Dense populations of the microsporidian Enterocytozoon hepatopenaei (EHP) in feces of Penaeus vannamei exhibiting white feces syndrome and pathways of their transmission to healthy shrimp

Tang, Kathy F.J.; Han, Jee Eun; Aranguren, Luis Fernando; White-Noble, Brenda; Schmidt, Margeaux M.; Piamsomboon, Patharapol; Risdiana, Eris; Hanggono, Bambang

doi:10.1016/j.jip.2016.08.004

Cited by 134 publications

(79 citation statements)

References 15 publications

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“…The changes observed by histopathological examinations in the hepatopancreatic tissue were in agreement with the previous reports (Tourtip et al, 2009;Tangprasittipap et al, 2013;Rajendran et al, 2016;Tang et al, 2016). The damage caused to the hepatopancreas affects the metabolic activities and growth of the shrimp, accounting for the retarded growth observed in shrimp ponds with high EHP incidence.…”

Section: Ehp-510r Gcgtactatccccagagcccgasupporting

confidence: 91%

“…The usage of live feeds poses significant threat to shrimp culture; the infected live feeds can infect and spread EHP through feces (Newman, 2015). Tangprasittpap et al, (2013) and Tang et al, (2016) performed the feeding and cohabitation bioassay, and stated that EHP can be transmitted horizontally through cannibalism. Salachanet al, (2017) stated that EHP can be directly transmitted to other shrimps via water.…”

Section: Risk Factors In Disease Preventionmentioning

confidence: 99%

“…However, their activity to prevent/reduce EHP infection is still uncertain. Tang et al, (2016) found that usage of Fumagillin-B, an antimicrobial agent is ineffective in reducing or eliminating EHP spores in infected shrimps and reported that, farmers of Indonesia followed two types of management strategies to prevent EHP and WFS. 1.…”

Section: Role Of Management In Disease Preventionmentioning

confidence: 99%

“…EHP was first reported from Penaeus monodon in Thailand (2009) and subsequently from China, Indonesia, Thailand, Malaysia, Vietnam and India (Joshi et al, 2014;Rajendran etal., 2016;Thitamadee et al, 2016). Although EHP has not been associated with mortality, recent studies clearly indicate that EHP is associated with growth retardation in P. vannamei and P. monodon (Tang et al, 2016;Giridharan and Uma, 2017).…”

Section: Introductionmentioning

confidence: 99%

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Incidence of Hepatopancreatic Microsporidiasis, by Enterocytozoon hepatopenaei (EHP) in Penaeus vannamei Culture in Nellore District, Andhra Pradesh, India and the Role of Management in its Prevention and Transmission

Kummari¹,

Haridas²,

Handique³

et al. 2018

Int.J.Curr.Microbiol.App.Sci

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Shrimp culture is the most lucrative aquaculture sector in Asia-Pacific region. Recently, shrimp farms in India have been affected by the microsporidian parasite, Enterocytozoon hepatopenaei (EHP) associated with retarded growth without other clinical signs, causing significant economic losses to the shrimp industry. The present study was carried out to investigate the incidence of EHP in Penaeus vannamei farms of Nellore district of Andhra Pradesh, and to identify the carriers of EHP and the role of management in reducing the incidence of EHP in ponds. PCR analysis by two sets of EHP specific primers indicated the incidence of EHP to be 92.50%. Histopathological studies of the hepatopancreas of affected shrimp showed sloughing of the epithelial cells of hepatopancreatic tubules, lifting and detachment of epithelial layers of hepatopancreatic tubules and heavy damage of hepatopancreas. PCR screening of aquatic macro fauna in the farms showed that samples of crabs, polychaetesand non-penaeids were positive for EHP, implying that they could act as carriers of EHP. On the other hand, fishes were found negative. Strict implementation of Best Management Practices (BMPs) in one selected farm which was confirmed to be infected by EHP clearly showed that practice of BMPs and avoidance of carriers can definitely reduce the incidence of EHP during the subsequent culture in shrimp farms. After implementation of BMPs, significant improvement in growth rate, and Feed Conversion Ratio (FCR) were also observed. In the control pond where BMPs were not implemented, EHP continued to be present, affecting growth and FCR adversely. This is the first report comparing the role of management in EHP infection and identifying carriers of EHP in P. vannamei culture in India. K e y w o r d sEnterocytozoon hepatopenaei,

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Section: Ehp-510r Gcgtactatccccagagcccgasupporting

confidence: 91%

Section: Risk Factors In Disease Preventionmentioning

confidence: 99%

Section: Role Of Management In Disease Preventionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Incidence of Hepatopancreatic Microsporidiasis, by Enterocytozoon hepatopenaei (EHP) in Penaeus vannamei Culture in Nellore District, Andhra Pradesh, India and the Role of Management in its Prevention and Transmission

Kummari¹,

Haridas²,

Handique³

et al. 2018

Int.J.Curr.Microbiol.App.Sci

View full text Add to dashboard Cite

show abstract

“…In Indonesia, local producers applying Probiotics, Garlic paste (10-20g/kg feed) and vitamin C (2g/kg feed) to avoid EHP and WFS in shrimp culture (Tang et al, 2016) …”

Section: Treatmentmentioning

confidence: 99%