Depolymerisation products of hyaluronic acid after exposure to oxygen-derived free radicals.

McNeil, Julian; Wiebkin, Ole W.; Betts, W H; Cleland, Leslie G.

doi:10.1136/ard.44.11.780

Cited by 92 publications

(49 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Infants of HIV seropositive mothers are almost always seropositive at birth but might during the first 18 months of life turn seronegative if they are not infected (44) (49), and that this depolymerization is induced by oxygen free radicals (58,59), again fits in the previous hypothesis. This same approach, i.e., the evaluation of tissue LPO products levels in tissues, has been also applied to experimental models with similar results.…”

Section: Semm Malondialdehyde In Patientssupporting

confidence: 70%

Lipid Peroxidation Products and Antioxidants in Human Disease

Romero¹,

Bosch‐Morell²,

Romero³

et al. 1998

Environmental Health Perspectives

View full text Add to dashboard Cite

Lipid peroxidation (LPO) is a free radical-related process that in biologic systems may occur under enzymatic control, e.g., for the generation of lipid-derived inflammatory mediators, or nonenzymatically. This latter form is associated mostly with cellular damage as a result of oxidative stress, which also involves cellular antioxidants in this process. This article focuses on the relevance of two LPO products, malondialdehyde (MDA) and 4-hydroxynonenal (HNE), to the pathophysiology of human disease. The former has been studied in human serum samples of hepatitis C virus-infected adults and human immunodeficiency virus-infected children. In these two cases it is shown that the specific assay of serum MDA is useful for the clinical management of these patients. The presence of MDA in subretinal fluid of patients with retinal detachment suggests the involvement of oxidative stress in this process. Moreover, we were able to report the dependence of this involvement on the degree of myopia in these patients. The assay of MDA contents in the peripheral nerves of rats fed a chronic alcohol-containing diet or diabetic mice also confirms the pathophysiologic role of oxidative stress in these experimental models. In these two cases, associated with an increase in tissue LPO products content, we detected a decrease of glutathione peroxidase (GSHPx) activity in peripheral nerve, among other modifications. We have demonstrated that in vitro HNE is able to inhibit GSHPx activity in an apparent competitive manner, and that glutathione may partially protect and/or prevent this inactivation. The accumulation of LPO products in the brain of patients with Alzheimer's disease has also been described, and it is on the basis of this observation that we have tried to elucidate the role of oxidative stress and cellular antioxidants in ,B-amyloid-induced apoptotic cell death of rat embryo neurons. Finally, we discuss the possible role of the observed vascular effects of HNE on human arteries.

show abstract

Section: Semm Malondialdehyde In Patientssupporting

confidence: 70%

Lipid Peroxidation Products and Antioxidants in Human Disease

Romero¹,

Bosch‐Morell²,

Romero³

et al. 1998

Environmental Health Perspectives

View full text Add to dashboard Cite

show abstract

“…HA oligomers, and thus HA-degradation products, can have pro-or anti-inflammatory effects depending on their molecular weight [72][73][74]. Actually, different reactive oxygen species involved in the degradation process lead to different chemically modified HA oligomers, which are the ones involved in inflammation [75,76]. Low molecular weight fragments of HA tend to be pro-inflammatory, whereas high-molecular weight fragments have anti-inflammatory effects [77].…”

Section: Discussionmentioning

confidence: 99%

Schwann-cell cylinders grown inside hyaluronic-acid tubular scaffolds with gradient porosity

et al. 2016

View full text Add to dashboard Cite

Cell transplantation therapies in the nervous system are frequently hampered by glial scarring and cell drain from the damaged site, among others. To improve this situation, new biomaterials may be of help. Here, novel single-channel tubular conduits based on hyaluronic acid (HA) with and without poly-L-lactide acid fibers in their lumen were fabricated. Rat Schwann cells were seeded within the conduits and cultured for 10 days.The conduits possessed a three-layered porous structure that impeded the leakage of the cells seeded in their interior and made them impervious to cell invasion from the exterior, while allowing free transport of nutrients and other molecules needed for cell survival. The channel's surface acted as a template for the formation of a cylindrical sheath-like tapestry of Schwann cells continuously spanning the whole length of the lumen. Schwann-cell tubes having a diameter of around 0.5 mm and variable lengths can thus be generated. This structure is not found in nature and represents a truly engineered tissue, the outcome of the specific cell-material interactions. The conduits might be useful to sustain and protect cells for transplantation, and the biohybrids here described, together with neuronal precursors, might be of help in building bridges across significant distances in the central and peripheral nervous system.

show abstract

“…However, under inflammatory conditions HA has been shown to be more polydisperse, with a preponderance of lower molecular weight forms (4). The accumulation of lower molecular weight forms of HA has been postulated to occur by a variety of mechanisms including depolymerization by reactive oxygen species, enzymatic cleavage, and de novo synthesis of lower molecular weight species (3,(5)(6)(7)(8). Several studies have suggested that high and lower molecular weight HA may exhibit different biological effects on cells and in tissues (9,10).…”

Section: Introductionmentioning

confidence: 99%

Hyaluronan (HA) fragments induce chemokine gene expression in alveolar macrophages. The role of HA size and CD44.

McKee

Penno

Cowman³

et al. 1996

J. Clin. Invest.

732

546

View full text Add to dashboard Cite

Hyaluronan (HA) is a glycosaminoglycan constituent of extracellular matrix. In its native form HA exists as a high molecular weight polymer, but during inflammation lower molecular weight fragments accumulate. We have identified a collection of inflammatory genes induced in macrophages by HA fragments but not by high molecular weight HA. These include several members of the chemokine gene family: macrophage inflammatory protein-1 ␣ , macrophage inflammatory protein-1 ␤ , cytokine responsive gene-2, monocyte chemoattractant protein-1, and regulated on activation, normal T cell expressed and secreted. HA fragments as small as hexamers are capable of inducing expression of these genes in a mouse alveolar macrophage cell line, and monoclonal antibody to the HA receptor CD44 completely blocks binding of fluorescein-labeled HA to these cells and significantly inhibits HA-induced gene expression. We also investigated the ability of HA fragments to induce chemokine gene expression in human alveolar macrophages from patients with idiopathic pulmonary fibrosis and found that interleukin-8 mRNA is markedly induced. These data support the hypothesis that HA fragments generated during inflammation induce the expression of macrophage genes which are important in the development and maintenance of the inflammatory response.

show abstract

Depolymerisation products of hyaluronic acid after exposure to oxygen-derived free radicals.

Cited by 92 publications

References 16 publications

Lipid Peroxidation Products and Antioxidants in Human Disease

Lipid Peroxidation Products and Antioxidants in Human Disease

Schwann-cell cylinders grown inside hyaluronic-acid tubular scaffolds with gradient porosity

Hyaluronan (HA) fragments induce chemokine gene expression in alveolar macrophages. The role of HA size and CD44.

Contact Info

Product

Resources

About