Dependence of fatty acid composition of Listeria spp. on growth temperature

Püttmann, M.; Ade, N.; H, Hof

doi:10.1016/0923-2508(93)90012-q

Cited by 49 publications

(30 citation statements)

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“…Phase transition temperatures of diacylphosphatidycholine with these fatty acids are as follows: Ϫ16.5°C for anteiso-C 15:0 , 7.6°C for anteiso-C 17:0 , 6.5°C for iso-C 14:0 , 22°C for iso-C 16:0 , 6.5°C for iso-C 15:0 , and 27.0°C for iso-C 17:0 (18). Anteiso-C 15:0 has a significantly greater ability to lower lipid phase transition temperature than other fatty acids, and it typically increases to constitute more than 60% of the total fatty acids when Listeria is grown at temperatures of 10°C or below (2,15,21,24,26). A similar high content of anteiso-C 15:0 (90%) was observed in a psychrotolerant Pseudomonas species when it was grown at 5°C (11).…”

supporting

confidence: 49%

“…The strategy for many gram-negative bacteria is primarily to increase the content of straight-chain unsaturated fatty acids (29). In contrast, the strategy for the gram-positive bacterium L. monocytogenes appears to be solely dependent upon increasing the content of the branched-chain anteiso-C 15:0 fatty acid (2,15,21,24,26). Bacillus subtilis, a close relative of L. monocytogenes, appears to regulate membrane fluidity by both unsaturated and branched-chain fatty acid (BCFA) strategies (9,19,31).…”

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confidence: 99%

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Exogenous Isoleucine and Fatty Acid Shortening Ensure the High Content of Anteiso-C _15:0 Fatty Acid Required for Low-Temperature Growth of Listeria monocytogenes

Zhu

Ding

Julotok

et al. 2005

Appl Environ Microbiol

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Previous studies have demonstrated that the branched-chain fatty acid anteiso-C 15:0 plays a critical role in the growth of Listeria monocytogenes at low temperatures by ensuring sufficient membrane fluidity. Studies utilizing a chemically defined minimal medium revealed that the anteiso fatty acid precursor isoleucine largely determined the fatty acid profile and fatty acid response of the organism to lowered growth temperature. When isoleucine was sufficient, the fatty acid profile was very uniform, with anteiso fatty acids comprising up to 95% of total fatty acid, and the major fatty acid adjustment to low temperature was fatty acid chain shortening, which resulted in an increase of anteiso-C 15:0 solely at the expense of anteiso-C 17:0 . When isoleucine was not supplied, the fatty acid profile became more complex and was readily modified by leucine, which resulted in a significant increase of corresponding iso fatty acids and an inability to grow at 10°C. Under this condition, the increase of anteiso-C 15:0 at low temperature resulted from the combined effect of increasing the anteiso:iso ratio and chain shortening. A branched-chain ␣-keto acid dehydrogenase-defective strain largely lost the ability to increase the anteiso:iso ratio. Cerulenin, an inhibitor of ␤-ketoacyl-acyl carrier protein synthase (FabF), induced a similar fatty acid chain shortening as low temperature did. We propose that the anteiso precursor preferences of enzymes in the branched-chain fatty acid biosynthesis pathway ensure a high production of anteiso fatty acids, and cold-regulated chain shortening results in a further increase of anteiso-C 15:0 at the expense of anteiso-C 17:0 .

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confidence: 49%

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confidence: 99%

Exogenous Isoleucine and Fatty Acid Shortening Ensure the High Content of Anteiso-C _15:0 Fatty Acid Required for Low-Temperature Growth of Listeria monocytogenes

Zhu

Ding

Julotok

et al. 2005

Appl Environ Microbiol

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“…Up to now, studies of BCFA have mainly focused on gram-positive bacteria because most BCFA-producing strains are gram positive. Previous studies have revealed that BCFA is necessary for the growth of gram-positive bacteria at low temperatures (9,41). In the regulation of BCFA synthesis, although ␤-ketoacyl-acyl carrier protein synthase III (FabH) was substantiated as a determining factor in BCFA synthesis based on its preference, there was no evidence that its preference in substrates was controlled by temperature or pressure.…”

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confidence: 77%

Role and Regulation of Fatty Acid Biosynthesis in the Response of Shewanella piezotolerans WP3 to Different Temperatures and Pressures

et al. 2009

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Members of the genus Shewanella inhabit various environments; they are capable of synthesizing various types of low-melting-point fatty acids, including monounsaturated fatty acids (MUFA) and branched-chain fatty acids (BCFA) with and without eicosapentanoic acid (EPA). The genes involved in fatty acid synthesis in 15 whole-genome-sequenced Shewanella strains were identified and compared. A typical type II fatty acid synthesis pathway in Shewanella was constructed. A complete EPA synthesis gene cluster was found in all of the Shewanella genomes, although only a few of them were found to produce EPA. The roles and regulation of fatty acids synthesis in Shewanella were further elucidated in the Shewanella piezotolerans WP3 response to different temperatures and pressures. The EPA and BCFA contents of WP3 significantly increased when it was grown at low temperature and/or under high pressure. EPA, but not MUFA, was determined to be crucial for its growth at low temperature and high pressure. A gene cluster for a branched-chain amino acid ABC transporter (LIV-I) was found to be upregulated at low temperature. Combined approaches, including mutagenesis and an isotopic-tracer method, revealed that the LIV-I transporter played an important role in the regulation of BCFA synthesis in WP3. The LIV-I transporter was identified only in the cold-adapted Shewanella species and was assumed to supply an important strategy for Shewanella cold adaptation. This is the first time the molecular mechanism of BCFA regulation in bacteria has been elucidated.

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“…In vitro MICs to Listeria monocytogenes are elevated compared to those to other Gram-positive bacteria, with an MIC 90 of 4 g/ml (64,65). The L. monocytogenes cell membrane is rich in branched fatty acids (66,67), which may prohibit optimal insertion and oligomerization of daptomycin in the cell membrane, but this has not been specifically demonstrated. Daptomycin does not display in vitro activity against the rapidly growing mycobacteria (68) or Rhodococcus or Nocardia species (64).…”

Section: Daptomycin Activity Against Gram-positive Bacteriamentioning

confidence: 99%

A Current Perspective on Daptomycin for the Clinical Microbiologist

2013

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SUMMARY Daptomycin is a lipopeptide antimicrobial with in vitro bactericidal activity against Gram-positive bacteria that was first approved for clinical use in 2004 in the United States. Since this time, significant data have emerged regarding the use of daptomycin for the treatment of serious infections, such as bacteremia and endocarditis, caused by Gram-positive pathogens. However, there are also increasing reports of daptomycin nonsusceptibility, in Staphylococcus aureus and, in particular, Enterococcus faecium and Enterococcus faecalis . Such nonsusceptibility is largely in the context of prolonged treatment courses and infections with high bacterial burdens, but it may occur in the absence of prior daptomycin exposure. Nonsusceptibility in both S. aureus and Enterococcus is mediated by adaptations to cell wall homeostasis and membrane phospholipid metabolism. This review summarizes the data on daptomycin, including daptomycin's unique mode of action and spectrum of activity and mechanisms for nonsusceptibility in key pathogens, including S. aureus , E. faecium , and E. faecalis . The challenges faced by the clinical laboratory in obtaining accurate susceptibility results and reporting daptomycin MICs are also discussed.

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Dependence of fatty acid composition of Listeria spp. on growth temperature

Cited by 49 publications

References 10 publications

Exogenous Isoleucine and Fatty Acid Shortening Ensure the High Content of Anteiso-C _15:0 Fatty Acid Required for Low-Temperature Growth of Listeria monocytogenes

Exogenous Isoleucine and Fatty Acid Shortening Ensure the High Content of Anteiso-C _15:0 Fatty Acid Required for Low-Temperature Growth of Listeria monocytogenes

Role and Regulation of Fatty Acid Biosynthesis in the Response of Shewanella piezotolerans WP3 to Different Temperatures and Pressures

A Current Perspective on Daptomycin for the Clinical Microbiologist

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Dependence of fatty acid composition of Listeria spp. on growth temperature

Cited by 49 publications

References 10 publications

Exogenous Isoleucine and Fatty Acid Shortening Ensure the High Content of Anteiso-C 15:0 Fatty Acid Required for Low-Temperature Growth of Listeria monocytogenes

Exogenous Isoleucine and Fatty Acid Shortening Ensure the High Content of Anteiso-C 15:0 Fatty Acid Required for Low-Temperature Growth of Listeria monocytogenes

Role and Regulation of Fatty Acid Biosynthesis in the Response of Shewanella piezotolerans WP3 to Different Temperatures and Pressures

A Current Perspective on Daptomycin for the Clinical Microbiologist

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Exogenous Isoleucine and Fatty Acid Shortening Ensure the High Content of Anteiso-C _15:0 Fatty Acid Required for Low-Temperature Growth of Listeria monocytogenes

Exogenous Isoleucine and Fatty Acid Shortening Ensure the High Content of Anteiso-C _15:0 Fatty Acid Required for Low-Temperature Growth of Listeria monocytogenes