Deposition and Retention of Ultrafine Aerosol Particles in the Human Respiratory System. Normal and Pathological Cases

Gradoń, Leon; Orlicki, Dariusz; Podgórski, Albert

doi:10.1080/10803548.2000.11076451

Cited by 15 publications

(9 citation statements)

References 13 publications

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“…During various processes, workers are exposed to various sizes of WC-Co particles including nanoparticles [32]. Recent evidence indicates that nanoparticles exhibit a higher deposition in all regions of the respiratory tract when compared to the fine particles, and that exposure to nanoparticles may induce cytotoxicity, pulmonary inflammation, and/or other adverse effects in the cells and lung [3]. However, the molecular mechanisms by which WC-Co exposure causes lung injury and carcinogenesis remain to be clarified.…”

Section: Discussionmentioning

confidence: 97%

“…There are increasing public concerns over the adverse health effects of exposure to nanoparticles and possible environmental impact of this emerging nanotechnology. Recent evidence has indicated that nanoparticles exhibit a higher deposition in all regions of the respiratory tract when compared to the fine particles, and that exposure to nanoparticles may induce cytotoxicity, pulmonary inflammation, and/or other adverse effects in the cells and the lung [2][3][4][5]. Once deposited in the airways, nanoparticles may migrate more rapidly to the interstitium and lymph nodes of the lung than the fine particles [6].…”

Section: Introductionmentioning

confidence: 95%

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Tungsten Carbide-Cobalt Nanoparticles Induce Reactive Oxygen Species, AKT, ERK, AP-1, NF-κB, VEGF, and Angiogenesis

Liu

Ding

Zheng

et al. 2015

Biol Trace Elem Res

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Powder mixtures of tungsten carbide and metallic cobalt (WC-Co) are widely used in various products. Nanoparticles are engineered structures with at least one dimension of 100 nm or smaller. WC-Co is known to be associated with lung injury and diseases. Angiogenesis is a key process during vasculature, carcinogenesis, recovery of injury, and inflammatory diseases. However, the cellular effects of WC-Co nanoparticles on angiogenesis remain to be elucidated. In this study, we investigated angiogenic response and relative mechanisms after exposure to WC-Co nanoparticles. Our results showed that WC-Co nanoparticles at 5 μg/cm(2) induced ROS production which activated AKT and ERK1/2 signaling pathways in lung epithelial cells by reactive oxygen species (ROS) staining and immunoblotting; WC-Co treatment also increased transcriptional activation of AP-1, NF-κB, and VEGF by reporter assay. Further studies demonstrated that ROS are upstream molecules of AKT and ERK signaling pathways; the activation of AP-1, NF-κB, and VEGF was through ROS generation, AKT and ERK1/2 activation. In addition, WC-Co nanoparticles affected the cells to induce angiogenesis by chicken chorioallantoic membrane (CAM) assay. These results illustrate that exposure to WC-Co nanoparticles induces angiogenic response by activating ROS, AKT, and ERK1/2 signaling pathways and the downstream molecules and elucidate the potential molecular mechanisms during this process. This information may be useful for preventing potential damage from nanoparticle exposure in the future.

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Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 95%

Tungsten Carbide-Cobalt Nanoparticles Induce Reactive Oxygen Species, AKT, ERK, AP-1, NF-κB, VEGF, and Angiogenesis

Liu

Ding

Zheng

et al. 2015

Biol Trace Elem Res

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“…Токсикологические и эпидемиологические иссле дования базируются на предположениях о том, что причиной изменений в состоянии здоровья работаю щих могут быть ультрадисперсные частицы, посту пающие в организм ингаляционным путем [19,29]. Несмотря на то, что токсикологические механизмы таких эффектов недостаточно изучены, очевидно, что по сравнению с более крупными частицами, уль трамалые частицы обладают большей биологичес кой активностью и могут индуцировать интенсивные воспалительные реакции [16,30].…”

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“…На основании полу ченных данных было высказано предположение о су ществовании связи между отложением и задержкой наночастиц в легких с патологическими изменения ми легочной ткани (фиброз и ухудшение эластич ности). Рядом исследователей высказывается пред положение о том, что если воздействию ультрадис персных частиц предшествовала длительная экспо зиция частицами более крупного размера, обусло вившая возникновение патологических изменений легочной ткани, то ультрадисперсные частицы могут скапливаться в альвеолах, способствуя, таким обра зом, развитию эмфиземы легких [19,29,31 ].…”

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Nanoparticles: possible contribution to development of occupational pathology

Demetska¹,

Kucheruk²,

Movchan³

2006

Ukr. ž. probl. med. pr.

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Л е м е и к а я A .B ., К у н е р у к Т . К . , М о в ч а н В .А .Институт медицины труда АМ Н Украины, г. Киев Нанотехнологическая революция стала причиной нового витка в развитии машиностроения, информационных тех нологий, медицинской диагностики и обусловила появление новой «субкатегории» токсикологии -нанотоксиколо гии. Предполагается, что наночастицы, присутствующие в воздухе рабочей зоны, могут способствовать повышению заболеваемости и смертности от патологии дыхательной и сердечно-сосудистой систем вследствие специфической токсикологической роли ультрадисперсных частиц. Изучение биологических свойств и роли наночастиц в развитии профессионально обусловленной патологии является одной из актуальных проблем как в мире, так и в Украине.

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“…During various processes, workers are exposed to various particle sizes of WC-Co heavy metal including nanoparticles. Recent evidence indicates that nanoparticles exhibit a higher deposition in all regions of the respiratory tract when compared to the fine particles, and that exposure to nanoparticles may induce cytotoxicity, pulmonary inflammation, and/or other adverse effects in the cells and lung 36 . However, the molecular mechanisms by which WC-Co exposure causes lung injury and carcinogenesis remain to be clarified.…”

Section: Vegf and Angiogenesismentioning

confidence: 99%

Mechanisms of Tungsten Carbide-Cobalt Nanoparticle-induced Angiogenesis

Zhu¹

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Mechanisms of Tungsten Carbide-Cobalt Nanoparticle-induced Angiogenesis Yingxue Zhu Hard metal or cemented carbide consists of a powder mixture of 80 to 90% of tungsten carbide (WC) and 5 to 10% of metallic cobalt (Co). Nanoparticles are engineered structures with one dimension of at least 100 nanometers or smaller. The small size results in properties that may be substantially different from particles of the same composition in the micrometer scale. It has been demonstrated that the inhalation of hard metal particles may cause an interstitial pulmonary disease and lung cancer, the mechanism of which involves an interaction between Co and WC particles, the generation of reactive oxygen species, and oxidant-induced DNA-damage (1,2,3,4). However, the effect of WC-Co on biologic responses remains to be elucidated. In this study, we analyzed the effects of WC-Co nanoparticles in regulating production of reactive oxygen species (ROS), activation of AP-1, NF-κB, VEGF, AKT or ERK, and angiogenesis. We also tested the effect of fine-and nano-sizes of WC-Co with equal surface area on ROS production and the activation of AKT and ERK. Results show that WC-Co nanoparticles at 5 µg/cm2 induce ROS production which activates AKT and ERK signaling pathways in lung epithelial cells. ROS also increased transcriptional activation of AP-1, NF-κB, as well as VEGF (via AKT and ERK1/2 activation); while the fine size of WC-Co particles at the same concentration did not have significant effect. However, at high concentrations, fine-size WC-Co particles also induced ROS production, and AKT and ERK1/2 activation. WC-Co nanoparticles also induced angiogenesis in cells. These results identify multiple signaling pathways that are activated by WC-Co nanoparticle treatment, and elucidate the potential molecular mechanisms of their effects on the cells. This information may be useful for preventing potential damage from WC-Co particle exposure in the future.

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Deposition and Retention of Ultrafine Aerosol Particles in the Human Respiratory System. Normal and Pathological Cases

Cited by 15 publications

References 13 publications

Tungsten Carbide-Cobalt Nanoparticles Induce Reactive Oxygen Species, AKT, ERK, AP-1, NF-κB, VEGF, and Angiogenesis

Tungsten Carbide-Cobalt Nanoparticles Induce Reactive Oxygen Species, AKT, ERK, AP-1, NF-κB, VEGF, and Angiogenesis

Nanoparticles: possible contribution to development of occupational pathology

Mechanisms of Tungsten Carbide-Cobalt Nanoparticle-induced Angiogenesis

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