Dental Stem Cell Migration on Pulp Ceiling Cavities Filled with MTA, Dentin Chips, or Bio-Oss

Abstract

MTA, Bio-Oss, and dentin chips have been successfully used in endodontics. The aim of this study was to assess the adhesion and migration of dental stem cells on human pulp ceiling cavities filled with these endodontic materials in an experimental model, which mimics the clinical conditions of regenerative endodontics. Cavities were formed, by a homemade mold, on untouched third molars, filled with endodontic materials, and observed with electron microscopy. Cells were seeded on cavities' surface and their morphology and number were analysed. The phenomenon of tropism was assessed in a migration assay. All three materials demonstrated appropriate microstructures for cell attachment. Cells grew on all reagents, but they showed a differential morphology. Moreover, variations were observed when comparing cells numbers on cavity's filling versus the surrounding dentine disc. The highest number of cells was recorded on dentin chips whereas the opposite was true for Bio-Oss. This was confirmed in the migration assay where a statistically significant lower number of cells migrated towards Bio-Oss as compared to MTA and dentin chips. This study highlights that MTA and dentin chips have a greater potential compared to Bio-Oss regarding the attraction of dental stem cells and are good candidates for bioengineered pulp regeneration.

Effect of an Experimental Direct Pulp-capping Material on the Properties and Osteogenic Differentiation of Human Dental Pulp Stem Cells

“…As shown in Figs 3 and 4 , Exp promoted cellular migration and adhesion. Our findings are in agreement with previous studies showing that MTA could accelerate the migration and adhesion of dental pulp-derived cells 35 36 . A migration assay also provided the first in vitro evidence of a similar migration effect of PC on hDPSCs, but Vincenzo et al 37 found that MTA enhances HMSC migration significantly more than PC.…”

Biocompatibility of three new calcium silicate‐based endodontic sealers on human periodontal ligament stem cells

“…The results of this study showed that extracts from Bioroot BC Sealer, Endoseal MTA and Nano-ceramic Sealer had dose-and time-dependent effects on the biological responses of hPDLSCs. Due to the excellent properties of mineral trioxide aggregate (MTA), new endodontic materials based on MTA have emerged recently (Bin et al 2012, Butt et al 2014, Zhu et al 2014, Lymperi et al 2015, Utneja et al 2015. However, the different compositions of MTA sealers are often unknown, and sealers have provided differing results for their biocompatibility in vitro and in vivo (G€ uven et al 2013(G€ uven et al , Chang et al 2014.…”

Differences in the cytocompatibility of bone-plastic materials from xenogeneic hydroxyapatite with stem cells from human exfoliated deciduous teeth and adipose tissue-derived mesenchymal stem cells

“…Данные о цитотоксическом влиянии на SHED костно-пластических материалов, содержащих ксеногенный ГА, соотносятся с результатами других исследований последних лет. Так, было показано, что миграция ММСК пульпарного происхождения в материал Bio-Oss происходит значительно менее интенсивно по сравнению с МТА-цементом и гранулированным дентином [13].…”