Densitometric measurement of increased endothelial permeability in arteriosclerotic plaques and inhibition of permeability under the influence of two calcium antagonists

Effect of chronic nicotine delivery on the proliferation rate of endothelial and smooth muscle cells in experimentally induced vascular wall plaques

“…There is a direct correlation between the period of stimulation and the plaque size [13]. It has been shown by light and electron microscopic techniques that with this method no morphological changes of the EC are noted [10,13]. After 28 days of electrostimulation plaques with a maximum of 13.2_+ 3.8 intimal cell layers had developed.…”

“…With this method fibromuscular plaques develop in the intima of the arteries especially in the region of the anode. There is a direct correlation between the period of stimulation and the plaque size [13]. It has been shown by light and electron microscopic techniques that with this method no morphological changes of the EC are noted [10,13].…”

Influence of Several Solutions Used in Bypass Surgery on the Permeability of the Endothelium of Carotid Arteries in New Zealand Rabbits

“…This substance was given systemically to the animals by injection into an ear-vein (10000 units per kilo body weight). The circulation time of peroxidase was limited to 6 minutes [9]. Then the animals were sacrified by an overdose of narcotic (Hypnodil®) before starting the in-situ-fixation.…”

“…These pictures always included the intima with the endothelial monolayer, the subendothelial space, and the membrana elastica interna as regions of interest. The quantitative determination of the horseradish in these anatomic structures was performed by means of a procedure introduced by Strohschneider [9], where computerised densitometry evaluates the photonegatives of the prepared ultra-thin Strips. For standardization of each photonegative a correction factor was used so that the ratio of the relative densities of the membrana elastica interna and the subendothelial space was the same for all photonegatives.…”

Protective effect of flunarizine on blood-brain barrier permeability alterations in acutely hypertensive rats.

“…Flunarizine inhibited the increased endothelial permeability to horseradish peroxidase that occurred in arteriosclerotic plaques in response to electrical stimulation of the carotid arterial wall by implanted electrodes. 20 Another report 21 described suppression of atherogenesis in rabbit aortas following administration of oral flunarizine. In addition, flunarizine was reported to inhibit in a dose-related manner the enhanced vascular permeability in rat skin induced by histamine, serotonin, bradykinin, and arachidonic acid.…”