Densitometric Validation and Optimisation of Polyphenols in <i>Ocimum sanctum</i> Linn by High Performance Thin‐layer Chromatography

Ilyas, UK; Katare, Deepshikha Pande; Aeri, Vidhu

doi:10.1002/pca.2550

Cited by 7 publications

(2 citation statements)

References 40 publications

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“…The model in this study was significant ( p -value = 0.082). The achieved results were in agreement with previous published data on Ocimum sanctum where an HPTLC method was developed involving a Box–Behnken-supported design for the simultaneous optimization, validation, and quantification of polyphenols in an aqueous alcoholic extract [ 35 ].…”

Section: Resultssupporting

confidence: 89%

Optimization of Ultrasonicated Kaempferol Extraction from Ocimum basilicum Using a Box–Behnken Design and Its Densitometric Validation

Altemimi

Mohammed

Lee

et al. 2020

Foods

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Kaempferol (KA) is a natural flavonol that can be found in plants and plant-derived foods with a plethora of different pharmacological properties. In the current study, we developed an efficient extraction method for the isolation of KA from ultrasonicated basil leaves (Ocimum basilicum). We successfully employed a Box–Behnken design (BBD) in order to investigate the effect of different extraction variables including methanol concentration (40–80%), extraction temperature (40–60 °C), and extraction time (5–15 min). The quantification of KA yield was carried out by employing a validated densitometric high performance thin layer chromatography in connection with ultraviolet detection (HPTLC-VIS). The obtained data showed that the quadratic polynomial model (R2 = 0.98) was the most appropriate. The optimized ultrasonic extraction yielded 94.7 ng/spot of KA when using methanol (79.99%) at 60 °C for 5 min. When using toluene-ethyl acetate-formic acid (70:30:1 v/v/v) as a solvent, KA was detected in basil leaves at an Retention factor (Rf) value of 0.26 at 330 nm. Notably, the analytical method was successfully validated with a linear regression of R2 = 0.99, which reflected a good linear relationship. The developed HPTLC-VIS method in this study was precise, accurate, and robust due to the lower obtained results from both the percent relative standard deviation (%RSD) and SEM of the O. basilicum. The antioxidant activity of KA (half maximal inhibitory concentration (IC50) = 0.68 μg/mL) was higher than that of the reference ascorbic acid (IC50 = 0.79 μg/mL) and butylated hydroxytoluene (BHT) (IC50 = 0.88 μg/mL). The development of economical and efficient techniques is very important for the extraction and quantification of important pharmaceutical compounds such as KA.

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Section: Resultssupporting

confidence: 89%

Optimization of Ultrasonicated Kaempferol Extraction from Ocimum basilicum Using a Box–Behnken Design and Its Densitometric Validation

Altemimi

Mohammed

Lee

et al. 2020

Foods

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“…Rutin being a nontoxic and nonoxidizable molecule has advantages over other flavonoids such as myricetin, and quercetagenin that catalyzes the production of oxygen radical (Hodnick et al, 1986). Rutin has been quantitatively estimated by HPTLC method in different extracts of many plants such as Ficus species (Alajmi et al, 2015) and Ocimum sanctum Linn (Ilyas et al, 2015) as well as in pharmaceutical preparations (Soponar et al, 2010). HPLC has been also used for the estimation of rutin in the extracts of Althea rosea (Muhetaer et al, 2015), Syringa vulgaris (Toth et al, 2016) and tea (Porto-Figueira et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Comparative study of antioxidant activity and validated RP-HPTLC analysis of rutin in the leaves of different Acacia species grown in Saudi Arabia

Alam

Alajmi

Arbab

et al. 2017

Saudi Pharmaceutical Journal

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The present study assessed the comparative antioxidant potential of the ethanol extract (EE) of leaves of four species (, AS; , AL; AH; and , AT) grown in Saudi Arabia, including RP-HPTLC quantification of antioxidant biomarker rutin. DPPH radical scavenging and β-carotene-linoleic acid bleaching assays showed the promising antioxidant activities of extracts: ASEE (IC: 60.39 and 324.65 μg/ml) >ALEE (IC: 217.06 and 423.36 μg/ml) >ATEE (IC: 250.13 and 747.50 μg/ml) >AHEE (IC: 255.83 and 417.28 μg/ml). This was comparable to rutin tested at 500 μg/ml. Further, a RP- HPTLC densitometric method was developed (acetonitrile:water; 6:4; v/v) using glass-backed RP-18 silica gel F plate, and scanned at UV max 254 nm. The method was validated as per the ICH guidelines. Analysis of the validated RP-HPTLC displayed an intense peak ( = 0.65 ± 0.004) of rutin that was estimated (μg/mg dry weight) to be highest in ASEE (10.42), followed by ALEE (2.67), AHEE (1.36) and ATEE (0.31). Taken together, presence of rutin strongly supported the high antioxidant property of the tested species, especially. The developed RP-HPTLC method therefore, affirms its application in the quality control of commercialized herbal drugs or formulation containing rutin.

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Recent advances in methods of extraction, pre-concentration, purification, identification, and quantification of kaempferol

Norouzi,

Dastan,

Abdullah

et al. 2024

Journal of Chromatography A

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Densitometric Validation and Optimisation of Polyphenols in Ocimum sanctum Linn by High Performance Thin‐layer Chromatography

Cited by 7 publications

References 40 publications

Optimization of Ultrasonicated Kaempferol Extraction from Ocimum basilicum Using a Box–Behnken Design and Its Densitometric Validation

Optimization of Ultrasonicated Kaempferol Extraction from Ocimum basilicum Using a Box–Behnken Design and Its Densitometric Validation

Comparative study of antioxidant activity and validated RP-HPTLC analysis of rutin in the leaves of different Acacia species grown in Saudi Arabia

Recent advances in methods of extraction, pre-concentration, purification, identification, and quantification of kaempferol

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