Depletion of pentachlorophenol in soil microcosms with Byssochlamys nivea and Scopulariopsis brumptii as detoxification agents

Hechmi, Nejla; Bosso, Luciano; El-Bassi, Leila; Scelza, Rosalia; Testa, Antonino; Jedidi, Naceur; Rao, María A.

doi:10.1016/j.chemosphere.2016.09.062

Cited by 39 publications

(17 citation statements)

References 44 publications

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“…The soil slurry method was used to investigate cypermethrin degradation by strain SG4. We placed 50 g autoclaved soil in 250 mL flasks, and 20 mL minimal salt broth was added [37,38]. We then added 1 mL active bacterial inoculum into each flask containing 100 mg•L −1 cypermethrin and the experiment was performed in triplicate at 32 • C and 110 rpm on a rotary shaker.…”

Section: Cypermethrin Degradation In Soil Slurry and Metabolite Identmentioning

confidence: 99%

Enhanced Cypermethrin Degradation Kinetics and Metabolic Pathway in Bacillus thuringiensis Strain SG4

et al. 2020

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Cypermethrin is popularly used as an insecticide in households and agricultural fields, resulting in serious environmental contamination. Rapid and effective techniques that minimize or remove insecticidal residues from the environment are urgently required. However, the currently available cypermethrin-degrading bacterial strains are suboptimal. We aimed to characterize the kinetics and metabolic pathway of highly efficient cypermethrin-degrading Bacillus thuringiensis strain SG4. Strain SG4 effectively degraded cypermethrin under different conditions. The maximum degradation was observed at 32 °C, pH 7.0, and a shaking speed of 110 rpm, and about 80% of the initial dose of cypermethrin (50 mg·L−1) was degraded in minimal salt medium within 15 days. SG4 cells immobilized with sodium alginate provided a higher degradation rate (85.0%) and lower half-life (t1/2) of 5.3 days compared to the 52.9 days of the control. Bioaugmentation of cypermethrin-contaminated soil slurry with strain SG4 significantly enhanced its biodegradation (83.3%). Analysis of the degradation products led to identification of nine metabolites of cypermethrin, which revealed that cypermethrin could be degraded first by cleavage of its ester bond, followed by degradation of the benzene ring, and subsequent metabolism. A new degradation pathway for cypermethrin was proposed based on analysis of the metabolites. We investigated the active role of B. thuringiensis strain SG4 in cypermethrin degradation under various conditions that could be applied in large-scale pollutant treatment.

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Section: Cypermethrin Degradation In Soil Slurry and Metabolite Identmentioning

confidence: 99%

Enhanced Cypermethrin Degradation Kinetics and Metabolic Pathway in Bacillus thuringiensis Strain SG4

et al. 2020

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“…A synergistic effect of compost and fungal strains was observed with a reduction of more than 95% of PCP after 28 days of incubation. The detoxification role of the two fungal strains in the contaminated soil was also confirmed by toxicity assays [89].…”

Section: Microcosm Study At the Lab Scalementioning

confidence: 70%

Mycoremediation in Soil

Bosco¹,

Mollea²

2019

Environmental Chemistry and Recent Pollution Control Approaches

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The chapter reviews the most important researches on the use of micro-and macrofungi in the bioremediation of contaminated soils. In particular, the main classes of soil pollutants in Europe (heavy metals, mineral oils, polycyclic aromatic hydrocarbons (PAHs), monoaromatic hydrocarbons, phenols and chlorinated hydrocarbons (CHCs)), together with the emerging contaminants (i.e. endocrinedisrupting chemicals (EDCs) and pharmaceutical-personal care products (PPCPs)) are considered. A description of the fungal species (saprotrophic and biotrophic basidiomycetes) and biodegradative extracellular (laccases and class II peroxidases) and intracellular (cytochrome P450 monooxygenases and glutathione transferases) enzyme classes is reported. Moreover, the chemical-physical parameters that influence the biodegradation process are examined, and the biostimulation and bioaugmentation strategies are described. A specific attention is paid to the microcosm studies, at the laboratory scale, which are an essential approach to evaluate the feasibility of a biodegradation process.

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“…For example, improvement of halogenated aromatic degradation by simply mixing microbial consortia that have synergetic effects on the degradation of halogenated aromatics has been investigated. Reports on pentachlorophenol and chlorobenzene detoxification by mixtures of microbes showed a higher consumption as compared to a single organism (Hechmi et al ., ; Cheng et al ., ). On the other hand, bioaugmentation through the induction of native strains was also demonstrated to be useful.…”

Section: Technologies Of Halogenated Aromatic‐degrading Microbes and mentioning

confidence: 99%

Microbial degradation of halogenated aromatics: molecular mechanisms and enzymatic reactions

Pimviriyakul

Wongnate

Tinikul

et al. 2019

Microbial Biotechnology

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Summary Halogenated aromatics are used widely in various industrial, agricultural and household applications. However, due to their stability, most of these compounds persist for a long time, leading to accumulation in the environment. Biological degradation of halogenated aromatics provides sustainable, low‐cost and environmentally friendly technologies for removing these toxicants from the environment. This minireview discusses the molecular mechanisms of the enzymatic reactions for degrading halogenated aromatics which naturally occur in various microorganisms. In general, the biodegradation process (especially for aerobic degradation) can be divided into three main steps: upper, middle and lower metabolic pathways which successively convert the toxic halogenated aromatics to common metabolites in cells. The most difficult step in the degradation of halogenated aromatics is the dehalogenation step in the middle pathway. Although a variety of enzymes are involved in the degradation of halogenated aromatics, these various pathways all share the common feature of eventually generating metabolites for utilizing in the energy‐producing metabolic pathways in cells. An in‐depth understanding of how microbes employ various enzymes in biodegradation can lead to the development of new biotechnologies via enzyme/cell/metabolic engineering or synthetic biology for sustainable biodegradation processes.

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Depletion of pentachlorophenol in soil microcosms with Byssochlamys nivea and Scopulariopsis brumptii as detoxification agents

Cited by 39 publications

References 44 publications

Enhanced Cypermethrin Degradation Kinetics and Metabolic Pathway in Bacillus thuringiensis Strain SG4

Enhanced Cypermethrin Degradation Kinetics and Metabolic Pathway in Bacillus thuringiensis Strain SG4

Mycoremediation in Soil

Microbial degradation of halogenated aromatics: molecular mechanisms and enzymatic reactions

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