Deoxyribonucleotide Biosynthesis: A Critical Process for Life

Follmann, Hartmut

doi:10.1007/978-94-009-7225-4_42

Cited by 4 publications

(2 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…FdUrd inhibits the enzyme thymidylate synthase, which catalyzes the reductive methylation of 2′-deoxyuridylate to form deoxythymidylate (Bachmann et al, 1983;Cisneros et al, 1993;Follmann, 1983).…”

Section: Inhibition Of Nuclear Dna Replicationmentioning

confidence: 99%

Accumulation of energy reserves in algae: From cell cycles to biotechnological applications

Vítová

Bišová

Kawano

et al. 2015

Biotechnology Advances

212

View full text Add to dashboard Cite

Starch and lipids are key components of algal cells and responsible for buffering variable supplies of energy and carbon that are vital for cell growth and reproduction, particularly DNA replication, nuclear and cellular division. The basic characteristics of energy reserves, their ultrastructure and localization inside the cell, regulation of their synthesis in relation to cell cycle phases, and their control by external factors, including light intensity, temperature, and carbon dioxide are described. Over the last two decades, research in this field has been boosted by possible biotechnological applications of algae for the production of biofuels from energy conserving compounds (bioethanol from starch and biodiesel from lipids). Recent findings on mechanisms that lead to an accumulation of exceptionally high levels of starch and lipids in algae will be summarized in this review. Macroelement (N, S, P) limitation, or depletion in mineral medium, as the most widely used approaches for enhancing both starch and lipid accumulation, are reviewed in detail. Potential biotechnological strategies for the economically viable overproduction of lipid and starch, such as a two-step procedure exploiting the effects of nutrient limitation and depletion, as well as the means and rationale for selecting appropriate strains, are discussed.

show abstract

Section: Inhibition Of Nuclear Dna Replicationmentioning

confidence: 99%

Accumulation of energy reserves in algae: From cell cycles to biotechnological applications

Vítová

Bišová

Kawano

et al. 2015

Biotechnology Advances

212

View full text Add to dashboard Cite

show abstract

“…The simplest experimental approach for solving this problem was to study the course of R N A accumulation using 5-fluorodeoxyuridine (FdUrd) to block DNA replication. FdUrd prevents DNA replication by inhibiting thymidylate synthase, the enzyme responsible for converting deoxyuridylate into deoxythymidylate (Follmann 1983). FdUrd effectively blocked DNA replication in Scenedesmus quadricauda cells, while growth processes were not affected by the presence of the inhibitor (Zachleder 1994).…”

Section: Key Index Words: Cell Cycle; Chlorophyta; Commitment To Divimentioning

confidence: 97%

REGULATION OF GROWTH PROCESSES DURING THE CELL CYCLE OF THE CHLOROCOCCAL ALGA SCENEDESMUS QUADRICAUDA UNDER A DNA REPLICATION BLOCK

Zachleder

1995

Journal of Phycology

View full text Add to dashboard Cite

The kinetics of two growth parameters (total RNA and total protein accumulation) was followed in synchronized cultures of the chlorococcal alga Scenedesmus quadricauda (Turp.) Bréb. under conditions of inhibited DNA replication in the presence of 5‐fluorodeoxyuridine (25 mg.L‐1). In the control culture, growth processes occurred in several steps with a decreasing rate of accumulation of RNA and protein amount approximately at each doubled value of the preceding step. Oscillations in the rate of growth processes in the control culture were temporally related to the initiation of individual reproductive steps. At each doubling, the cell became committed to triggering a sequence of reproductive processes, starting with DNA replication and ending with protoplast fission. Three commitment points were attained in the control culture and, consequently, three replication rounds of DNA followed by three nuclear divisions and three protoplast fissions occurred during one cell cycle. If 5‐fluorodeoxyuridine (FdUrd) was added at the beginning of the cell cycle, no reproductive processes occurred, and the cells remained uninuclear with one genome and did not divide. RNA accumulation did not seem to be affected by the presence of FdUrd for at least one cell cycle, and three or four doublings in the amount of RNA occurred during this period. Protein accumulation was even more independent of reproductive processes in the cell and continued for a period of about two or three cell cycles, attaining six doublings at the end of this period. Therefore, oscillations in the rate of protein or RNA accumulation remained even if reproductive processes were inhibited.

show abstract

Uncoupling of chloroplast reproductive events from cell cycle division processes by 5-fluorodeoxyuridine in the algaScenedesmus quadricauda

1996

View full text Add to dashboard Cite

Deoxyribonucleotide Biosynthesis: A Critical Process for Life

Cited by 4 publications

References 26 publications

Accumulation of energy reserves in algae: From cell cycles to biotechnological applications

Accumulation of energy reserves in algae: From cell cycles to biotechnological applications

REGULATION OF GROWTH PROCESSES DURING THE CELL CYCLE OF THE CHLOROCOCCAL ALGA SCENEDESMUS QUADRICAUDA UNDER A DNA REPLICATION BLOCK

Uncoupling of chloroplast reproductive events from cell cycle division processes by 5-fluorodeoxyuridine in the algaScenedesmus quadricauda

Contact Info

Product

Resources

About