Deposition of Platinum Clusters on Surface-Modified Tobacco Mosaic Virus

Abstract

Nanoscaled Pt conductors were prepared from genetically engineered Tobacco mosaic virus (TMV) templates through Pt cluster deposition on the outer surface of the TMV. Pt clusters were synthesized and deposited on the engineered TMV with surface-exposed cysteine via the in situ mineralization of hexachloroplatinate anions. This deposition was driven by the specific binding between thiols and the solid metal clusters. In addition, Pt-thiolate adducts are suggested to form on the engineered TMV in aqueous solutions that work as nucleation sites for the formation of the Pt clusters. The specific binding between Pt clusters and the engineered TMV template was investigated using UV/vis spectrophotometry and quartz crystal microbalance (QCM) analysis. The electric conductance of Pt-deposited TMV was greater than that of the uncoated TMV virion particles. This result suggests the application of metal cluster-deposited engineered TMV in future electrical devices such as rapid response sensors.

Preparation of silica stabilized Tobacco mosaic virus templates for the production of metal and layered nanoparticles

“…Inorganic deposition onto these bio-templates has been accomplished using a variety of methodologies including chemical crosslinking, genetic engineering, and electroless plating, resulting in the deposition of numerous inorganic compounds including metal particles, silica, metal oxides, and metal sulfides [7][8][9][10]. Virusassembled inorganic nanostructures have been fashioned into conductive nanowires, field effect transistors, memory device components, and battery electrodes [11][12][13][14]. From these studies it is clear that inherent biologically properties of viruses, includ-* Corresponding author.…”

“…Coating of materials onto the TMV surface has relied on electrostatic interactions in aqueous solvents [8,12,17,18]. In these instances, the solution pH was adjusted so the charge of the coating particle and that of the biological template were mutually attractive.…”

The promotion of osteoblastic differentiation of rat bone marrow stromal cells by a polyvalent plant mosaic virus

“…The symmetrical arrangement of the outer coat proteins of the viral particles makes them attractive scaffolds for polyvalent display of a variety of functional groups for various applications [32][33][34][35]. In the past two decades, taking advantages of distinctive chemical and biological properties of plant viruses, new materials with unique structural features have been developed for a wide range of applications, including electronics, drug delivery, imaging, gene therapy, and immunotherapy [35][36][37][38][39][40][41][42][43][44][45][46][47]. Recent studies have reported that cowpea mosaic virus (CPMV) can interact with the mammalian cells due to the presence of two highly specific CPMV-binding proteins present in certain mammalian cells [48].…”

Palladium nanoparticles supported by amyloid fibrils: From size controllable synthesis to extremely high catalytic performance

“…3c shows that small Pd NPs are highly crystalline with spacings of 0.23 nm, which closely matches the (1 1 1) interplanar distance of FCC palladium metal [32]. The formation of Pd-insulin complexes is likely because insulin peptides contain several Cys-rich regions that are known to enhancing the "biosorption" [33,34]. Moreover, carboxyl and amino acid side chains such as serine are also known to have strong complexation abilities with metal precursors.…”