Depletion of CDC5L inhibits bladder cancer tumorigenesis

Zhang, Ziwei; Mao, Weipu; Wang, Longsheng; Liu, Mengnan; Zhang, Wentao; Wu, Yuan; Zhang, Junfeng; Mao, Shun; Geng, Jiang; Yao, Xudong

doi:10.7150/jca.32850

Cited by 31 publications

(31 citation statements)

References 24 publications

(23 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In bladder cancer, CDC5L expression was obviously increased and CDC5L expression was significantly related to pathology grade and Ki67 expression in bladder cancer. Furthermore, CDC5L inhibition suppressed proliferation, migration, invasion and EMT while induced apoptosis of bladder cancer cells [26]. Here, we have confirmed that CDC5L interference can effectively inhibit the promotion effects on proliferation and cell cycle induced by ANXA7 overexpression.…”

Section: Discussionsupporting

confidence: 70%

ANXA7 promotes the cell cycle, proliferation and cell adhesion-mediated drug resistance of multiple myeloma cells by up-regulating CDC5L

Liu

Guo

Sha

et al. 2020

Aging

View full text Add to dashboard Cite

This study aimed to investigate whether annexin A7 (ANXA7) could promote the cell cycle, proliferation and cell adhesion-mediated drug resistance (CAM-DR) of multiple myeloma (MM) cells by up-regulating cell division cycle 5-like (CDC5L). As a result, ANXA7 expression was increased in the serum of MM patients and the expression of ANXA7 and CDC5L was also increased in MM cell lines. ANXA7 overexpression promoted the proliferation and cycle of U266 and RPMI8226 cells. The expression of proliferation cell nuclear antigen (PCNA), KI67, cyclin dependent kinase 1 (CDK1) and cyclinB1 in transfected cells was consistent with the changes of proliferation and cell cycle. In co-culture system of BMSC cells and MM cells, expression of CD44, ICAM1 and VCAM1 in MM cells was increased, which was further increased by ANXA7 overexpression. Bortezomib could increase the apoptosis of U266 and RPMI8226 cells. In co-culture system of BMSC cells and MM cells, the promotion effects of bortezomib on apoptosis of MM cells was decreased, which was further suppressed by ANXA7 overexpression. The above effects exerted by ANXA7 overexpression could be reversed by ANXA7 interference. Moreover, ANXA7 was proved to be combined with CDC5L. CDC5L interference could inhibit the promotion effects of ANXA7 overexpression on proliferation and cell cycle and inhibition effects of ANXA7 overexpression on apoptosis of MM cells treated with bortezomib in co-culture system. In conclusion, ANXA7 could promote the cell cycle, proliferation and CAM-DR of MM cells by upregulating CDC5L.

show abstract

Section: Discussionsupporting

confidence: 70%

ANXA7 promotes the cell cycle, proliferation and cell adhesion-mediated drug resistance of multiple myeloma cells by up-regulating CDC5L

Liu

Guo

Sha

et al. 2020

Aging

View full text Add to dashboard Cite

show abstract

“…The depletion of CDC5L leads to the inhibition of mitotic progression, and it induces mitotic failure [80]. Additionally, the downregulation of CDC5l inhibits the proliferation of bladder cancer cells [81]. ANLN has been experimentally proven to interact with CDC5L [82].…”

Section: Other Binding Partnersmentioning

confidence: 99%

Role of Anillin in Tumour: From a Prognostic Biomarker to a Novel Target

Tuan

Lee

2020

Cancers

View full text Add to dashboard Cite

Anillin (ANLN), an actin-binding protein, reportedly plays a vital role in cell proliferation and migration, particularly in cytokinesis. Although there have been findings pointing to a contribution of ANLN to the development of cancer, the association of ANLN to cancer remains not fully understood. Here, we gather evidence to determine the applicability of ANLN as a prognostic tool for some types of cancer, and the impact that ANLN has on the hallmarks of cancer. We searched academic repositories including PubMed and Google Scholar to find and review studies related to cancer and ANLN. The conclusion is that ANLN could be a potent target for cancer treatment, but the roles ANLN, other than in cytokinesis and its influence on tumour microenvironment remodeling in cancer development, must be further elucidated, and specific ANLN inhibitors should be found.

show abstract

“…A previous study found that Cdc5L is a regulator of mitotic progression as a pre-mRNA splicing factor. The deletion of endogenous Cdc5L decreases cell viability via dramatic mitotic arrest [ 29 ], which has not been reported in plants, but has been widely studied in animals, especially in the proliferation of cancer cells [ 30 ]. Cdc5L is also essential for porcine oocyte maturation [ 31 ].…”

Section: Resultsmentioning

confidence: 99%

Comparative proteomics analysis reveals differentially accumulated proteins associated with male and female A. chinensis var. chinensis bud development

et al. 2021

View full text Add to dashboard Cite

Background Kiwifruit (Actinidia chinensis var. Chinensis) is abundant with vitamin C and is a rapidly developing crop in China, New Zealand, and other countries. It has been widely used as a raw material for food and kiwifruit wine. Among these, A. chinensis var. chinensis and A. chinensis var. deliciosa are the most valuable kiwifruit in production. Kiwifruit is a typical dioecious plant and its female and male plants have different economic values. Therefore, sex identification, especially at the seedling stage, has important implications for the scientific planning of its production and economic benefits. However, the kiwifruit sex regulation mechanism is very complex and molecular studies are in the initial stages. Currently, there is not a universal and effective sex identification method for A. chinensis. Methods In this study, we used a label-free quantitative proteomics approach to investigate differentially accumulated proteins, including their presence/absence and significantly different levels of abundances during A. chinensis var. chinensis male and female flower bud development. Results A total of 6485 proteins were identified, among which, 203 were identified in male buds, which were mainly associated with phenylalanine metabolism, tyrosine metabolism, and plant hormone signal transduction. In female buds, 241 were identified, which were mainly associated with the ErbB signaling pathway, growth hormone synthesis, secretion and action, and mRNA surveillance pathway. A total of 373 proteins were significantly differentially accumulated proteins (fold change > 2; P < 0.05), of which, 168 were upregulated and 205 were downregulated. Significant differences between proteins involved 13 signaling pathways, most of which were involved in flavonoid biosynthesis, phenylpropanoid biosynthesis, and starch and sucrose metabolism. Protein interaction analysis showed that enriched protein nodes included cell division cycle 5-like protein, 40S ribosomal protein S8, ribosomal protein, and 40S ribosomal protein like, which interact with 35, 25, 22, and 22 proteins, respectively. Conclusions This study provide valuable information for cloning key genes that control sex traits and functionally analyze their roles, which lay a foundation to the development of molecular markers for male and female kiwifruit identification.

show abstract

Depletion of CDC5L inhibits bladder cancer tumorigenesis

Cited by 31 publications

References 24 publications

ANXA7 promotes the cell cycle, proliferation and cell adhesion-mediated drug resistance of multiple myeloma cells by up-regulating CDC5L

ANXA7 promotes the cell cycle, proliferation and cell adhesion-mediated drug resistance of multiple myeloma cells by up-regulating CDC5L

Role of Anillin in Tumour: From a Prognostic Biomarker to a Novel Target

Comparative proteomics analysis reveals differentially accumulated proteins associated with male and female A. chinensis var. chinensis bud development

Contact Info

Product

Resources

About