Density-Gradient centrifugation of DNA in a fixed-angle rotor

Linear Association Between Cellular DNA and Epstein-Barr Virus DNA in a Human Lymphoblastoid Cell Line

“…CsCl gradient centrifugation of DNA in fixed-angle head rotors gives better resolution and loading capacity than centrifugation in conventional swinging-bucket rotors (12). However, when DNA of molecular weight 1 to 2 X 108 is fractionated, the capacity of even such gradients is markedly reduced.…”

“…Here we describe experiments performed in neutral solution that indicate that there is a linear association between EBV DNA and cellular DNA in transformed human lymphocytes. (12,13).…”

In vitro methylation of total and foldback DNAs in normal and virus‐transformed cells

“…Unlike this fraction, which contained inverted repeats of the type ABC.. . CBA (C/CO = e&"), sedimentation in a CsCl gradient at neutral pH (9)(10)(11)(12)(13) suggested that the fraction which did not reassociate in the second step represented ordinary repetitive sequences, including satellites, of the type ABC.. . ABC.…”

Hybridization of prokaryotic and eukaryotic 5S rRNA to Euglena gracilis chloroplast DNA

“…Chloroplast DNA of mean density 1.685 g/cm 3 was collected after preparative CsC1 density gradient centrifugation [24] neglecting the DNA shoulder of density 1.700 g/cm 3, although this is known to result in the preferential loss of some rRNA cistrons [25]. The purity of the chloroplast DNA was measured by analytical CsC1 density gradient centrifugation against a Micrococcus luteus DNA standard, of density 1.731 g/cm a.…”