Dental follicle cell-conditioned medium enhances the formation of osteoclast-like multinucleated cells

Kawakami, Masayoshi; Kuroda, Shingo; Yoshida, Chisato; Yamashita, Kazuhito; Takada, Kenji

doi:10.1093/ejo/22.6.675

Cited by 3 publications

(2 citation statements)

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“…The underlying tooth bud contributes to primary tooth root resorption either through direct pressure (Sahara, 2001), or via genetically programmed signals sent from the dental follicle independent of pressure. This is evidenced by the findings that conditioned media from dental follicles promotes osteoclastogenesis and the tightly controlled temporal expression of receptor activator for nuclear factor κB ligand (RANKL) in the dental follicle (Kawakami et al , 2000; Liu et al , 2005).…”

Section: Introduction and Clinical Scenariosmentioning

confidence: 99%

Osteoclasts and odontoclasts: signaling pathways to development and disease

Wang¹,

McCauley

2011

Oral Diseases

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Oral Diseases (2011) 17, 129–142 Osteoclasts are cells essential for physiologic remodeling of bone and also play important physiologic and pathologic roles in the dentofacial complex. Osteoclasts and odontoclasts are necessary for tooth eruption yet result in dental compromise when associated with permanent tooth internal or external resorption. The determinants that separate their physiologic and pathologic roles are not well delineated. Clinical cases of primary eruption failure and root resorption are challenging to treat. Mineralized tissue resorbing cells undergo a fairly well characterized series of differentiation stages driven by transcriptional mediators. Signal transduction via cytokines and integrin‐mediated events comprise the detailed pathways operative in osteo/odontoclastic cells and may provide insights to their targeted regulation. A better understanding of the unique aspects of osteoclastogenesis and osteo/odontoclast function will facilitate effective development of new therapeutic approaches. This review presents the clinical challenges and delves into the cellular and biochemical aspects of the unique cells responsible for resorption of mineralized tissues of the craniofacial complex.

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Section: Introduction and Clinical Scenariosmentioning

confidence: 99%

Osteoclasts and odontoclasts: signaling pathways to development and disease

Wang¹,

McCauley

2011

Oral Diseases

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“…DFCs secretomes was considered to be therapeutic potential. Conditioned medium (CM) from DFCs culture could induce osteogenic and cementogenic differentiation in vitro 11 , 12 and the formation of dental pulp-dentin complex in vivo 13 .…”

Section: Introductionmentioning

confidence: 99%

Secretome profiles of immortalized dental follicle cells using iTRAQ-based proteomic analysis

Dou

Yan

et al. 2017

Sci Rep

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Secretomes produced by mesenchymal stromal cells (MSCs) were considered to be therapeutic potential. However, harvesting enough primary MSCs from tissue was time-consuming and costly, which impeded the application of MSCs secretomes. This study was to immortalize MSCs and compare the secretomes profile of immortalized and original MSCs. Human dental follicle cells (DFCs) were isolated and immortalized using pMPH86. The secretome profile of immortalized DFCs (iDFCs) was investigated and compared using iTRAQ labeling combined with mass spectrometry (MS) quantitative proteomics. The MS data was analyzed using ProteinPilotTM software, and then bioinformatic analysis of identified proteins was done. A total of 2092 secreted proteins were detected in conditioned media of iDFCs. Compared with primary DFCs, 253 differently expressed proteins were found in iDFCs secretome (142 up-regulated and 111 down-regulated). Intensive bioinformatic analysis revealed that the majority of secreted proteins were involved in cellular process, metabolic process, biological regulation, cellular component organization or biogenesis, immune system process, developmental process, response to stimulus and signaling. Proteomic profile of cell secretome wasn’t largely affected after immortalization converted by this piggyBac immortalization system. The secretome of iDFCs may be a good candidate of primary DFCs for regenerative medicine.

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