Dental pulp stem cells express proteins involved in the local invasiveness of odontogenic myxoma

Abstract

Little is known about the histogenesis of the odontogenic myxoma (OM). Dental pulp stem cells could be candidate precursors of OM because both OM and the dental pulp share the same embryological origin: the dental papilla. For the purpose of comparing OM and stem cells, this study analyzed the expression of two proteins related to OM invasiveness (MMP-2 and hyaluronic acid) in human immature dental pulp stem cells (hIDPSCs). Three lineages of hIDPSCs from deciduous and permanent teeth were used in this study. Immunofluorescence revealed positive reactions for MMP-2 and hyaluronic acid (HA) in all hIDPSCs. MMP-2 appeared as dots throughout the cytoplasm, whereas HA appeared either as diffuse and irregular dots or as short fibrils throughout the cytoplasm and outside the cell bodies. The gene expression profile of each cell lineage was evaluated using RT-PCR analysis, and HA was expressed more intensively than MMP-2. HA expression was similar among the three hIDPSCs lineages, whereas MMP-2 expression was higher in DL-1 than in the other cell lines. The expression of proteins related to OM invasiveness in hIDPSCs could indicate that OM originates from dental pulp stem cells.

Odontogenic Myxoma: Systematic review and bias analysis

“…Inactivating mutations of PRKAR1A gene were also found in patients diagnosed with OM, and this gene regulates a subunit type 1A of cAMP‐dependent PKA 62 . Interestingly, some previous studies suggested that dental pulp stem cells may be the precursors of OM, but more studies should be performed 65,66 …”

“…62 Interestingly, some previous studies suggested that dental pulp stem cells may be the precursors of OM, but more studies should be performed. 65,66 Maybe, the frequency difference between study populations with higher prevalence in Asian and American population may be explained by genetic susceptibility and polymorphism of some gene groups, as happens in odontogenic keratocystic tumour 67 and ameloblastoma; 68 however, no study evaluated this characteristic for OM.…”

Oncologic profile of maxillary odontogenic myxoma: A rare case

“…One of them is related to the histogenesis of the odontogenic neoplasms. [7] Majority of tumors occur in jaw bearing areas therefore the word odontogenic is prefixed to reflect its odontogenic origin. The resemblance to mesenchyme of developing tooth or the periodontal ligament suggests its histogenesis.…”

“…A high degree of matrix metalloproteinases 2 (MMP2) expression was found in cell lines derived from OM tumor. [7]…”

Análise do efeito de substâncias liberadas por adesivos dentinários sobre a atividade e a expressão gênica de proteases da matriz extracelular (MMPs e CTs) em células-tronco da polpa dentária humana

“…Em situações patológicas da cavidade bucal, as MMPs têm sido relacionadas a tipos de neoplasias (Miyagi et al, 2012), doença periodontal (Sorsa et al, 2004), inflamação pulpar (Hannas et al, 2007) e cárie (Tjäderhane et al, 1998). Nesse último exemplo, ainda na década de 80 e sem indicar especificamente as MMPs, Dayan et al (1983) realizaram um estudo preliminar comparando a atividade colagenolítica em dentina humana cariada e não-cariada e encontraram maior atividade enzimática em dentina cariada, sugerindo que as enzimas responsáveis pela degradação de colágeno seriam ativadas durante o processo de cárie dental.…”