Dense tissue-like collagen matrices formed in cell-free conditions

Mosser, Gervaise; Anglo, Anny; Hélary, Christophe; Bouligand, Y.; Giraud‐Guille, Marie‐Madeleine

doi:10.1016/j.matbio.2005.09.002

Cited by 98 publications

(135 citation statements)

References 28 publications

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“…32 Decellularized ECM is a useful matrix for tissue engineering, because it can mimic the compositions, microstructure, and biomechanical properties of the native ECM. 14,33 Porcine tissues, including small intestine submucosa (SIS), bladder, dermis, pulmonary valves, adipose tissue, and tendons, have been used to overcome the supply and manufacturing limitations of human sources. [6][7][8][9][10][11]34 However, the ECM derived from porcine tissues may raise some concerns associated with immune reaction, as well as xenogeneic disease transmission.…”

Section: Discussionmentioning

confidence: 99%

Decellularized Extracellular Matrix Derived from Porcine Adipose Tissue as a Xenogeneic Biomaterial for Tissue Engineering

Choi

Kim

et al. 2012

Tissue Engineering Part C: Methods

110

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Cells in tissues are surrounded by the extracellular matrix (ECM), a gel-like material of proteins and polysaccharides that are synthesized and secreted by cells. Here we propose that the ECM can be isolated from porcine adipose tissue and holds great promise as a xenogeneic biomaterial for tissue engineering and regenerative medicine. Porcine adipose tissue is easily obtained in large quantities from commonly discarded food waste. Decellularization protocols have been developed for extracting an intact ECM while effectively eliminating xenogeneic epitopes and minimally disrupting the ECM composition. Porcine adipose tissue was defatted by homogenization and centrifugation. It was then decellularized via chemical (1.5 M sodium chloride and 0.5% sodium dodecyl sulfate) and enzymatic treatments (DNase and RNase) with temperature control. After decellularization, immunogenic components such as nucleic acids and a-Gal were significantly reduced. However, abundant ECM components, such as collagen (332.9 -12.1 mg/mg ECM dry weight), sulfated glycosaminoglycan (GAG, 85 -0.7 mg/mg ECM dry weight), and elastin (152.6 -4.5 mg/mg ECM dry weight), were well preserved in the decellularized material. The biochemical and mechanical features of a decellularized ECM supported the adhesion and growth of human cells in vitro. Moreover, the decellularized ECM exhibited biocompatibility, longterm stability, and bioinductivity in vivo. The overall results suggest that the decellularized ECM derived from porcine adipose tissue could be useful as an alternative biomaterial for xenograft tissue engineering.

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Section: Discussionmentioning

confidence: 99%

Decellularized Extracellular Matrix Derived from Porcine Adipose Tissue as a Xenogeneic Biomaterial for Tissue Engineering

Choi

Kim

et al. 2012

Tissue Engineering Part C: Methods

110

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“…We observe that varying the pH does not impact significantly the 1 H line widths for the native conditions, indicating that the structural changes induced by fibrillogenesis affect only moderately collagen dynamics reflected by the line width of the 1 H resonances (supplemental Table S1). Mosser et al (26) have shown that increasing the pH of a very dense collagen solution (400 -800 mg⅐ml Ϫ1 ) resulted in the formation of very small fibrils (ϳ5-15 nm of diameter). Nano-fibrils are also present within larger collagen I fibrils (5).…”

Section: Discussionmentioning

confidence: 99%

Solid-state NMR Study Reveals Collagen I Structural Modifications of Amino Acid Side Chains upon Fibrillogenesis

Peixoto¹,

Laurent²,

Azaı̈s³

et al. 2013

Journal of Biological Chemistry

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Background: Collagen fibrillogenesis is a fundamental process both in vivo and in vitro. Results: Fibrillogenesis increases the heterogeneity of conformations of side chain amino acids, impacting 40% of imino acids. Conclusion: A temperature-induced-attractive force component comes from significant amount of the collagen amino acids. Significance: This work brings new perspectives to studies of collagen assembly and interactions with other matrix molecules.

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“…To date, four distinct methods have been reported to modulate CFD: reverse dialysis [6], evaporation [7], plastic compression (PC) [8], and continuous injection [9]. These techniques allow for the fabrication of dense collagen (DC) gels with a fibrillar density within the range of 5e25 wt%, which have been used as 3D ECM models [10,11] and to engineer several tissues, such as osteoid [12e14], dura mater [15], nucleus pulpous [16,17], skin [18,19], bladder [20], cornea [21e24], tendon [25], airways [26], and blood vessels [26].…”

Section: Introductionmentioning

confidence: 99%

Fabrication of injectable, cellular, anisotropic collagen tissue equivalents with modular fibrillar densities

Marelli¹,

Ghezzi²,

James-Bhasin³

et al. 2015

Biomaterials

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Dense tissue-like collagen matrices formed in cell-free conditions

Cited by 98 publications

References 28 publications

Decellularized Extracellular Matrix Derived from Porcine Adipose Tissue as a Xenogeneic Biomaterial for Tissue Engineering

Decellularized Extracellular Matrix Derived from Porcine Adipose Tissue as a Xenogeneic Biomaterial for Tissue Engineering

Solid-state NMR Study Reveals Collagen I Structural Modifications of Amino Acid Side Chains upon Fibrillogenesis

Fabrication of injectable, cellular, anisotropic collagen tissue equivalents with modular fibrillar densities

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