Background: Dental student educational knowledge has an important role in the oral health education of their patients, families, and friends as well as their community by transmitting the correct oral habits and raising the awareness on how to prevent oral diseases. Aim: This study was conducted to evaluate the knowledge, attitude, and behavior toward the oral health of students studying dentistry in Cairo University, Egypt, using Hiroshima University-Dental Behavioral Inventory (HU-DBI) questionnaire. Methodology: The HU-DBI questionnaire was developed by Kawamura to evaluate oral health attitude, behavior, and self-reported knowledge. This questionnaire involves 20 items in a dichotomous response format (agree/disagree). As the English version of the questionnaire was used, and an Arabic version was adopted (mother tongue of the participants). It was distributed among 896 undergraduate students at the beginning or end of the practical section and was given a full explanation of the study. Each participant allowed 7-15 min to fill the questionnaire. The score of each item was analyzed and then a mean score was calculated. Results: There was a statistically significant difference in attitude and knowledge, while there was no statistically significant difference in behavior among dental student. The attitude and knowledge of clinical students are higher than that of preclinical, while behavior showed no statistically significant difference. Furthermore, oral health attitude of males was better than females, while oral health behavior of female dental students was better than males, as well as there was no statistically significant difference in knowledge between males and females in all levels. Conclusion: The oral health attitude and knowledge of clinical dental students are higher than that of preclinical students; however, oral health behavior was the same for all levels. Clinical Significant: The improvement of the dental student behavior and knowledge has a direct effect on the oral health of their patients, families, and their community that will lead to improvement of the general health.