Density of Demodex folliculorum in Perioral Dermatitis

Dolenc‐Voljč, Mateja; Pohar, Maja; Lunder, Tomaž

doi:10.1080/00015550510030069

Cited by 67 publications

(49 citation statements)

References 32 publications

(39 reference statements)

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“…That finding was confirmed by the present study (OR=8.1). The hypothesis that SID and Seb D were associated with Demodex mites has not been confirmed at present, but our results were consistent with those of Dolenc-Voljc et al (2005) and Karincaoglu et al (2008;.…”

Section: Discussionsupporting

confidence: 82%

“…Demodex mites are usually considered to play a pathogenic role when present in an excessive number or penetrating into the dermis (Ayres and Ayres, 1961;Ecker and Winkelmann, 1979;Bonnar et al, 1993;Forton and Seys, 1993;Erbagci and Ozgoztasi, 1998). They have been implicated in the occurrence of a wide range of clinical features, including pityriasis folliculorum (Ayres and Ayres, 1961), papulopustular and granulomatous rosacea (Bonnar et al, 1993;Forton and Seys, 1993), inflammatory papule (Seifert, 1978), folliculitis (Purcell et al, 1986), seborrheic dermatitis (Karincaoglu et al, 2009), perioral dermatitis (Dolenc-Voljc et al, 2005), and blepharitis (Post and Juhlin, 1963;Zhao et al, 2011a), although much controversy persists (Bonnar et al, 1993;Forton and Seys, 1993;Forton et al, 2005;Hsu et al, 2009). A definitive diagnosis of demodicosis requires a compatible clinical picture and the presence of more than 5 mites/cm 2 (Forton and Seys, 1993).…”

Section: Introductionmentioning

confidence: 99%

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Facial dermatosis associated with Demodex: a case-control study

Zhao

Yan

Wang

et al. 2011

J. Zhejiang Univ. Sci. B

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Abstract:Demodex has been considered to be related with multiple skin disorders, but controversy persists. In this case-control study, a survey was conducted with 860 dermatosis patients aged 12 to 84 years in Xi'an, China to identify the association between facial dermatosis and Demodex. Amongst the patients, 539 suffered from facial dermatosis and 321 suffered from non-facial dermatosis. Demodex mites were sampled and examined using the skin pressurization method. Multivariate regression analysis was applied to analyze the association between facial dermatosis and Demodex infestation, and to identify the risk factors of Demodex infestation. The results showed that total detection rate of Demodex was 43.0%. Patients aged above 30 years had higher odds of Demodex infestation than those under 30 years. Compared to patients with neutral skin, patients with mixed, oily, or dry skin were more likely to be infested with Demodex (odds ratios (ORs) were 2.5, 2.4, and 1.6, respectively). Moreover, Demodex infestation was found to be statistically associated with rosacea (OR=8.1), steroid-induced dermatitis (OR=2.7), seborrheic dermatitis (OR=2.2), and primary irritation dermatitis (OR=2.1). In particular, ORs calculated from the severe infestation (≥5 mites/cm 2 ) rate were significantly higher than those of the total rate. Therefore, we concluded that Demodex is associated with rosacea, steroid-induced dermatitis, seborrheic dermatitis, and primary irritation dermatitis. The rate of severe infestation is found to be more correlated with various dermatosis than the total infestation rate. The risk factors of Demodex infestation, age, and skin types were identified. Our study also suggested that good hygiene practice might reduce the chances of demodicosis and Demodex infestation.

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Section: Discussionsupporting

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Facial dermatosis associated with Demodex: a case-control study

Zhao

Yan

Wang

et al. 2011

J. Zhejiang Univ. Sci. B

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“…A small number of Demodex mites seem to be harmless residents of the skin; however, the presence of larger-than-normal numbers of Demodex mites has been suggested to be pathogenic. D. folliculorum has been incriminated as playing a role in the development of clinical dermatological conditions such as rosacea, granulomatus perioral dermatitis, blepharitis, pityriasis folliculorum and pustular folliculitis, although much controversy persists (English and Nutting 1981;Gutgesell et al 1982;Clifford and Fulk 1990;Corredor-Osorio et al 2000;Dolenc-Volje et al 2005;Elston 2005Elston , 2010Kligman and Christensen 2011;Lacey et al 2011).…”

Section: Introductionmentioning

confidence: 97%

Morphobiometrical and molecular study of two populations of Demodex folliculorum from humans

et al. 2011

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A morphobiometrical and molecular study of two populations of Demodex folliculorum from humans isolated from different habitats, skin and eyelashes follicles, were carried out. Morphological and biometrical studies revealed two closely related populations with any distinctive characteristics. For molecular study, a 436-bp region of the 16S rDNA gene and a 453-bp region of the cytochrome oxidase I (COI) gene from individual mites of each population considered were sequenced. Intraindividual and interindividual sequence variation was studied in both populations. Our data show that 16S rDNA is not a useful marker to discriminate between populations; however, COI gene sequences can help to identify the two populations considered, which are morphologically very close and difficult to separate by classic methods. These results are in agreement with the morphological and biometrical differences detected between D. folliculorum from eyelashes and human skin. This study appeals for the revision of the taxonomic status of the D. folliculorum populations, as well as for the species included within genus Demodex.

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“…It has been reported that the pathogenicity of Demodex is related to population. In humans, the association between D. folliculorum and pityriasis folliculorum, rosacea, perioral dermatitis, and blepharitis is stronger than that between D. brevis and those diseases (Ayres and Ayres 1961;Forton and Seys 1993;Dolenc-Voljc et al 2005). However, in dog, whether Demodex injai or D. canis is pathogenetic remains controversial (Hillier and Desch 2002;Sastre et al 2013).…”

Section: Discussionmentioning

confidence: 95%

Molecular identification and phylogenetic study of Demodex caprae

Zhao

Juan

et al. 2014

Parasitol Res

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The DNA barcode has been widely used in species identification and phylogenetic analysis since 2003, but there have been no reports in Demodex. In this study, to obtain an appropriate DNA barcode for Demodex, molecular identification of Demodex caprae based on mitochondrial cox1 was conducted. Firstly, individual adults and eggs of D. caprae were obtained for genomic DNA (gDNA) extraction; Secondly, mitochondrial cox1 fragment was amplified, cloned, and sequenced; Thirdly, cox1 fragments of D. caprae were aligned with those of other Demodex retrieved from GenBank; Finally, the intra- and inter-specific divergences were computed and the phylogenetic trees were reconstructed to analyze phylogenetic relationship in Demodex. Results obtained from seven 429-bp fragments of D. caprae showed that sequence identities were above 99.1% among three adults and four eggs. The intraspecific divergences in D. caprae, Demodex folliculorum, Demodex brevis, and Demodex canis were 0.0-0.9, 0.5-0.9, 0.0-0.2, and 0.0-0.5%, respectively, while the interspecific divergences between D. caprae and D. folliculorum, D. canis, and D. brevis were 20.3-20.9, 21.8-23.0, and 25.0-25.3, respectively. The interspecific divergences were 10 times higher than intraspecific ones, indicating considerable barcoding gap. Furthermore, the phylogenetic trees showed that four Demodex species gathered separately, representing independent species; and Demodex folliculorum gathered with canine Demodex, D. caprae, and D. brevis in sequence. In conclusion, the selected 429-bp mitochondrial cox1 gene is an appropriate DNA barcode for molecular classification, identification, and phylogenetic analysis of Demodex. D. caprae is an independent species and D. folliculorum is closer to D. canis than to D. caprae or D. brevis.

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Density of Demodex folliculorum in Perioral Dermatitis

Cited by 67 publications

References 32 publications

Facial dermatosis associated with Demodex: a case-control study

Facial dermatosis associated with Demodex: a case-control study

Morphobiometrical and molecular study of two populations of Demodex folliculorum from humans

Molecular identification and phylogenetic study of Demodex caprae

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