Density and diameter of dentinal tubules in etched and non-etched bovine dentine examined by scanning electron microscopy

Dutra-Correa, Maristela; Anauate-Netto, Camillo; Arana‐Chavez, Victor E.

doi:10.1016/j.archoralbio.2007.03.003

Cited by 30 publications

(36 citation statements)

References 16 publications

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“…Reis et al (1), showed by scanning electron microscopy that human and bovine coronal dentin substrates are very much alike with regard to number and diameter of tubules in mid-depth dentin, and the comparison of enamel morphology after acid etching revealed that the human and bovine enamel showed apparently similar aspects (1). Although these studies show similarity in morphology, other studies (5,14) have demonstrated that bovine dentin has some different structural aspects from those reported for human dentin, mainly related to the diameter of dentinal tubules and to the thickness of peritubular dentin at the several depths (14). However, as the fracture strength is a macroscopic test, small differences between the structures do not seem to produce significant influence on the final strength.…”

Section: Discussionmentioning

confidence: 78%

“…As far as the dental structure is concerned, despite some structural differences between bovine and human teeth (5,14), several studies (1,4) consider bovine teeth as possible substitutes for human teeth in dentin or enamel bond testing. Histochemical and comparative morphological studies have revealed that all mammalian teeth are essentially similar (13).…”

Section: Discussionmentioning

confidence: 99%

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Fracture strength of composite fixed partial denture using bovine teeth as a substitute for human teeth with or without fiber-reinforcement

et al. 2010

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This study evaluate the use of bovine teeth as a substitute for human teeth on fracture strength tests of composite fixed partial dentures (Cpd), with and without fiberglass reinforcement (Fg). Eighty teeth were selected, being 40 bovine incisors, 20 human premolars and 20 molars. Bovine incisors were ground to get a platform, simulating an occlusal surface of human molar. Teeth in pairs were embedded in polystyrene resin, simulating the periodontal ligament and divided in 4 groups: B-Cpd-Fg: bovine teeth restored with Cpd with Fg; B-Cpd-NFg: bovine teeth restored with Cpd without Fg; H-Cpd-Fg: human teeth restored Cpd with Fg; and H-Cpd-NFg: human teeth restored with Cpd without Fg. The Cpd were adhesively fixed and submitted to an axial compression load at the pontic center with a crosshead speed of 0.5 mm/min until fracture. Failure modes were assessed and classified. Data were subjected to two-way ANOVA and Tukey's HSD test (α=0.05). The tooth type had no influence on fracture strength and fracture mode. The inclusion of fiberglass increased significantly the fracture strength. The failure modes were more reparable in groups with fiber-reinforcement. Bovine teeth can be used as a substitute for human teeth in these types of fracture strength tests.

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Section: Discussionmentioning

confidence: 78%

Section: Discussionmentioning

confidence: 99%

Fracture strength of composite fixed partial denture using bovine teeth as a substitute for human teeth with or without fiber-reinforcement

et al. 2010

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“…Due to lower density of DT [7], DT diameter declining in pulpal direction [7,8] and thicker PTD [7] the use of bovine dentin as a model for dental research is questioned. On the other hand, the great similarity in microstructural and chemical composition of porcine and human dentin points to suitability of porcine dentin use in human dental research.…”

Section: Discussionmentioning

confidence: 99%

“…In human tooth DT diameters are approximately 0.9 µm at the DEJ and 2.5 µm at the surface facing a dental pulp where DT are also packed closer together [4]. Similarly, diameters of DT decrease towards the external layers of dentin in some animals (in canine teeth from 2.1 µm to 0.9 µm [5,6] and in equine teeth from 2.5 µm to 1.0 µm) [3], while in bovine teeth DT diameters are narrower at the dentin surface facing a pulp comparing to those at the periphery [7,8] (with main DT diameters measuring 1.8 µm at a pulp side and 2.3 µm at DEJ) [7].…”

Section: Introductionmentioning

confidence: 95%

“…In studies focusing adhesion to enamel and dentine bovine incisors are commonly employed as a substitute to human teeth [28,29], since they are larger and are easily obtained. Nevertheless, the use of bovine dentin in such studies is being questioned due to different microstructural characteristics [7,8] which significantly affect the strength of the adhesive bonds created among restorative materials and dentin [30]. The aim of the study was, first of all to determine variations in microstructure of porcine, canine, equine and human dentin as analysed by scanning electron microscope (SEM).…”

Section: Introductionmentioning

confidence: 99%

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Animal and human dentin microstructure and elemental composition

et al. 2014

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AbstractAnimal teeth are a common model in studies on dentin adhesive materials. The aim of this study was to compare microstructural parameters (density and diameter of dentinal tubules (DT), peritubular dentin (PTD) thickness, PTD and intertubular dentin (ITD) surface area) and chemical characteristics of canine, porcine, equine, and human root dentin. The middle layers of dentin were harvested just below a cemento-enamel junction from incisors and investigated by means of scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDXS). SEM evaluation of the specimens revealed, that porcine dentin shared most similarities with human dentin. When comparing the density of DTs, canine dentin was also found to be similar to human dentin. Elemental composition of the root dentin did not differ significantly in porcine, equine and human dentin, but in canine dentin higher magnesium value in PTD compared to ITD was found. It is known that microstructural and chemical characteristics affect the strength of the adhesive bonds created among restorative materials and dentin. According to the results of this study, porcine dentin seems to be the most appropriate model to study dental materials to be used in human restorative dentistry.

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Elemental and chemical compounds study of enamel and dentine of porcine primary molar teeth

et al. 2019

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For the enamel and dentin of porcine primary molar teeth, the spatial variability of the elements and chemical groups was studied with the aim of identifying the chemistry of porcine teeth, and it was then compared with results for human primary teeth. Porcine teeth were taken from specially bred animals.An electron microprobe was applied for the elemental analyses and a Raman microscope for the determination of chemical groups. The distribution of the main elements (Ca, P) was similar for both porcine and human teeth, whereas major differences were found in the distributions of minor elements (Mg, Sr, Cl, C). Carbonates were present in porcine dentin mainly as A-substituted species, whereas the B-substituted ones prevailed in enamel. The mechanism of asymmetric enrichment of Ca and P was quantitatively calculated as a consequence of Mg release. The layer close to the surface of the enamel with air had a unique composition. Cl involvement in enamel close to the boundary with air is a complex process, coupled with the involvement of Sr and release of Mg. The discoveries shed light on the role of elements in apatites, especially the role of carbonates and magnesium in porcine teeth, and put in doubt the potential use of pig apatite as a substitute for human apatite in filling materials.

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Density and diameter of dentinal tubules in etched and non-etched bovine dentine examined by scanning electron microscopy

Cited by 30 publications

References 16 publications

Fracture strength of composite fixed partial denture using bovine teeth as a substitute for human teeth with or without fiber-reinforcement

Fracture strength of composite fixed partial denture using bovine teeth as a substitute for human teeth with or without fiber-reinforcement

Animal and human dentin microstructure and elemental composition

Elemental and chemical compounds study of enamel and dentine of porcine primary molar teeth

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