Full-field velocity measurements offered by Particle Imaging Velocimetry (PIV) will represent a major capability enhancement within Australia's only large-scale Transonic Wind Tunnel (TWT). Until now, the application of PIV in Defence Science and Technology (DST) Group's closed-circuit TWT has not been attempted due to the potential risks posed to tunnel subsystems from the deposition of recirculating tracer particles. DST researchers have since examined tracer particle options through literature review, correspondence with operators of other TWT facilities, and pre-installation particle performance assessments conducted in the DST Low Speed Wind Tunnel (LSWT). Simultaneous 2D-PIV and aerosol particle sizing measurements have been collected for the assessment of five different seed materials including: 3 water-based particle mixtures, white mineral oil, and Di-Ethyl-Hexyl-Sebbecat (DEHS).The particle size distributions were then used to estimate particle response for future supersonic application in the TWT.