Linear Association Between Cellular DNA and Epstein-Barr Virus DNA in a Human Lymphoblastoid Cell Line

Adams, Alice; Lindahl, Tomas; Klein, George

doi:10.1073/pnas.70.10.2888

Cited by 94 publications

(43 citation statements)

References 20 publications

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“…EBV infects two main cell types, B lymphocytes and epithelial cells. The first reports on the possible integration of the EBV genome into human chromosomes date back to the mid-to late 1970s (3,4,50,54,89,104). At that time, the conclusion that EBV was integrated was derived mostly from isopycnic centrifugation experiments.…”

Section: Epstein-barr Virusmentioning

confidence: 99%

Herpesviruses and Chromosomal Integration

Morissette

Flamand

2010

J Virol

200

162

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Herpesviruses are members of a diverse family of viruses that colonize all vertebrates from fish to mammals. Although more than one hundred herpesviruses exist, all are nearly identical architecturally, with a genome consisting of a linear double-stranded DNA molecule (100 to 225 kbp) protected by an icosahedral capsid made up of 162 hollow-centered capsomeres, a tegument surrounding the nucleocapsid, and a viral envelope derived from host membranes. Upon infection, the linear viral DNA is delivered to the nucleus, where it circularizes to form the viral episome. Depending on several factors, the viral cycle can proceed either to a productive infection or to a state of latency. In either case, the viral genetic information is maintained as extrachromosomal circular DNA. Interestingly, however, certain oncogenic herpesviruses such as Marek's disease virus and Epstein-Barr virus can be found integrated at low frequencies in the host's chromosomes. These findings have mostly been viewed as anecdotal and considered exceptions rather than properties of herpesviruses. In recent years, the consistent and rather frequent detection (in approximately 1% of the human population) of human herpesvirus 6 (HHV-6) viral DNA integrated into human chromosomes has spurred renewed interest in our understanding of how these viruses infect, replicate, and propagate themselves. In this review, we provide a historical perspective on chromosomal integration by herpesviruses and present the current state of knowledge on integration by HHV-6 with the possible clinical implications associated with viral integration.

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Section: Epstein-barr Virusmentioning

confidence: 99%

Herpesviruses and Chromosomal Integration

Morissette

Flamand

2010

J Virol

200

162

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“…Human lymphoblastoid cell lines transformed by Epstein-Barr virus (EBV) are already shown to contain variable copies of the virus genome (Nonoyama & Pagano, 197I ;Zur Hausen et al 1972). Furthermore, in one human lymphoblastoid line, a linear association is shown, between EBV DNA and host cell DNA (Adams, Lindahl & Klein, 1973).…”

Section: Deoxyribonucleic Acid Of Marek's Disease Virus In a Lymphoblmentioning

confidence: 99%

Deoxyribonucleic Acid of Marek's Disease Virus in a Lymphoblastoid Cell Line from Marek's Disease Tumours

Nazerian¹,

Lee²

1974

Journal of General Virology

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“…Raji cells contain [50][51][52][53][54][55][56][57][58][59][60] EBV genome equivalents per cell in latent form (6). We have previously shown that DNA "joint molecules" comprised of both cellular and viral DNA sequences can be isolated from Raji cells (7), and that at least part of this association between viral DNA and cellular DNA is due to alkali-stable bonds (8). These 15 volumes of deaerated 50 mM Tris HCl (pH 8.5).…”

mentioning

confidence: 99%

“…The preparation of EBV ["2P] cRNA and the hybridization procedures have been described (7,11). To each gradient fraction containing DNA to be analyzed, one volume of 1 AM NaOH and 0-0.005 volume of 0.1% salmon sperm DNA were added, to obtain an alkalidenatured DNA solution containing a total of 5 /g of DNA.…”

mentioning

confidence: 99%

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Epstein-Barr virus genomes with properties of circular DNA molecules in carrier cells.

Adams¹,

Lindahl²

1975

Proc. Natl. Acad. Sci. U.S.A.

144

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A high-density fraction of high-molecular- After an additional 40 hr of incubation, the cells were harvested and washed twice with sodium phosphate-buffered saline. The cells were then suspended at a concentration of 107 cells per ml in phosphate-buffered saline and lysed by the addition of 0.5 volume of 3% Sarkosyl (Geigy) in 75 mM Tris HCl/25 mM EDTA (pH 9.0). After gentle rolling to obtain a clear, viscous lysate, 0.1 volume of 1% Pronase was added, and the mixture was incubated for 2 hr at 37°. The solution was then cooled to room temperature (210) and diluted with 15 volumes of deaerated 50 mM Tris HCl (pH 8.5). After 1 hr of slow swirling to obtain a homogeneous solution containing 5,/g/ml of DNA, solid CsCl was added and dissolved in the viscous DNA solution. In some experiments, the DNA solution was further treated with phenol as described (7), followed by dialysis, prior to addition of the CsCl.EBV [3H]DNA (7 X 104 cpm/ug) was isolated from virus particles recovered from the P3HR-1 cell line as described (7).[

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Linear Association Between Cellular DNA and Epstein-Barr Virus DNA in a Human Lymphoblastoid Cell Line

Abstract: High-molecular-weight DNA from cell line Raji

Cited by 94 publications

References 20 publications

Herpesviruses and Chromosomal Integration

Herpesviruses and Chromosomal Integration

Deoxyribonucleic Acid of Marek's Disease Virus in a Lymphoblastoid Cell Line from Marek's Disease Tumours

Epstein-Barr virus genomes with properties of circular DNA molecules in carrier cells.

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