropic MuLV genes have been recovered from CD-1 mice, but MuLV protein expression has not been previously demonstrated. We reported for the first time the expression of MuLV protein by IHC in lymphomas and some normal tissues of both young and aging CD-1 mice. This report should help to differentiate spontaneous lymphomas and leukemias in CD-1 mice from those induced by chemicals and other methods.

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Section: Methodsmentioning

confidence: 99%

Immmunophenotype of Spontaneous Hematolymphoid Tumors Occurring in Young and Aging Female CD-1 Mice

et al. 2015

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“…The p30 antibody has been previously published as being cross-reactive with endogenous retroviruses, specifically endogenous murine leukemia viruses. 23,46,53 CD1 mouse tissues were used as a positive control for interpreting p30 labeling. 53 C57BL/6 (B6) mouse tissues were used as negative controls for the p30 antibody since B6 mice have been reported to have undetectable ecotropic MuLV.…”

Section: Methodsmentioning

confidence: 99%

“…58 In this study, we have immunophenotyped 12 lymphomas arising spontaneously in NSG mice and found that select nontumor tissues of all NSG mice were positive for the retroviral p30 core protein, which has been shown to cross-react with endogenous murine leukemia viruses. 46 Most of the lymphomas were also strongly immunopositive for p30. We discuss the implications of these observations in relation to other immunocompromised mouse strains and for research studies involving NSG mice.…”

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confidence: 95%

Morphologic and Immunohistochemical Characterization of Spontaneous Lymphoma/Leukemia in NSG Mice

et al. 2019

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The NOD.Cg- Prkdcscid Il2rgtm1Wjl/SzJ strain (NOD scid gamma, NSG) is a severely immunodeficient inbred laboratory mouse used for preclinical studies because it is amenable to engraftment with human cells. Combining scid and Il2rgnull mutations results in severe immunodeficiency by impairing the maturation, survival, and functionality of interleukin 2–dependent immune cells, including T, B, and natural killer lymphocytes. While NSG mice are reportedly resistant to developing spontaneous lymphomas/leukemias, there are reports of hematopoietic cancers developing. In this study, we characterized the immunophenotype of spontaneous lymphoma/leukemia in 12 NSG mice (20 to 38 weeks old). The mice had a combination of grossly enlarged thymus, spleen, or lymph nodes and variable histologic involvement of the bone marrow and other tissues. All 12 lymphomas were diffusely CD3, TDT, and CD4 positive, and 11 of 12 were also positive for CD8, which together was consistent with precursor T-cell lymphoblastic lymphoma/leukemia (pre-T-LBL). A subset of NSG tissues from all mice and neoplastic lymphocytes from 8 of 12 cases had strong immunoreactivity for retroviral p30 core protein, suggesting an association with a viral infection. These data highlight that NSG mice may develop T-cell lymphoma at low frequency, necessitating the recognition of this spontaneously arising disease when interpreting studies.

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A molecular approach to the identification and individualization of human and animal cells in culture: Isozyme and allozyme genetic signatures

1980

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The electrophoretic resolution of a group of genetically monomorphic gene-enzyme systems that are developmentally and biologically ubiquitous has been used to provide a species-specific and type-specific biochemical characterization of various cultured cells. The relative mobilities of gene-enzyme systems representing nine distinct gene products from cell cultures of 25 species from Drosophila to man are presented. These isoenzymes effectively discriminate interspecies cell-to-cell contamination and almost invariably serve to identify the contaminating species. The resolution of eight polymorphic gene-enzyme systems in human cell cultures provides a virtually unique allozyme genetic signature as a monitor of intraspecies cellular contamination. The genetic signatures of 47 commonly used human cells are presented. Included in the test were seven putative HeLa (human cervical carcinoma) contaminants each of which expressed a signature identical with that of HeLa. The probability that an unrelated human cell line will have a signature identical to a typed cell is computed for each line from the genotypic frequencies at each locus in a population of cultured human cells. The gene frequencies of this cell population are comparable to the same frequencies in natural human populations. The most common human signature has a frequency (and therefore a probability) of 0.02. The majority of the 17,010 possible signatures are far less probable. A calculation of the theoretical incidence of chance matching of signatures within test groups of two or more individuals is presented. The probability of a chance match between any two randomly selected individuals is 0.004 and among five randomly selected individuals is 0.034. The allozyme genetic signature represents a definitive monitor of cell identity and is presented as a standard of cell and tissue identification for a variety of biological studies.

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Deposition of Retrovirus Associated Antigens (p30 and gp70) on Cell Membranes of Feline and Murine Leukaemia Virus Infected Cells

Cited by 5 publications

References 33 publications

Immmunophenotype of Spontaneous Hematolymphoid Tumors Occurring in Young and Aging Female CD-1 Mice

Immmunophenotype of Spontaneous Hematolymphoid Tumors Occurring in Young and Aging Female CD-1 Mice

Morphologic and Immunohistochemical Characterization of Spontaneous Lymphoma/Leukemia in NSG Mice

A molecular approach to the identification and individualization of human and animal cells in culture: Isozyme and allozyme genetic signatures

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