Deoxycytidine production by metabolically engineered Corynebacterium ammoniagenes

Abstract

A number of essential oils from Mongolian aromatic plants are claimed to have antimicrobial activities. The essential oil of Dracocephalum foetidum, a popular essential oil used in Mongolian traditional medicine, was examined for its antimicrobial activity. Eight human pathogenic microorganisms including B. subtilis, S. aureus, M. lutens, E. hirae, S. mutans, E. coli, C. albicans, and S. cerevisiae were examined. The essential oil of Dracocephalum foetidum exhibited strong antimicrobial activity against most of the pathogenic bacteria and yeast strains that were tested; by both the agar diffusion method and the minimum inhibitory concentration (MIC) assay (MIC range was 26-2592 microg/ml). Interestingly, Dracocephalum foetidum even showed antimicrobial activity against methicillin-resistant Staphylococcus aureus (MRSA) strains. We also analyzed the chemical composition of the oil by GC-MS and identified several major components, including n-Mentha-1,8-dien-10-al, limonene, geranial, and neral.

Effect of Low Temperature Cultivation on the Phytochemical Profile and Bioactivity of Arctic Plants: A Case of Dracocephalum palmatum

“…The predominance of the acyclic monoterpenes perilla aldehyde and limonene was revealed in the EO from the aerial portions of D. surmondinum (54.3% and 30.1%, respectively) [ 30 ], D. multicaule (71.5% and 28.1%, respectively) [ 31 ] and D. polychaetum (63.4% and 22.1%, respectively) [ 32 ]. Other acyclic monoterpenes such as p -mentha-1,8-dien-10-al (39.2%), limonene (17.0%) and geranial (4.6%) prevailed in the EO from the aerial portion of D. foetidum [ 33 ]. The EO from the aerial portion of D. heterophyllum contained citronellol (74.9%), citronellyl formate (6.7%) and citronellal (6.7%) [ 34 ].…”

Antimicrobial Activity of Five Herbal Extracts Against Multi Drug Resistant (MDR) Strains of Bacteria and Fungus of Clinical Origin

“…Plants are rich in a wide variety of secondary metabolites such as tannins, alkaloids and flavonoids, which have been found in vitro to have antimicrobial properties [ 10 ]. A number of phytotherapy manuals have mentioned various medicinal plants for treating infectious diseases due to their availability, fewer side effects and reduced toxicity [ 11 ]. There are several reports on the antimicrobial activity of different herbal extracts [ 12 , 13 , 14 ].…”

The Family Corynebacteriaceae

“…Microbial degradation of squalene can provide optically active precursors for the industrial production of physiologically active compounds. Metabolically engineered strains of Corynebacterium ammoniagenes were shown to be useful for the overproduction of deoxycytidine (Lee et al 2011), uridine 5 0 -monophosphate (Wang et al 2007), and 5 0 -inosine monophosphate (Abbouni et al 2004). A large-scale production system of N-acetyllactosamine, a core structure of various oligosaccharides, was established by a whole-cell reaction through the combination of recombinant Escherichia coli strains and Corynebacterium ammoniagenes (Endo et al 1999).…”